成牙本质细胞表达相关蛋白作用的研究进展

doi:10.13701/j.cnki.kqyxyj.2016.08.028

摘要/Abstract

摘要： 成牙本质细胞分泌的蛋白质、基质、生长因子等在牙齿发育和牙髓损伤修复中都起着重要的调节作用。牙本质基质蛋白(DMP1)、牙本质涎蛋白(DSP)、牙本质磷蛋白(DPP)、巢蛋白、clock蛋白、骨黏附蛋白聚糖(OSAD)等在牙本质形成、矿化及牙本质修复等过程中发挥着各自的作用。此外,其中某些蛋白对常染色体隐性低血磷性佝偻病、非矿化组织疾病发病机制的研究具有重要意义。本文就成牙本质细胞表达相关蛋白在牙发育及损伤修复中的作用机制做一综述。

关键词: 成牙本质细胞, 牙齿发育, 牙髓损伤修复

Abstract: Odontoblasts secreted proteins, matrix, and growth factors play important roles in regulating the tooth development and dental pulp damage repair. Dentin matrix protein (DMP1) and dentin sialoprotein (DSP), dentin phosphoprotein (DPP), nest protein, clock proteins, bone adhesion proteoglycans (OSAD) play important roles in the process of dentine formation, mineralization and dentine repair. In addition, some proteins will have important guiding significance for the pathogenesis research of autosomal recessive low blood phosphorus rickets and non mineralized tissue disease .This article aims to explain the role of odontoblasts related proteins in tooth development and damage repairation.

Key words: Odontoblast, Tooth development, Dental pulp damage repairation

中图分类号:

Q132

徐高祥,张琦,张鲁鲁,于士洋,姜秋,盖立婷. 成牙本质细胞表达相关蛋白作用的研究进展[J]. 口腔医学研究, 2016, 32(8): 886-889.

XU Gao-xiang, ZHANG Qi, ZHANG Lu-lu, DING Shi-yang, JIANG Qiu, GAI Li-ting. Research Progress of Odontoblasts Related Proteins in Tooth Development and Damage Repairation[J]. Journal of Oral Science Research, 2016, 32(8): 886-889.

参考文献

[1] Hao J, Ramachandran A, George A. Temporal and spatial localization of the dentin matrix proteins during dentin biomineralization [J]. Journal of Histochemistry & Cytochemistry, 2009, 57(3)∶227-237
[2] Suzuki S, Haruyama N, Nishimura F, et al. Dentin sialophosphoprotein and dentin matrix protein-1: two highly phosphorylated proteins in mineralized tissues [J]. Archives of oral biology, 2012, 57(9)∶1165-1175
[3] George A, Sabsay B, Simonian P A, et al. Characterization of a novel dentin matrix acidic phosphoprotein. Implications for induction of biomineralization [J]. Journal of Biological Chemistry, 1993, 268(17)∶12624-12630
[4] Atul Suresh Deshpande, Ping-An Fang, Xiaoyuan Zhang,et al. Primary Structure and Phosphorylation of Dentin Matrix Protein1 (DMP1) and Dentin Phosphophoryn (DPP) Uniquely Determine Their Role in Biomineralization [J]. Biomacromolecules, 2011, 12(8)∶2933-2945
[5] Beniash E, Deshpande A S, Fang P A, et al. Possible role of DMP1 in dentin mineralization [J]. Journal of structural biology, 2011, 174(1)∶100-106
[6] Martinez E F, Silva L A H, Furuse C, et al. Dentin matrix protein 1 (DMP1) expression in developing human teeth [J]. Brazilian dental journal, 2009, 20(5)∶365-369
[7] Sun Y, Lu Y, Chen L, et al. DMP1 processing is essential to dentin and jaw formation [J]. Journal of dental research, 2011, 90(5)∶619-624
[8] Rangiani A, Cao Z, Sun Y, et al. Protective roles of DMP1 in high phosphate homeostasis [J]. PloS one, 2012, 7(8)∶e42329
[9] Rangiani A, Cao Z G, Liu Y, et al. Dentin matrix protein 1 and phosphate homeostasis are critical for postnatal pulp, dentin and enamel formation [J]. International journal of oral science, 2013, 4(4)∶189-195
[10] Gibson M P, Zhu Q, Wang S, et al. The rescue of dentin matrix protein 1 (DMP1)-deficient tooth defects by the transgenic expression of dentin sialophosphoprotein (DSPP) indicates that DSPP is a downstream effector molecule of DMP1 in dentinogenesis [J]. Journal of Biological Chemistry, 2013, 288(10)∶7204-7214
[11] Siyam A, Wang S, Qin C, et al. Nuclear localization of DMP1 proteins suggests a role in intracellular signaling [J]. Biochemical and biophysical research communications, 2012, 424(3)∶641-646
[12] MacDougall M, Simmons D, Luan X, et al. Dentin phosphoprotein and dentin sialoprotein are cleavage products expressed from a single transcript coded by a gene on human chromosome 4 Dentin phosphoprotein DNA sequence determination [J]. Journal of Biological Chemistry, 1997, 272(2)∶835-842

