ProgranuIin衍生工程蛋白Atsttrin对牙周膜干细胞成骨分化作用的影响

doi:10.13701/j.cnki.kqyxyj.2019.07.007

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (7): 643-646.DOI: 10.13701/j.cnki.kqyxyj.2019.07.007

ProgranuIin衍生工程蛋白Atsttrin对牙周膜干细胞成骨分化作用的影响

于淼^1*, 李倜¹, 白建文¹, 王丽美², 段晓琪³, 孙龙⁴, 孙钦峰³

1. 山东省潍坊市人民医院口腔科山东潍坊 261000;
2. 山东大学齐鲁医院牙周科山东济南 250000;
3. 山东大学口腔医学院牙周科山东济南 250000;
4. 山东省滕州市中心医院牙周科山东枣庄 277100

收稿日期:2018-11-13 出版日期:2019-07-25 发布日期:2019-07-24
通讯作者: 于淼,E-mail:dentistyum@163.com
作者简介:于淼(1982~ ),女,博士,辽宁人,研究方向:牙周病学,口腔微生物学
基金资助:
山东省医药卫生科技发展计划项目(编号:2017WS732)

Effects of Progranulin Derived Engineered Protein Atsttrin on Osteogenic Differentiation of Periodontal Ligament Stem Cells

YU Miao^1*, LI Ti¹, BAI Jianwen¹, WANG Limei², DUAN Xiaoqi³, SUN Long⁴, SUN Qinfeng³

1. Department of Stomatology, Weifang People's Hospital, Weifang 261000, China;
2. Department of Periodontology, Qilu Hospital, Institute of Stomatology, Shandong University, Jinan 250000, China;
3. Department of Periodontology, School of Stomatology, Shandong University, Jinan 250000, China;
4. Department of Stomatology, Tengzhou Central Hospital, Zaozhuang 277100, China

Received:2018-11-13 Online:2019-07-25 Published:2019-07-24

摘要/Abstract

摘要： 目的:探讨Atsttrin对牙周膜干细胞(periodontal ligament stem cells,PDLSCs)成骨分化的促进作用及Atsttrin逆转肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)对PDLSCs成骨分化的抑制作用。方法:体外分离培养PDLSCs,实验组分别加入25 μg/L Atsttrin、10 μg/L TNF-α、10 μg/L TNF-α+25 μg/L Atsttrin,检测PDLSCs的碱性磷酸酶(alkaline phosphatase activity,ALP)活性及成骨相关标志物ALP、runt相关转录因子-2(runt-related transcription factor-2,Runx2)的mRNA及蛋白表达水平;21 d时,进行茜素红染色和钙含量测定。结果:Atsttrin增强PDLSCs的ALP活性,增强ALP、Runx2基因及蛋白表达水平,促进矿化结节的形成。在各时间点中,25 μg/L Atsttrin组促进PDLSCs成骨分化及矿化的能力最强。结论:Atsttrin能促进PDLSCs的成骨分化及矿化,能拮抗TNF-α对PDLSCs成骨分化的抑制作用

关键词: 牙周膜干细胞, 细胞分化, Atsttrin

Abstract: Objective: To explore the positive effect of Atsttrin on osteogenic differentiation of PDLSCs and the reversing effect of Atsttrin on TNF-α-inhibited PDLSCs osteogenic differentiation. Methods: PDLSCs were obtained and treated with 25 μg/L Atsttrin, 10 μg/L TNF-α, and 10 μg/L TNF-α + 25 μg/L Atsttrin, respectively. Alkaline phosphatase activity (ALP) and gene/protein expression levels of ALP and runt-related transcription factor-2 (Runx2) were assessed. The formation of mineralized nodules and determination of calcium content was observed. Results: Atsttrin enhanced the ALP activity and osteogenesis gene/protein expressions of PDLSCs, and promoted the formation of mineralized nodules. At three time points, the highest osteogenic differentiation and mineralization ability of PDLSCs were found in the 25 μg/L Atsttrin group. Conclusion: Atsttrin can directly promote the osteogenic differentiation and mineralization of PDLSCs, and can reverse the inhibitory effect of TNF-α on osteogenic differentiation of PDLSCs.

Key words: periodontal, ligament stem cells, cell differentiation , atsttrin

于淼, 李倜, 白建文, 王丽美, 段晓琪, 孙龙, 孙钦峰. ProgranuIin衍生工程蛋白Atsttrin对牙周膜干细胞成骨分化作用的影响[J]. 口腔医学研究, 2019, 35(7): 643-646.

YU Miao, LI Ti, BAI Jianwen, WANG Limei, DUAN Xiaoqi, SUN Long, SUN Qinfeng. Effects of Progranulin Derived Engineered Protein Atsttrin on Osteogenic Differentiation of Periodontal Ligament Stem Cells[J]. Journal of Oral Science Research, 2019, 35(7): 643-646.

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ProgranuIin衍生工程蛋白Atsttrin对牙周膜干细胞成骨分化作用的影响

Effects of Progranulin Derived Engineered Protein Atsttrin on Osteogenic Differentiation of Periodontal Ligament Stem Cells

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