两种抗氧化剂凝胶对内漂白后充填树脂微渗漏的影响

doi:10.13701/j.cnki.kqyxyj.2023.08.013

摘要/Abstract

摘要： 目的: 对比含表面活化剂(0.2%Tween 80)的10%抗坏血酸钠(sodium ascorbate,SA)凝胶和6.5%原花青素(proanthocyanidins,PC)凝胶对死髓牙内漂后充填树脂微渗漏的影响,以及确定最佳处理时间。方法: 收集近1月拔除的离体前磨牙120颗,随机分为10组(n=12), A组:未漂白组,B组:漂白组, C组:含0.2%Tween 80的10%SA组,D组:6.5%PC组,C/D组根据内漂白后抗氧化剂处理时间不同(10、30、60、120 min)又分为4个亚组(C1/C2/C3/C4,D1/D2/D3/D4),各组处理后行树脂充填窝洞。所有样本经过冷热循环后,每组随机选取10个样本置于2%亚甲基蓝染色,在体式显微镜下观察树脂与洞壁粘接界面微渗漏的程度,并对测量结果进行统计学分析。每组剩余两个样本于扫描电镜下观察牙体组织与充填树脂粘接界面的微观形貌特点。结果: A组微渗漏值明显小于B组(P=0.000),各实验组与B组比较差异均有统计学意义(P<0.05),C组微渗漏值逐渐减小,D3组微渗漏值最小, C/D组间除C4/D4外其余均有统计学差异(P<0.05);SEM观察可见,B组粘接界面可见明显间隙,且裂隙范围最大, C组粘接界面间隙逐渐变窄,C4组间隙消失, D组粘接界面更加紧密,且形成了良好的混合层。结论: 6.5%PC凝胶对死髓牙内漂后充填树脂微渗漏的处理效果更佳,建议最佳处理时间为60 min时。

关键词: 抗坏血酸钠凝胶, 原花青素凝胶, 牙齿内漂白, 微渗漏

Abstract: Objective: To compare the influence of 10% SA gel with a surfactant (0.2%Tween 80) and 6.5% PC gel on the microleakage of filling resin after intracoronal bleaching and determine the best treating time. Methods: One hundred and twenty premolars extracted in the past one month were collected and randomly divided into ten groups (n=12). Group A: unbleached group, Group B: bleached group, Group C: 10% SA gel with 0.2% Tween 80, Group D: 6.5% PC gel. After intracoronal bleaching,each experimental group was divided into 4 subgroups(C1/C2/C3/C4 and D1/D2/D3/D4) according to the treating time of antioxidant (10 min, 30 min, 60 min, and 120 min). Then the cavity was filled with composite resin. After 500 thermal cycles, 10 randomly selected samples in each group were put into 2% methylene blue for 24 hours. The microleakage of filled resin was observed at buccolingual under stereomicroscope. The measured data was analyzed with statistical methods. The micromorphology of the bonding interface in the remaining samples was observed with scanning electron microscope(SEM). Results: Group A showed a significantly lower microleakage compared to group B (P=0.000). There were statistical differences between the experimental groups and group B,the microleakage of the filled resin was gradually decreased, and the lowest value was group D3. There was apparent significance between C1 and D1, C2 and D2, C3 and D3 (P<0.05), except for C4 and D4 (P=0.089). Group B demonstrated the most obvious and largest gap between filling resin and dental tissues observed by SEM. The gap at the bonding interface was gradually narrowed in group C, and disappeared in group C4. The filling resin was well united with dental tissue,and the adhesion belt was well formed in group D. Conclusion: 6.5% PC gel has a better effect on the microleakage of filling resin after internal bleaching of dead pulp teeth. It is suggested that the best treatment time is 60 minutes.

Key words: sodium ascorbate gel, proanthocyanidins gel, intracoronal bleaching, microleakage

张小平, 常悦. 两种抗氧化剂凝胶对内漂白后充填树脂微渗漏的影响[J]. 口腔医学研究, 2023, 39(8): 732-737.

ZHANG Xiaoping, CHANG Yue. Influence of Two Antioxidants on Microleakage of Filling Resin after Intracoronal Bleaching[J]. Journal of Oral Science Research, 2023, 39(8): 732-737.

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