口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (8): 866-869.DOI: 10.13701/j.cnki.kqyxyj.2018.08.016

• 颞下颌关节与颌骨研究 • 上一篇    下一篇

Sonic hedgehog参与人胰岛素样生长因子-1信号通路促使下颌骨髁突过度生长的实验研究

陈宇翔1,2#, 黄群2#, 张武阳1, 马秦1*, 龙星3*   

  1. 1. 军事口腔医学国家重点实验室,解放军第四军医大学(空军军医大学)口腔医院颌面外科 陕西 西安 710032;
    2. 广东省妇幼保健院口腔医学中心 广东 广州 511400;
    3. 口腔生物医学教育部重点实验室,武汉大学口腔医院颌面外科 湖北 武汉 430079
  • 收稿日期:2018-02-26 出版日期:2018-08-28 发布日期:2018-08-23
  • 通讯作者: 马秦,E-mail: qinma@ fmmu.edu.cn;龙星,E-mail: longxing@whu.edu.cn;#为共同第一作者
  • 作者简介:陈宇翔(1982~ ),男,主治医师,博士,主要从事颞颌关节疾病的临床与基础研究。黄群(1967~ ),男,主任医师,博士,主要从事颞颌关节疾病及唇腭裂的临床与基础研究。
  • 基金资助:
    国家自然科学基金青年项目(编号:81400481)

Animal Experiment on Cooperation between Shh and IGF-1 in Promoting Mandibular Cartilage Overgrowth

CHEN Yu-xiang1,2#, HUANG Qun2#, ZHANG Wu-yang1, MA Qin1*, LONG Xing3*   

  1. 1. State Key Laboratory of Military Stomatology, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China;
    2. Stomatology Center, Guangdong Women and Children Hospital, Guangzhou 511400, China;
    3. Department of Oral and Maxillofacial Surgery, School of Stomatology, Wuhan University, Wuhan 430079, China
  • Received:2018-02-26 Online:2018-08-28 Published:2018-08-23

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摘要： 目的:通过活体动物实验观察人胰岛素样生长因子-1(insulin-like growth factor,IGF-1) 与Sonic hedgehogxinh (Shh)介导的信号通路促进了髁突过度生长的作用。方法:制备IGF-1高表达表型转基因小鼠模型。将转基因鼠随机分为为5组:Cyclopamine刺激组;NVP-AEW541刺激组;IGF-1刺激组;Cyclopamine+IGF-1刺激组;生理盐水刺激组:空白对照,每组3只。随机选取一侧关节腔自31 d起隔天连续进行上述药物注射,共14 d,注射结束后收获实验侧髁突,固定,比较两侧髁突大小,分析差异性。结果:Cyclopamine刺激组及生理盐水刺激组实验侧与对照侧髁突大小比较差异无统计学意义。NVP-AEW541刺激组、IGF-1刺激组以及Cyclopamine+ IGF-1刺激组实验侧髁突小于对照侧髁突(P<0.05)。结论:IGF-1介导的信号通路促进了髁突肥大髁突的过度生长。

关键词: 髁突肥大, 音猬因子, 人胰岛素样生长因子-1

Abstract: Objective: To investigate the combination effect of Shh and IGF-1 in promoting the mandible condylar cartilage overgrowth. Methods: Transgenic rats with IGF-1-over expression in condylar cartilage were constructed to allow the local administration of IGF-1 and its specific inhibitor NVP-AEW541, and Cyclopamine, the specific inhibitor of Shh. All rats were divided into five experiment groups by different stimulating additions :(1)Cyclopamine;(2)NVP-AEW541;(3)IGF-1;(4)Cyclopamine+ IGF-1;(5)physiological saline. Unilateral condyle was randomly chosen to make the intra-capsule injection, and then its size was compared to the control condyle. Results: Compared with control condyle, the sizes of the condyle which were local injected with NVP-AEW541, IGF-1, and Cyclopamine+ IGF-1 were significantly smaller (P<0.05). There was no difference on the size of the condyle between cyclopamine and physiological saline stimulation groups (P<0.05). Conclusion: IGF-1 promotes human TMJ cartilage overgrowth in the developing process of condylar hyperplasia.

Key words: Condylar hyperplasia, Sonic hedgehog, Insulin-like growth factor-1