人脐带间充质干细胞在壳聚糖温敏凝胶中增殖和成骨分化的实验研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (1): 38-40.

人脐带间充质干细胞在壳聚糖温敏凝胶中增殖和成骨分化的实验研究

王一波,李云鹏,侯锐^*,刘彦普^*

第四军医大学口腔医院口腔颌面外科,军事口腔医学国家重点实验室陕西西安 710032

收稿日期:2014-05-12 出版日期:2015-01-28 发布日期:2016-04-29
通讯作者: 侯锐,电话:029-84776102
作者简介:王一波(1984~ ),男,山东人,在读博士,研究方向为基于组织工程的组织缺损修复。
基金资助:
国家自然科学基金资助项目(编号:81100792)

Proliferation and Osteogenic Differentiation of Human Umbilical Cord Mesenchymal Stem Cell in Chitosan Thermosensitive Hydrogel.

WANG Yi-bo, LI Yun-peng, HOU Rui, et al

State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University, Xian 710032

Received:2014-05-12 Online:2015-01-28 Published:2016-04-29

摘要/Abstract

摘要： 目的:研究人脐带间充质干细胞(human umbilical cord mesenchymal stem cells, hUCMSCs)在壳聚糖温敏凝胶中的增殖和成骨分化。方法:体外分离培养hUCMSCs。制备壳聚糖温敏凝胶并使用扫描电镜观察其超微结构。用壳聚糖温敏凝胶浸提液培养细胞,MTT法检测细胞增殖情况。使用成骨诱导培养液诱导壳聚糖温敏凝胶中生长的hUCMSCs向成骨方向分化,在诱导过程中进行茜素红法染色钙结节(21 d)。结果:hUCMSCs在壳聚糖温敏凝胶浸提液中的增殖情况与常规培养液中相近,无显著性差异。壳聚糖温敏凝胶中生长的hUCMSCs成骨诱导21d时可见到橘红色的钙结节。结论:hUCMSCs在壳聚糖温敏凝胶中可以正常增殖,经过诱导后可以向成骨方向分化。[关键词] 脐带间充质干细胞壳聚糖温敏凝胶增殖成骨分化

关键词: 脐带间充质干细胞, 壳聚糖温敏凝胶, 增殖, 成骨分化

Abstract: Objective: To investigate the proliferation and osteogenic differentiation of human umbilical cord mesenchymal stem cells in chitosan thermosensitive hydrogel. Methods: Human umbilical cord mesenchymal stem cells were isolated and cultured in vitro. The chitosan thermosensitive hydrogel was visualized by scanning electron microscopy. Then the cells were cultured in chitosan thermosensitive hydrogel extracts and given osteogenic induction. The proliferation was measured by MTT assay. Alizarin red staining at 21d was performed to assess the osteogenic differentiation potential of hUCMSCs. Results: In chitosan thermosensitive gel, the proliferation of hUCMSCs was similar with those in common medium, and there was no significant difference. Alizarin red staining was positive with formation of calcium nodules after osteogenic induction (21d). Conclusion: HUCMSCs could proliferate and have osteogenic differentiation potential in chitosan thermosensitive hydrogel.

Key words: Human umbilical cord mesenchymal stem cell , Chitosan thermosensitive hydrogel , Proliferation, Osteogenic differentiation

中图分类号:

R782.2

王一波,李云鹏,侯锐,刘彦普. 人脐带间充质干细胞在壳聚糖温敏凝胶中增殖和成骨分化的实验研究[J]. 口腔医学研究, 2015, 31(1): 38-40.

WANG Yi-bo, LI Yun-peng, HOU Rui, et al. Proliferation and Osteogenic Differentiation of Human Umbilical Cord Mesenchymal Stem Cell in Chitosan Thermosensitive Hydrogel.[J]. Journal of Oral Science Research, 2015, 31(1): 38-40.

参考文献

[1] Peng J, Wang Y, Zhang L, et al. Human umbilical cord Wharton's jelly-derived mesenchymal stem cells differentiate into a Schwann-cell phenotype and promote neurite outgrowth in vitro [J]. Brain Res Bull, 2011, 84(3)∶235-243
[2] Troyer DL, Weiss ML. Wharton's jelly-derived cells are a primitive stromal cell population [J]. Stem Cells, 2008, 26(3)∶591-599
[3] Nekanti U, Rao VB, Bahirvani AG, et al. Long-term expansion and pluripotent marker array analysis of Wharton's jelly-derived mesenchymal stem cells [J]. Stem Cells Dev, 2010, 19(1)∶117-130
[4] Ahmadi R, de Bruijn JD. Biocompatibility and gelation of chitosan-glycerol phosphate hydrogels [J]. J Biomed Mater Res A, 2008, 86(3)∶824-832
[5] Song K, Qiao M, Liu T, et al. Preparation, fabrication and biocompatibility of novel injectabletemperature-sensitive chitosan/glycerophosphate/collagen hydrogels [J]. J Mater Sci Mater Med, 2010, 21(10)∶2835-2842
[6] Kadam SS, Tiwari S, Bhonde RR. Simultaneous isolation of vascular endothelial cells and mesenchymal stem cells from the human umbilical cord [J]. In Vitro Cell Dev Biol Anim, 2009, 45(1-2)∶23-27
[7] Cho PS, Messina DJ, Hirsh EL, et al. Immunogenicity of umbilical cord tissue derived cells [J]. Blood, 2008, 111(1)∶430-38
[8] Yan J, Yang L, Wang G, et al. Biocompatibility evaluation of chitosan-based injectable hydrogels for the culturing mice mesenchymal stem cells in vitro [J]. J Biomater Appl, 2010, 24(7)∶625-37
[9] Richardson SM, Hughes N, Hunt JA, et al. Human mesenchymal stem cell differentiation to NP-like cells in chitosan-glycerophosphate hydrogels [J]. Biomaterials, 2008, 29(1)∶85-93
[10] Deng C, Zhang P, Vulesevic B, et al. A collagen-chitosan hydrogel for endothelial differentiation and angiogenesis [J]. Tissue Eng Part A, 2010, 16(10)∶3099-3109
[11] Lü S, Wang H, Lu W, et al. Both the transplantation of somatic cell nuclear transfer- andfertilization-derived mouse embryonic stem cells with temperature-responsivechitosan hydrogel improve myocardial performance in infarcted rat hearts [J]. Tissue Eng Part A, 2010, 16(4)∶1303-1315
[12] 刘昌奎,侯锐,胡开进,等.应用加载双向诱导分化的hUCMSCs的壳聚糖温敏凝胶促进移植牙根-骨牙周膜愈合的研究展望[J].口腔医学研究,2013,29(7)∶688-690

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