维生素C促进根尖牙乳头间充质干细胞组蛋白去甲基化酶KDM2B和KDM5C表达的研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (5): 466-469.

维生素C促进根尖牙乳头间充质干细胞组蛋白去甲基化酶KDM2B和KDM5C表达的研究

王月君¹, 刘大勇¹, 范志朋², 贾智^1*

王月君¹ 刘大勇¹ 范志朋² 贾智^1*(1. 天津医科大学口腔医院牙体牙髓科天津 300070;
2. 首都医科大学附属北京口腔医院

收稿日期:2014-10-17 出版日期:2015-05-28 发布日期:2016-04-29
通讯作者: 贾智,Email: jiazhi1962@tijmu.edu.cn
作者简介:王月君(1987~ ),女,硕士,主要从事口腔内科学临床及细胞分子生物学研究。
基金资助:
国家自然基金面上项目(编号:81371109)

Vitamin C Enhanced the Expressions of Histone Demethylase KDM2B and KDM5C in Stem Cells from Apical Papilla.

WANG Yue-jun, LIU Da-yong, FAN Zhi-peng, et al.

Department of Endodontics, Tianjin Medical University School of Stomatology, Tianjin 300070

Received:2014-10-17 Online:2015-05-28 Published:2016-04-29

摘要/Abstract

摘要： 目的:研究维生素C是否具有调节根尖牙乳头间充质干细胞组蛋白去甲基化酶相关基因表达的功能。方法:利用不同剂量的维生素C刺激人根尖牙乳头间充质干细胞;采用qRT-PCR(real time reverse transcription-polymerase chain reaction,实时定量RT-PCR)在mRNA水平检测组蛋白去甲基化酶相关基因的表达。结果:qRT-PCR结果显示组蛋白去甲基化酶KDM2B(Lysine (K)-specific demethylase 2B)和KDM5C (Lysine (K)-specific demethylase 5C)在10 μmol/L维生素C刺激后2、4和8 h表达明显升高,其表达升高程度与维生素C的作用具有时依赖性;qRT-PCR结果显示与未刺激组相比,5、10和20 μmol/L维生素C在作用8 h后都能促进 KDM2B和KDM5C的表达,其表达升高程度与维生素C的作用具有剂量依赖性的关系。结论:在根尖牙乳头间充质干细胞中维生素C可以促进组蛋白去甲基化酶KDM2B和KDM5C的表达。

关键词: 维生素C, 组蛋白去甲基化酶, 根尖牙乳头间充质干细胞, KDM2B, KDM5C

Abstract: Objective: To investigate whether Vitamin C could regulate the expression of histone demethylases in stem cells from apical papilla (SCAPs). Methods: Different doses of Vitamin C were used to stimulate the SCAPs. The qRT-PCR was used to detect the expression of histone demethylases at the mRNA level. Results: The qRT-PCR results showed that the expressions of KDM2B and KDM5C were up-regulated at 2, 4 and 8h after 10 μmol/L Vitamin C treatment in a time dependent manner. And 5, 10 and 20 μmol/L Vitamin C could increase the expressions of KDM2B and KDM5C in a dose dependent manner at 8h. Conclusion: Vitamin C could induce the expressions of histone demethylase KDM2B and KDM5C in SCAPs.

Key words: Vitamin C , Histone demethylase, SCAPs, KDM2B , KDM5C

中图分类号:

R780.1

王月君, 刘大勇, 范志朋, 贾智. 维生素C促进根尖牙乳头间充质干细胞组蛋白去甲基化酶KDM2B和KDM5C表达的研究[J]. 口腔医学研究, 2015, 31(5): 466-469.

WANG Yue-jun, LIU Da-yong, FAN Zhi-peng, et al.. Vitamin C Enhanced the Expressions of Histone Demethylase KDM2B and KDM5C in Stem Cells from Apical Papilla.[J]. Journal of Oral Science Research, 2015, 31(5): 466-469.

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