RNA可变剪接调控因子核不均一核糖核蛋白F在头颈鳞状细胞癌中的表达和功能

doi:10.13701/j.cnki.kqyxyj.2019.12.007

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (12): 1129-1131.DOI: 10.13701/j.cnki.kqyxyj.2019.12.007

RNA可变剪接调控因子核不均一核糖核蛋白F在头颈鳞状细胞癌中的表达和功能

熊英杰, 贾荣^*

武汉大学口腔医学院,湖北省口腔基础医学重点实验室-省部共建国家重点实验室培育基地口腔生物医学教育部重点实验室(武汉大学) 湖北武汉 430079

收稿日期:2019-06-05 出版日期:2019-12-28 发布日期:2019-12-23
通讯作者: 贾荣,E-mail:jiarongwh@whu.eju.cn
作者简介:熊英杰(1994~ ),女,重庆人,硕士在读,主要从事口腔癌前病变和牙体牙髓病学相关基础研究和临床工作。
基金资助:
国家自然科学基金(编号:81470741)

Expression and Function of Splicing Factor hnRNP F in Head and Neck Squamous Cell Carcinoma

XIONG Yingjie, JIA Rong^*

Hubei-MOST KLOS & KLOBME, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.

Received:2019-06-05 Online:2019-12-28 Published:2019-12-23

摘要/Abstract

摘要： 目的:研究RNA可变剪接调控因子核不均一核糖核蛋白F(heterogeneous nuclear ribonucleoprotein F,hnRNP F) 在头颈鳞状细胞癌中的表达和功能。方法:分析TCGA数据库和Oncomine数据库头颈鳞状细胞癌中hnRNP F的表达数据。用在线cibioportal数据库分析hnRNP F表达与头颈鳞状细胞癌患者无病生存率的关系。用小干扰RNA (small interfering RNA,siRNA)抑制CAL-27细胞的hnRNP F的表达,分析细胞生长。结果:TCGA和Oncomine数据分析结果显示hnRNP F在头颈鳞状细胞癌中表达水平显著升高,hnRNP F表达水平高的头颈鳞状细胞癌患者的无病生存率显著低于hnRNP F表达水平低的患者。沉默hnRNP F的表达显著抑制了CAL-27细胞的生长。结论:hnRNP F在头颈鳞状细胞癌的发生发展中可能有重要作用。

关键词: 头颈鳞状细胞癌, 核不均一核糖核蛋白F, RNA剪接

Abstract: Objective: To investigate the expression and function of hnRNP F in head and neck squamous cell carcinoma (HNSCC). Methods: The expression of hnRNP F in head and neck squamous cell carcinoma was analyzed in TCGA and Oncomine database. The relationship between hnRNP F expression and disease-free survival in HNSCC was analyzed in cbioportal database. CAL-27 cells were transfected with anti-hnRNP F siRNA or non-specific siRNA. The growth curve of cells was analyzed by cell counting. Results: HnRNP F was overexpressed in HNSCC patients. Patients with high expression of hnRNP F showed significantly poorer disease-free survival than those with low expression of hnRNP F. Knockdown of hnRNP F significantly inhibited CAL-27 cell growth. Conclusion: hnRNP F may play important roles in the development of HNSCC.

Key words: head and neck squamous cell carcinoma, hnRNP F, RNA splicing

熊英杰, 贾荣. RNA可变剪接调控因子核不均一核糖核蛋白F在头颈鳞状细胞癌中的表达和功能[J]. 口腔医学研究, 2019, 35(12): 1129-1131.

XIONG Yingjie, JIA Rong. Expression and Function of Splicing Factor hnRNP F in Head and Neck Squamous Cell Carcinoma[J]. Journal of Oral Science Research, 2019, 35(12): 1129-1131.

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RNA可变剪接调控因子核不均一核糖核蛋白F在头颈鳞状细胞癌中的表达和功能

Expression and Function of Splicing Factor hnRNP F in Head and Neck Squamous Cell Carcinoma

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