口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (3): 226-229.

• 基础研究论著 • 上一篇    下一篇

新型GBR胶原膜的早期动物体内植入实验研究

白冰1, 朱静涛2*, 冯丽芳1, 宋荣博3   

  1. 1.中国医科大学口腔医学院修复科,辽宁省口腔医学研究所 辽宁 沈阳 110001;
    2. 中国医科大学口腔医学院奉天门诊;
    3. 中国医科大学口腔医学院中心实验室
  • 收稿日期:2014-07-24 出版日期:2015-03-28 发布日期:2016-04-29
  • 通讯作者: 朱静涛,E-mail:zhujt78@163.com
  • 作者简介:白冰(1978~ ),女,辽宁丹东人,博士,副主任医师,主要从事骨组织工程研究。
  • 基金资助:
    辽宁省自然科学基金(2013021038)

Guided Bone Regeneration in Mandibular Defects Using a Novel Collagen Membrane.

BAI Bing, ZHU Jing-tao, FENG Li-fang,et al.   

  1. Department of Prosthodontics, School of Stomatology, China Medical University, Liaoning Institute of Dental Research, Shenyang 110001
  • Received:2014-07-24 Online:2015-03-28 Published:2016-04-29

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摘要： 目的:探讨新型国产GBR胶原膜体内植入后,诱导早期膜下成骨的能力。方法:实验于2013年10月~2014年3月在沈阳军区总医院动物实验中心完成。选取小型巴马猪,于双侧下颌骨骨体处用牙科裂钻制备8mm×8mm全层骨缺损3个,分别应用实验胶原膜、Bio-gide@、无覆盖膜覆盖骨缺损。术后1个月处死动物,分别在处死前1、2周分别肌肉注射四环素溶液与二甲酚橙溶液。固定样本后,制备硬组织切片。分别在荧光显微镜及光学显微镜下(甲苯胺蓝、亚甲基蓝-酸性品红染色)观测膜的降解程度及膜下新骨生成能力,评价材料膜下骨形成量和骨成熟程度。结果:实验组胶原膜具备良好的屏障作用,膜下新生骨矿化程度良好;骨小梁排列整齐,但新生骨量少于对照组。 结论:新型国产胶原膜在1个月时无明显降解,具备良好的膜下成骨能力,需进行实验组胶原膜的改性,以增加膜下成骨量。

关键词: 胶原膜, 引导骨再生, 新骨生成

Abstract: Objective: To evaluate a new synthetic degradable barrier membrane based on collagen for use in guided bone regeneration. Methods: The collagen membrane was embedded in Bama pigs mandibular bone and Bio-gide@ membrane served as reference materials. In Bama pigs, three standardized 8mm×8mm defect was created in mandibular bone. Three groups (collagen, Bio-gide@, no membrane) were evaluated at 4 weeks. New bone formation was demonstrated by histological analysis. Results: In the Bio-gide@ and collagen groups, the defects were incompleted new formedbone filling. In contrast to uncovered mandibular defects, substantial bone healing was observed in defects covered with a barrier membrane. In the non membrane covered case, the formation of bone was unformed in 4 weeks. There were new formed bone under the Bio-gide@ and collagen membranes. The amount of new bone formed under the Bio-gide@ membranes were more than the amount of bone formed under the collagen membranes. Conclusion: It can be concluded that the collagen membrane was well suited for use in guided bone regeneration.

Key words: Collagen membrane, Guided bone regeneration (GBR), New bone formation

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