口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (6): 642-645.DOI: 10.13701/j.cnki.kqyxyj.2017.06.016

• 临床研究论著 • 上一篇    下一篇

CPP-ACP/ACFP预处理的牙本质与树脂粘结剂粘结作用的研究

魏晓慧, 刘利君#, 刘红*, 宋效庆, 陈天杰, 刘称称   

  1. 吉林大学口腔医院吉林省牙发育及颌骨重塑与再生重点实验室 吉林 长春 130021
  • 收稿日期:2017-01-01 出版日期:2017-06-20 发布日期:2017-06-26
  • 通讯作者: 刘红,电话:0431—85579506#为共同第一作者
  • 作者简介:魏晓慧(1988~ ),女,河北省衡水市人,硕士,医师,现工作于中国人民解放军第307医院。刘利君(1987~ ),女,黑龙江省齐齐哈尔市人,硕士,医师,主要从事口腔材料学研究工作。
  • 基金资助:
    吉林省教育厅“十二五”科学技术研究规划项目资助(吉教科合字[2015]527号)

Effect CPP-ACP/ACFP Pretreatment on Bond Strength of Resin Cements and Dentin.

WEI Xiao-hui, LIU Li-jun#, LIU Hong*, SONG Xiao-qing, CHEN Tian-jie, LIU Chen-chen.   

  1. The Key Laboratory of Tooth Development and Bone Remodeling &
    Regeneration, School and Hospital of Stomatology, Jilin University, Changchun 130021, China
  • Received:2017-01-01 Online:2017-06-20 Published:2017-06-26

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被引次数

2

摘要： 目的:通过设立对比实验,研究酪蛋白磷酸肽-无定形磷酸钙(Casein phosphopetide-amorphic calcium phosphat,CPP-ACP)及酪蛋白磷酸肽-无定形磷酸氟钙(Casein phosphoetide-amorphic calcium fluoride phosphate complexes,CPP-ACFP)对牙本质小管的封闭效果及对树脂粘结剂粘结强度的影响。方法:将36个试件随机分为自酸蚀和全酸蚀组,每组再分空白组、GC组、GC plus组3个亚组(n=6)。采用扫描电子显微镜(SEM)观察CPP-ACP和CPP-ACFP预处理牙本质1 d和7 d后的表面形貌改变;用能谱分析,计算钙磷比值,分析羟基磷灰石晶体结构和成份;每个试件上均堆积厚约4 mm的Z350树脂,通过万能测试机进行微拉伸强度测试,分析CPP-ACP和CPP-ACFP预处理对粘结剂粘结强度的影响;使用体视显微镜,观察界面的断裂模式,并对结果进行统计分析。结果:SEM显示,经预处理的牙本质小管直径减小,经多次预处理与1次相比,牙本质小管几乎完全封闭;能谱分析表明经预处理的牙本质与空白组牙本质Ca∶P比值变化并无统计学差异,新形成的羟基磷灰石结构和成份与牙本质类似;微拉伸实验结果显示,在牙本质表面应用CPP-ACP或CPP-ACFP后,可降低全酸蚀粘结剂single bond2的粘结强度(P＜0.05),而对自酸蚀粘结剂ibond的粘结强度无明显影响;两者对同一种粘结剂粘结强度的影响无统计学意义;体视显微镜观察,各组断裂模式均以粘结面断裂为主。结论:粘结前使用CPP-ACP或CPP-ACFP预处理牙本质表面,可以封闭牙本质小管,多次应用效果更加显著;CPP-ACP或CPP-ACFP预处理后对自酸蚀粘结剂粘结强度没有影响;会降低全酸蚀粘结剂的粘结强度。

关键词: 牙本质敏感, 粘结强度, 微拉伸强度, 酪蛋白磷酸肽

Abstract: Objective: To evaluate the effect of pretreatment by Casein phosphopetide-amorphic calcium phosphate (CPP-ACP)and Casein phosphoetide-amorphic calcium fluoride phosphate complexes (CPP-ACFP) on the bond strength of resin cements and resin. Methods: Thirty-six specimens were randomly divided into self-etching and total-etching group, and each group was constituted with blank group, GC group and GC plus group (n = 6). After pretreated by CPP-ACP and CPP-ACFP on day 1 and day 7, the morphology of dentin surface was observed by scanning electron microscope (SEM). Calcium/phosphorus ratio was evaluated by energy dispersive spectroscopy analysis. A 4 mm-thick layer of Z350 resin was placed on each specimen, and the micro tensile strength was tested by the universal testing machine. The fracture mode of interface was observed. Results: results showed that the diameter of pretreatment dentin tubule decreased, and the dentin tubule almost completely closed after several times of repeated pretreatment. The Ca/P ratio changes were not statistically different between the pretreated dentin and normal dentine. The bond strengths of total-etching adhesive system (single bond2) were decreased (P<0.05) by pretreatment of CPP-ACP or CPP-ACFP. The pretreatment had no obvious effect on self-etching adhesive system (ibond). There were no differences between CPP-ACP and CPP-ACFP pretreatment in both etching adhesive systems. Stereoscopic microscope showed that the fracture mode of interface was mainly on the bonding surface. Conclusion: Pretreatment of dentine by CPP-ACP or CPP-ACFP before bonding could close the dentin tubule. Increase times of application might improve this effect. Pretreatment of dentine by CPP-ACP or CPP-ACFP had no obvious effect on self-etching adhesive system (ibond), while could reduce bond strength of total-etching adhesive system.

Key words: Dentine hypersensitivity , Bond strength , Tensile strength , Casein phosphoetide

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