口腔医学研究

口腔医学研究 ›› 2025, Vol. 41 ›› Issue (11): 993-997.DOI: 10.13701/j.cnki.kqyxyj.2025.11.012

• 口腔正畸学研究 • 上一篇    下一篇

酪蛋白磷酸多肽水合硅酸钙促进邻面去釉后牙釉质再矿化的体外研究

梁远帆1, 邓文振2, 王紫仪1, 侯晓璐1, 刘畅3, 张丽萍4, 梁永强1*   

  1. 1.华北理工大学口腔医学院 河北 唐山 063210;
    2.菏泽医学专科学校附属医院 山东 菏泽 274000;
    3.天津市口腔医院种植科 南开大学医学院 天津 300041;
    4.华北理工大学附属医院口腔科 河北 唐山 063015
  • 收稿日期:2025-03-27 出版日期:2025-11-28 发布日期:2025-11-25
  • 通讯作者: *梁永强,E-mail:278840795@qq.com
  • 作者简介:梁远帆 (1998~ ),女,陕西铜川人,硕士在读,主要从事口腔正畸和牙体牙髓病相关研究。

Promoting of Enamel Remineralization by CPP-CSH After Interproximal Enamel Reduction: An In Vitro Study

LIANG Yuanfan1, DENG Wenzhen2, WANG Ziyi1, HOU Xiaolu1, LIU Chang3, ZHANG Liping4, LIANG Yongqiang1*   

  1. 1. College of Stomatology, North China University of Science and Technology, Tangshan 063210, China;
    2. Affiliated Hospital of Heze Medical College, Heze 274000, China;
    3. Department of Implantology, Tianjin Stomatological Hospital, School of Medicine, Nankai University, Tianjin 300041, China;
    4. Department of Stomatology, Affiliated Hospital of North China University of Science and Technology, Tangshan 063015, China
  • Received:2025-03-27 Online:2025-11-28 Published:2025-11-25

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摘要： 目的: 探讨酪蛋白磷酸多肽-水合硅酸钙(casein phosphopeptide-calcium silicate hydrate, CPP-CSH)对邻面去釉后牙釉质再矿化的促进作用,评估其作为防龋材料的可行性。方法: 制备1%、3%、5%浓度的CPP-CSH溶液,建立离体牙邻面去釉模型。将不同浓度的CPP-CSH溶液作用于邻面去釉牙面,通过pH循环实验模拟口腔环境。通过能谱分析、显微硬度测试及扫描电镜评估再矿化效果,筛选最佳浓度并与10% CSH、多乐氟及护牙素对比。结果: 1%CPP-CSH组钙磷比(Ca/P)高于空白对照组(P<0.05),显微硬度提升(P<0.05),扫描电镜显示釉质表面划痕最少、矿化颗粒均匀沉积。与其他试剂相比,1% CPP-CSH的再矿化效果优于10%CSH(P<0.05),并与多乐氟相当(P>0.05)。结论: 1%CPP-CSH可有效促进邻面去釉后牙釉质再矿化,其效能优于10%CSH且与多乐氟相近,为正畸治疗中邻面去釉后的防龋提供了新型候选材料。

关键词: 邻面去釉, 酪蛋白磷酸多肽-水合硅酸钙, 牙釉质再矿化, 防龋

Abstract: Objective: To investigate the remineralization effect of casein phosphopeptide-calcium silicate hydrate (CPP-CSH) on enamel after interproximal enamel reduction (IER) and evaluate its potential as a safe caries-preventive material. Methods: CPP-CSH solutions at 1%, 3%, and 5% concentrations were prepared. Different concentrations of CPP-CSH solutions were applied to interproximal enamel-reduced tooth surfaces. The oral environment was simulated through pH cycling experiments. The remineralization efficacy was evaluated via energy-dispersive spectroscopy analysis, microhardness testing, and scanning electron microscopy (SEM) to screen the optimal concentration, which was then compared with 10% CSH, Duraphat, and Tooth Mousse. Results: The 1% CPP-CSH group showed the highest Ca/P ratio (P<0.05), significant microhardness improvement (P<0.05), and the smoothest enamel surface with minimal scratches under SEM. The 1% CPP-CSH outperformed 10% CSH (P<0.05) and demonstrated comparable efficacy to Duraphat (P>0.05). Conclusion: 1% CPP-CSH effectively promotes enamel remineralization after IER, offering an alternative to fluoride-based agents and a novel clinical option for post-IER caries prevention.

Key words: interproximal enamel reduction, casein phosphopeptide-calcium silicate hydrate, enamel remineralization, caries prevention