不同加载频率拉应力对髁突软骨细胞增殖分化的影响

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (7): 662-665.

不同加载频率拉应力对髁突软骨细胞增殖分化的影响

王亚斐,王思涵,郭小凯,刘畅^*

吉林大学口腔医院正畸科吉林长春 130021

收稿日期:2014-12-05 出版日期:2015-07-28 发布日期:2015-07-28
通讯作者: 刘畅,电话:0431-88796023
作者简介:王亚斐(1987~ ),女,山东人,医师,硕士,主要从事口腔正畸临床及基础研究。
基金资助:
吉林省卫生厅资助项目(编号:2008Z010);吉林大学基本科研业务费-科学前沿与交叉学创新项目(编号:200903225)

Effect of Different Frequency Tensile Stress on the Proliferation and Differentiation of Mandibular Condylar Chondrocytes

WANG Ya-fei, WANG Si-han, GUO Xiao-kai, et al

Department of Orthodontics, Stomatological Hospital, Jilin Univeristy. Changchun 130021

Received:2014-12-05 Online:2015-07-28 Published:2015-07-28

摘要/Abstract

摘要： 目的:探讨不同加载频率的拉应力对髁突软骨细胞增殖、分化的影响。方法:体外分离2周龄新西兰兔髁突软骨细胞,对细胞施加2 h/d,为期3 d的不同频率(0 Hz、0.1 Hz、0.5 Hz、1 Hz)的拉应力。采用CCK8检测细胞增殖水平,实时荧光定量RT-PCR检测Sox9、Runx2、VEGF的表达。结果:细胞增殖水平在静止性(0 Hz)组显著高于周期性加力(0.1~1 Hz)组和对照组;Sox9、Runx2、VEGF表达在0.5 Hz组和1 Hz组显著高于静止性(0 Hz)组和对照组。结论:不同加载频率拉应力对髁突软骨细胞增殖分化有差异性影响。

关键词: 软骨内成骨, 周期性拉应力, 静止性拉应力, 增殖, 分化

Abstract: Objective: To investigate the effect of different frequency of tensile stress on the proliferation and differentiation of mandibular condylar chondrocytes. Methods: Mandibular condylar chondrocytes (MCC) from two-week-old New Zealand rabbits were cultured in vitro under different frequency(0.1Hz,0.5Hz,1Hz) tensile stress for a period of 2 h per day for 3 days .The proliferation of articular chondrocyte was determined by CCK-8 assay. The gene expression (Sox9, Runx2, VEGF )was analyzed using real-time RT-PCR. Results: The static(0HZ) tensile stress had a significant stimulate on the chondrocyte proliferation compared with periodic tensile stress(0.1-1HZ)and the control group. The expression of Sox9, Runx2 and VEGF under 0.5HZ and 1HZ was significantly higher than the static(0HZ) tensile stress and the control group. Conclusion: There are different effects on the proliferation and differentiation of MCC under different frequency tensile stress.

Key words: Endochondral ossification, Periodic tensile stress, Static tensile stress, Proliferation, Differentiation

中图分类号:

R783.5

王亚斐,王思涵,郭小凯,刘畅. 不同加载频率拉应力对髁突软骨细胞增殖分化的影响[J]. 口腔医学研究, 2015, 31(7): 662-665.

WANG Ya-fei, WANG Si-han, GUO Xiao-kai, et al. Effect of Different Frequency Tensile Stress on the Proliferation and Differentiation of Mandibular Condylar Chondrocytes[J]. Journal of Oral Science Research, 2015, 31(7): 662-665.

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