[13] Napierala D, Sun Y, Maciejewska I, et al. Transcriptional repression of the Dspp gene leads to dentinogenesis imperfecta phenotype in Col1a1-Trps1 transgenic mice [J]. Journal of Bone and Mineral Research, 2012, 27(8)∶1735-1745
[14] Zhu Q, Gibson M P, Liu Q, et al. Proteolytic processing of dentin sialophosphoprotein is essential to dentinogenesis [J]. Journal of Biological Chemistry, 2012, 287(36)∶30426-30435
[15] 张蓉, 徐平西. 早期牙髓损伤修复中牙本质涎磷蛋白表达的免疫组化研究[J].牙体牙髓牙周病学杂志, 2001, 11(2)∶89-91
[16] Prasad M, Zhu Q, Sun Y, et al. Expression of dentin sialophosphoprotein in non-mineralized tissues [J]. Journal of Histochemistry & Cytochemistry, 2011, 59(11)∶1009-1021
[17] Butler W T, Bhown M, Brunn J C, et al. Isolation, characterization and immunolocalization of a 53-kDal dentin sialoprotein (DSP) [J]. Matrix, 1992, 12(5)∶343-351
[18] Yamakoshi Y. Dentinogenesis and dentin sialophosphoprotein (DSPP) [J]. Journal of Oral Biosciences, 2009, 51(3)∶134-142
[19] 张莹, 史俊南, 汪平, 等. 人牙本质涎蛋白在人牙胚发育过程中的表达和意义[J].牙体牙髓牙周病学杂志, 2004, 13(11)∶614-616
[20] Quispe-Salcedo A, Ida-Yonemochi H, Nakatomi M, et al. Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice [J]. Biomedical Research, 2012, 33(2)∶119-132
[21] Lee S Y, Kim S Y, Park S H, et al. Effects of recombinant dentin sialoprotein in dental pulp cells [J]. Journal of dental research, 2012, 91(4)∶407-412
[22] Prasad M, Butler W T, Qin C. Dentin sialophosphoprotein in biomineralization [J]. Connective tissue research, 2010, 51(5)∶404-417
[23] Suzuki S,Sreenath T,Haruyama N, et al. Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization [J].Matrix Biol, 2009, 4(2)∶221-229
[24] Kawamoto M, Ishiwata T, Cho K, et al. Nestin expression correlates with nerve and retroperitoneal tissue invasion in pancreatic cancer [J]. Human pathology, 2009, 40(2)∶189-198
[25] About I, Laurent-Maquin D, Lendahl U, et al. Nestin expression in embryonic and adult human teeth under normal and pathological conditions [J]. The American journal of pathology, 2000, 157(1)∶287-295
[26] Fujita S, Hideshima K, Ikeda T. Nestin expression in odontoblasts and odontogenic ectomesenchymal tissue of odontogenic tumours [J]. Journal of clinical pathology, 2006, 59(3)∶240-245
[27] Ishikawa Y, Ida-Yonemochi H, Nakakura-Ohshima K, et al. The relationship between cell proliferation and differentiation and mapping of putative dental pulp stem/progenitor cells during mouse molar development by chasing BrdU-labeling [J]. Cell and tissue research, 2012, 348(1)∶95-107
[28] Zheng L, Ehardt L, McAlpin B, et al. The tick tock of odontogenesis [J]. Experimental cell research, 2014, 325(2)∶83-89
[29] Zheng L, Papagerakis S, Schnell S D, et al. Expression of clock proteins in developing tooth [J]. Gene Expression Patterns, 2011, 11(3)∶202-206
[30] Wendel M, Sommarin Y, Heinegrd D. Bone matrix proteins: isolation and characterization of a novel cell-binding keratan sulfate proteoglycan (osteoadherin) from bovine bone [J]. The Journal of cell biology, 1998, 141(3)∶839-847
[31] Nikdin H, Olsson M L, Hultenby K, et al. Osteoadherin accumulates in the predentin towards the mineralization front in the developing tooth [J]. PloS one, 2012, 7(2)∶e31525