口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (2): 105-108.

• 基础研究论著 • 上一篇    下一篇

siRNA干扰对变形链球菌耐氟菌ffh基因产酸能力的影响

李朋莲1,张志民*,李振玲2,张桐菲3,李天博4,闫胜男5,戴莹6   

  1. 吉林大学口腔医院牙体牙髓科 吉林 长春 130021
  • 收稿日期:2014-09-24 出版日期:2015-02-28 发布日期:2016-04-29
  • 通讯作者: 张志民,Email:zhangzm1964@sina.com
  • 作者简介:李朋莲(1989~ ),女,朝鲜族,吉林省白山市人,硕士在读,主要从事龋病病因学及临床研究。
  • 基金资助:
    国家自然科学基金(3A411X463431)

Effect of siRNA Interference on the Acidogenicity for Gene ffh of Fluoride Resistant Streptococcus Mutans.

LI Peng-lian, ZHANG zhi-min, LI Zhen-ling, et al   

  1. Hospital of Stomatology, Jilin University, Changchun, 130021
  • Received:2014-09-24 Online:2015-02-28 Published:2016-04-29

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摘要： 目的:探讨变形链球菌耐氟菌(UA159-FR)ffh基因 siRNA干扰后对产酸能力的影响。方法:电穿孔法将siRNA转入UA159-FR与ffh基因序列靶向位点结合,将干扰前后细菌分别加入BHI培养液,含5%糖类的葡萄糖、蔗糖、乳糖、淀粉中培养,pH调至7.5,24 h后测终末pH。结果:转染结果成功;干扰前后不同糖培养基中变形链球菌耐氟菌ffh基因对产酸有显著差异(P<0.05);干扰前后在常规、葡萄糖、及乳糖中差异极显著(P<0.01);蔗糖中差异显著(P<0.05);淀粉中无显著差异。结论:变形链球菌耐氟菌中基因ffh对产酸能力影响显著。

关键词: 变形链球菌耐氟, ffh基因, siRNA, 产酸能力

Abstract: Objective: To have a discussion on the effect on the acidogenicity for gene ffh of fluoride resistant streptococcus mutans (UA159-FR)after siRNA interference. Methods: UA159-FR was transformed by using electroporation and combined with targeted site of ffh gene sequence, and cultured by adding BHI, glucose, saccharose, lactose and starch with 5% saccharides into bacteria that was before interfered and after interfered respectively. PH was set to 7.5. The terminal PH was measured after 24 hours. Results: After the transfection, the result turned out to be successful. Before and after interference, there were some significant differences for transfection of fluoride resistant streptococcus mutans among different culture media of gene ffh (P<0.05).△PH of normal, glucose and lactose were significantly different before and after the interference(P<0.01); It was significant in sucrose(P<0.05) and there was no obvious difference in starch. Conclusion: The effect of siRNA interference on the acidogenicity for gene ffh of fluoride resistant streptococcus mutans is significant.

Key words: Fluoride resistant streptococcus mutans, ffh gene siRNA, Acidogenicity

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