口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (4): 371-374.DOI: 10.13701/j.cnki.kqyxyj.2017.04.006

• 基础研究论著 • 上一篇    下一篇

骨皮质切开术通过促进成骨影响大鼠牙齿快速移动安全性的研究

朱绍跃1,2,3, 刘宗响2, 李晓明2, 刘浩3, 王鹏来1,2*   

  1. 1. 徐州医科大学附属徐州口腔医院种植中心 江苏 徐州 221002;
    2. 徐州医医科大学口腔医学院 江苏 徐州 221004;
    3. 徐州医科大学附属徐州口腔医院 江苏 徐州 221002
  • 收稿日期:2016-06-22 出版日期:2017-04-20 发布日期:2017-04-24
  • 通讯作者: 王鹏来,电话:18952175181
  • 作者简介:朱绍跃(1984~ ),男,江苏徐州人,主治医师,硕士,主要从事口腔内科的临床治疗工作。

Influence of Corticotomy on Safety of Orthodontic Tooth Movement via Promoting Osteogenesis in Rats.

ZHU Shao-yue1,2,3, LIU Zong-xiang2, LI Xiao-ming2,LIU Hao3, WANG Peng-lai1,2*.   

  1. 1. Dental Implant Center, Affiliated Xuzhou Stomatological Hospital, Xuzhou Medical College. Xuzhou 221002, China;
    2. School of Stomatology, Xuzhou Medical College. Xuzhou 221004, China;
    3. Affiliated Xuzhou Stomatological Hospital, Xuzhou Medical College. Xuzhou 221002, China.
  • Received:2016-06-22 Online:2017-04-20 Published:2017-04-24

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摘要： 目的:研究骨皮质切开术对大鼠正畸牙移动的影响及其组织学改建机制。方法:选用健康未孕雌性SD大鼠48只,随机分为骨皮质切开手术组和对照组。在手术组大鼠行骨皮质切开术,对照组大鼠行对照手术后,构建正畸牙移动模型。在牙移动0、1、3、7 d分别处死2组大鼠各6只。测量牙移动距离并制备组织学切片行HE染色、骨钙素及Runx2免疫组织化染色。采用SPSS16.0 软件包对数据进行统计分析,P<0.05为差异有显著性。结果:骨皮质切开组大鼠在移动3 d后牙移动速度大于假手术组大鼠(P<0.05)。牙移动第3天和第7天,手术组大鼠张力区新骨面积大于假手术大鼠(P<0.05),同时,此时手术组张力区骨钙素阳性成骨细胞增加(P<0.05)。手术组大鼠牙移动3 d及7 d时张力区Run2表达均高于假手术组(P<0.05)。结论:骨皮质切开术加速正畸牙移动的同时促进牙周组织成骨活性,这可能是保障牙移动安全性的关键因素。

Abstract: Objective: To study the influences of corticotomy on orthodontic tooth movement (OTM) and the underlying mechanism in rats. Methods: Forty-eight female Sprague-Dawley rats were randomly assigned to corticotomy group (Cort, 24 rats) and sham-corticotomy group (Sham, 24 rats). All rats were subjected OTM after corticotomy or sham surgery. Six rats of each group were executed on day 0, 1, 3 and 7 of OTM. OTM were measured with an electronic digital caliper. New-formed bone was counted on the intension side with HE staining. Osteocalcin and Runx2 were measured by IHC on the tension side. Data were analyzed with the SPSS software version 16.0 and P<0.05 was considered as statistically significant. Results: OTM on day 1 and 7 in the Cort group increased when compared to the sham group. New-formed bone on the tension side increased in the Cort group on day 3 and 7. Osteocalcin positive osteoblasts on the tension side also increased in the Cort group on day 3 and 7. Expression of Runx2 increased on the tension side in the Cort group on day 3 and 7. Conclusion: Corticotomy accelerated OTM in rats and the promoted bone formation may guarantee the safety of OTM in rats.

Key words: Corticotomy , Orthodontic tooth movement , Osteoblast Runx2

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