茜素红与阿利新蓝双染色法对小鼠下颌骨发育的整体观察

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (1): 15-17.

茜素红与阿利新蓝双染色法对小鼠下颌骨发育的整体观察

赵一松¹,钟慧敏¹,何志伟²,张国梁^3*

1. 天津市滨海新区塘沽口腔医院颌面外科天津 300450;
2. 哈尔滨医科大学附属第二临床医学院;
3. 佳木斯大学附属口腔医院

收稿日期:2014-04-02 出版日期:2015-01-28 发布日期:2016-04-29
通讯作者: 张国梁,E-mail: 379378@qq.com
作者简介:赵一松(1979~ )男,山东东阿人,主治医师,硕士,主要从事口腔正畸的临床治疗工作。

Alizarin red and Alcian Blue Double Staining for the Whole Observation of Development of Mouse Mandible.

ZHAO Yi-song, ZHONG Hui-min, HE Zhi-wei, et al

Tanggu Stomatology Hospital of TianJin, Tianjin 300450

Received:2014-04-02 Online:2015-01-28 Published:2016-04-29

摘要/Abstract

摘要： 目的:探索用茜素红/阿利新蓝双染色法观察小鼠下颌骨的整体骨架发育。方法:应用不同条件的茜素红/阿利新蓝染色液对E15.5、E16.5、 E18.5和P90的小鼠进行整体骨架双染色,观察小鼠下颌骨的整体发育情况,重点关注Meckel's软骨和髁突软骨发育。结果:骨和软骨能被分别染成红色和蓝色,在体视显微镜下可清楚地观察到下颌骨的整体发育形态。Meckel's软骨与周围的骨组织在组织学上和空间上无连续性。髁突软骨独立于Meckel's软骨,与下颌骨骨膜紧密相连。结论:应用不同条件的茜素红/阿利新蓝整体骨架双染色法成功观察了小鼠下颌骨的整体发育过程。Meckel's软骨可能不直接参与下颌骨的骨化,髁突可能是来源于下颌骨末端骨膜间质细胞。[关键词] 阿利新蓝茜素红双染色法下颌骨发育整体观察

关键词: 阿利新蓝, 茜素红, 双染色法, 下颌骨发育, 整体观察

Abstract: Objective: To explore the feasibility of using the alizarin red/ alcian blue double staining to observe the whole development of mouse mandible. Methods: Alcian blue and alizarin red solution were used to double stain the E15.5, E16.5, E18.5 and P90 mouse mandibular skeleton. We observed the whole development of mandible, especially focused on the Meckel's cartilage and condylar cartilage. Results: The bone and cartilage could be stained as red or blue respectively. The morphology of the developmental mandible could be clearly observed under the stereo microscope. The Meckel's cartilage was not tightly connected to the surrounding tissues. The condylar cartilage was independent of the Meckel's cartilage, and was tightly connected to the mandibular periosteum. Conclusion: Using the alizarin red/alcian blue double staining could successfully observe the whole development process of mouse mandible. The Meckel's cartilage may not directly take part in the bone formation of mandible, and the condylar cartilage may originate from the mesenchymal cell in tail end of mandible.

Key words: Alcian blue, Alizarin red , Double staining, Mandibuloar development, Whole observation

中图分类号:

R782.2

赵一松,钟慧敏,何志伟,张国梁. 茜素红与阿利新蓝双染色法对小鼠下颌骨发育的整体观察[J]. 口腔医学研究, 2015, 31(1): 15-17.

ZHAO Yi-song, ZHONG Hui-min, HE Zhi-wei, et al. Alizarin red and Alcian Blue Double Staining for the Whole Observation of Development of Mouse Mandible.[J]. Journal of Oral Science Research, 2015, 31(1): 15-17.

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