后牙双斜面导板矫治器对大鼠髁突Ⅱ型胶原和VEGF表达的影响研究

doi:10.13701/j.cnki.kqyxyj.2019.04.010

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (4): 346-350.DOI: 10.13701/j.cnki.kqyxyj.2019.04.010

后牙双斜面导板矫治器对大鼠髁突Ⅱ型胶原和VEGF表达的影响研究

王硕¹, 华先明^1,2*, 孙雨虹³, 徐焰¹, 夏露露¹

1. 武汉大学口腔医学院口腔基础医学省部共建国家重点实验室培训基地和口腔生物医学教育部重点实验室湖北武汉 430079;
2. 武汉大学口腔医院正畸二科湖北武汉 430079;
3. 北京大学深圳医院口腔正畸科广东深圳 518035

收稿日期:2018-08-20 出版日期:2019-04-28 发布日期:2019-04-23
通讯作者: 华先明,E-mail:hxm@whu.edu.cn
作者简介:王硕(1991~ ),男,湖北荆州人,硕士,医师,主要从事口腔正畸学研究。
基金资助:
武汉市科技局应用基础研究计划;(编号:2015060101010052)

Effect of Twin Inclined Plane Appliance on Expression of Type Ⅱ Collagen and Vascular Endothelial Growth Factor in Condyle of Growing Rats

WANG Shuo¹, HUA Xian-ming^1,2*, SUN Yu-hong³, XU Yan¹, XIA Lu-lu¹

1. The State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China;
2. Department of Orthodontics, Hospital of Stomatology, Wuhan University, Wuhan 430079, China;
3. Department of Orthodontics, Peking University Shenzhen Hospital, Shenzhen 518035, China

Received:2018-08-20 Online:2019-04-28 Published:2019-04-23

摘要/Abstract

摘要： 目的: 探讨后牙双斜面导板矫治器(Twin Inclined Plane Appliance,TIPA)引导生长期大鼠下颌后退时,髁突软骨Ⅱ型胶原和VEGF的表达。方法: 将48只6周龄雄性Wistar大鼠随机分为实验组和对照组,实验组戴TIPA,对照组不做任何处理,分别在3 d、14 d、30 d和60 d处死大鼠。免疫组化检测Ⅱ型胶原和VEGF在髁突软骨后区中表达的变化。结果: 实验组与对照组相比,Ⅱ型胶原在第30天表达明显减少,在第60天时最低,均有显著性差异(P＜0.01);实验组VEGF在第30天表达增强(P＜0.05),第60天时增强幅度最大(P＜0.01)。结论: Ⅱ型胶原和VEGF参与了TIPA引导生长期大鼠下颌“渐进式”后退时的髁突软骨的改建。

关键词: 后牙双斜面导板矫治器, Ⅱ型胶原, 血管内皮因子

Abstract: Objective: To investigate the effect of twin inclined plane appliance (TIPA) on the expression of type II collagen and vascular endothelial growth factor (VEGF) in condyle of growing rats. Methods: Male 6-week-old Wistar rats (n=48) were randomly divided into control group (n=16) and experimental group (n=32). Rats in experiment group wore TIPA, whereas for the control groups no appliances were fitted. After 3, 14, 30, and 60 days, the rats were euthanatized and samples were collected. Immuohistochemical method was used to localize and examine the type II collagen and VEGF in the posterior area of mandibular condylar cartilage (MCC). Results: The expression of type II collagen decreased significantly on day 30 and reached the lowest on day 60 in the experimental group (P<0.01). The expression of VEGF increased significantly on day 30 (P<0.05) and reached the peak on day 60 in the experimental group (P<0.01). Conclusion: Both type II collagen and VEGF take part in MCC remolding during the gradually induced backward movement of mandible induced by TIPA.

Key words: Twin inclined plane appliance, Type Ⅱ collagen, Vascular endothelial growth factor

王硕, 华先明, 孙雨虹, 徐焰, 夏露露. 后牙双斜面导板矫治器对大鼠髁突Ⅱ型胶原和VEGF表达的影响研究[J]. 口腔医学研究, 2019, 35(4): 346-350.

WANG Shuo, HUA Xian-ming, SUN Yu-hong, XU Yan, XIA Lu-lu. Effect of Twin Inclined Plane Appliance on Expression of Type Ⅱ Collagen and Vascular Endothelial Growth Factor in Condyle of Growing Rats[J]. Journal of Oral Science Research, 2019, 35(4): 346-350.

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后牙双斜面导板矫治器对大鼠髁突Ⅱ型胶原和VEGF表达的影响研究

Effect of Twin Inclined Plane Appliance on Expression of Type Ⅱ Collagen and Vascular Endothelial Growth Factor in Condyle of Growing Rats

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