基质金属蛋白酶-9对出生后小鼠骨发育和骨改建的作用研究

doi:10.13701/j.cnki.kqyxyj.2019.07.021

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (7): 708-711.DOI: 10.13701/j.cnki.kqyxyj.2019.07.021

基质金属蛋白酶-9对出生后小鼠骨发育和骨改建的作用研究

柴纪华, 栗钰晴, 袁国华, 张玉峰^*

口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室, 武汉大学口腔医学院湖北武汉 430079

收稿日期:2019-01-22 出版日期:2019-07-25 发布日期:2019-07-24
通讯作者: 张玉峰,E-mail:zyf@whu.edu.cn
作者简介:柴纪华(1992~ ),女,山东聊城人,硕士在读,主要从事种植学和发育学相关的研究工作

Effects of Matrix Metalloproteinase-9 on Bone Development and Remodeling of Postnatal Mice

CHAI Jihua, LI Yuqing, YUAN Guohua, ZHANG Yufeng^*

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-Most & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China.

Received:2019-01-22 Online:2019-07-25 Published:2019-07-24

摘要/Abstract

摘要： 目的:本研究旨在探讨基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)在出生后小鼠股骨与颅面骨发育与改建过程中的作用。方法:使用X射线投射技术、Micro-CT扫描及三维成像技术、组织学染色技术,对比分析MMP-9基因敲除(MMP-9^-/-)小鼠与野生型小鼠在不同发育阶段骨组织的特征。结果:较之野生型小鼠,MMP-9^-/-小鼠的股骨骨小梁厚度明显低于野生型小鼠。12周MMP-9^-/-小鼠的骨髓腔中存在大量脂肪细胞。MMP-9^-/-小鼠颅面骨矿化程度低于野生型小鼠。结论:MMP-9基因缺失抑制了小鼠股骨与颅面骨的发育与改建,提示MMP-9在骨发育与骨稳态过程中发挥重要作用

关键词: 基质金属蛋白酶-9, 股骨, 颅面骨, 发育与改建

Abstract: Objective: To investigate the effect of matrix metalloproteinase-9 (MMP-9) on the development and remodeling of femur and craniofacial bone in postnatal mice. Methods: X-ray projection, Micro-CT scanning, three-dimensional imaging, and histological staining were used to observe and analyze the characteristics of bone of MMP-9^-/- mice with wild-type mice at different developmental stages. Results: Compared with the wild-type mice, the femoral trabecular thickness of adult mice was lower than that of wild-type mice. Fat cells were abundant in the bone marrow cavity at 12-week-old MMP-9^-/- mice. The degree of skull mineral density in MMP-9^-/- mice was lower than that of normal mice. Conclusion: MMP-9 gene deletion inhibited the development and remodeling of femoral and craniofacial bones in mice, suggesting that MMP-9 plays an important role in bone development and bone homeostasis.

Key words: matrix metalloproteinase-9, femur, craniofacial bone , development and remodeling

柴纪华, 栗钰晴, 袁国华, 张玉峰. 基质金属蛋白酶-9对出生后小鼠骨发育和骨改建的作用研究[J]. 口腔医学研究, 2019, 35(7): 708-711.

CHAI Jihua, LI Yuqing, YUAN Guohua, ZHANG Yufeng. Effects of Matrix Metalloproteinase-9 on Bone Development and Remodeling of Postnatal Mice[J]. Journal of Oral Science Research, 2019, 35(7): 708-711.

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基质金属蛋白酶-9对出生后小鼠骨发育和骨改建的作用研究

Effects of Matrix Metalloproteinase-9 on Bone Development and Remodeling of Postnatal Mice

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