银杏叶提取物对骨髓间充质干细胞增殖及成骨分化的影响

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (6): 575-579.

银杏叶提取物对骨髓间充质干细胞增殖及成骨分化的影响

张甲第¹, 时舒曼¹, 张海洋¹, 谷旭², 高幸¹, 吴哲^1*

1. 吉林大学口腔医学院修复科吉林长春 130021;
2. 解放军208医院461临床部

收稿日期:2014-10-30 出版日期:2015-06-28 发布日期:2016-04-29
通讯作者: 吴哲,E-mail:wuz@jlu.edu.cn
作者简介:张甲第(1990~ ),女,黑龙江七台河人,硕士,主要从事口腔修复学研究。
基金资助:
吉林省自然科学基金资助课题(201215052);吉林省产业技术研究与开发项目(2013C023-5)

Effects of GBE on the Proliferation and Osteogenic Differentiation of BMSCs.

ZHANG Jia-di, SHI Shu-man, ZHANG Hai-yang, et al.

Department of Prosthodontics, School of Stomatology, JiLin University, Changchun 130021

Received:2014-10-30 Online:2015-06-28 Published:2016-04-29

摘要/Abstract

摘要： 目的:研究银杏叶提取物(ginkgo biloba extract ,GBE)对大鼠的骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)增殖及成骨分化过程的影响。方法:将不同浓度的GBE作用于第3代BMSCs,通过CCK-8(Cell Counting Kit-8)法及流式细胞仪法检测其对细胞增殖活性变化的影响,通过茜素红染色及碱性磷酸酶(Alkalinet phosphatase,ALP)法判定其对细胞成骨能力的影响。RT-PCR检测成骨相关基因骨形成蛋白2(bone morphogenetic protein-2, BMP-2)、Runx2(runt-related transcription factor2)的mRNA的表达。结果:不同浓度的GBE对BMSCs的增殖无明显影响,但能使细胞处于S期,显著促进ALP的表达,在浓度为150 mg/L时表达最多。其成骨相关基因BMP-2、Runx2的mRNA表达也相对增高。结论:银杏叶提取物对骨髓间充质干细胞的成骨分化具有积极意义。

关键词: 银杏叶提取物, 骨髓间充质干细胞, 成骨分化, 增殖

Abstract: Objective: To study the effects of GBE on proliferation and osteogenic differentiation process of rat BMSCs. Methods: By applying different concentrations of GBE on the third-generation of BMSCs,we evaluate its influence on the cell proliferation activity through CCK-8 assay and flow-cytometry analysis. Then we estimated the effect of osteogenic ability of GBE by alizarin red S and alkalinet phosphatase method. RT-PCR was taken to test the expression of the osteogenic specific genes (BMP-2, Runx2) mRNA. Results: There were no statistically significant differences between different concentrations of ginkgo biloba extract in the proliferation on bone marrow mesenchymal stem cells. However, GBE could make the cells in S phase of the cell cycles. GBE could significantly promote the expression of alkaline phosphatase, especially when the concentration was 150 mg/L. The osteogenic specific genes(BMP-2,Runx2)mRNA expression were also relatively higher than control group. Conclusion: Ginkgo biloba extract has a significantly positive effect on the osteogenic differentiation of the BMSCs.

Key words: Ginkgo biloba extract, Bone marrow mesenchymal stem cell , Osteogenic differentiationProliferation

中图分类号:

R782

张甲第, 时舒曼, 张海洋, 谷旭, 高幸, 吴哲. 银杏叶提取物对骨髓间充质干细胞增殖及成骨分化的影响[J]. 口腔医学研究, 2015, 31(6): 575-579.

ZHANG Jia-di, SHI Shu-man, ZHANG Hai-yang, et al.. Effects of GBE on the Proliferation and Osteogenic Differentiation of BMSCs.[J]. Journal of Oral Science Research, 2015, 31(6): 575-579.

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