BMP-2、bFGF基因转染的大鼠骨髓干细胞与牙胚细胞在体外混合培养对成牙基因的影响

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (1): 18-22.

BMP-2、bFGF基因转染的大鼠骨髓干细胞与牙胚细胞在体外混合培养对成牙基因的影响

邬薇薇,胡杨,何惠宇^*,韩祥祯,王欢欢

新疆医科大学第一附属医院口腔修复科新疆乌鲁木齐 830054

收稿日期:2014-11-14 出版日期:2015-01-28 发布日期:2016-04-29
通讯作者: 何惠宇,E-mail:hehuiyu01@126.com
作者简介:邬薇薇(1988~ ),女,浙江宁海县人,在读硕士,主要从事口腔修复学临床及基础的研究工作。
基金资助:
新疆维吾尔自治区科技厅科技支疆项目(编号:201291173)

Effect of co-culture of BMP-2 and bFGF Transfected Rat Bone Marrow Stem Cells and Germ Cells on Tooth Gene.

WU Wei-wei, HU Yang, HE Hui-yu, et al

Department of Prosthodontics, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054

Received:2014-11-14 Online:2015-01-28 Published:2016-04-29

摘要/Abstract

摘要： 目的:观察骨形态发生蛋白-2和(或)碱性成纤维细胞生长因子转染的大鼠骨髓干细胞(BMSCs)和牙胚细胞在体外混合培养对牙本质基质蛋白1、成釉蛋白、Ⅰ型胶原蛋白、同源异型盒基因1的影响。方法:采用实时定量PCR和Western blot的方法,检测 BMP-2和(或)bFGF对成牙基因的影响。结果:对照组、bFGF组、BMP-2组、bFGF/BMP-2组四组间,AMBN、COLLAGEN-Ⅰ、DLX1、DMP1基因在mRNA和蛋白水平表达量上存在明显差异(P<0.05)。结论:BMP2和bFGF有一定的协同交互作用,BMP2/bFGF/BMSCs/牙胚细胞可以作为构建组织工程牙的种子细胞。

关键词: 骨形态发生蛋白-2, 碱性成纤维细胞生长因子, 牙本质基质蛋白1, 成釉蛋白, Ⅰ型胶原蛋白同源异型盒基因1

Abstract: Objective: To observe the bone morphogenetic protein -2 (BMP-2) and (or) basic fibroblast growth factor (bFGF) transfected rat bone marrow stem cells (BMSCs) and germ mixed culture cells in vitro effects on dentin matrix protein 1 (DMP1), ameloblastin (AMBN), Ⅰ collagen (collagen-Ⅰ), homeobox gene 1 (DLX1)'s. Methods: From gene expression and protein levels by real-time quantitative PCR and Western blot to determine the level of BMP-2 and (or) the impact of bFGF on four genes. Results: The control group, bFGF group, BMP-2 group and bFGF / BMP-2 group among the four groups, AMBN, COLLAGEN-Ⅰ, DLX1, DMP1 genes were significantly different (P<0.05) in the expression of mRNA and protein levels. Conclusion: BMP2 and bFGF have synergistic interaction, BMP2 / bFGF / BMSCs / germ cells can be constructed as seed cells for tissue engineering of teeth.

Key words: Bone morphogenetic protein-2 , Basic fibroblast growth factor, Dentin matrix protein 1, Ameloblastin Ⅰ collagen , Homeobox gene 1

中图分类号:

Q132

邬薇薇,胡杨,何惠宇,韩祥祯,王欢欢. BMP-2、bFGF基因转染的大鼠骨髓干细胞与牙胚细胞在体外混合培养对成牙基因的影响[J]. 口腔医学研究, 2015, 31(1): 18-22.

WU Wei-wei, HU Yang, HE Hui-yu, et al. Effect of co-culture of BMP-2 and bFGF Transfected Rat Bone Marrow Stem Cells and Germ Cells on Tooth Gene.[J]. Journal of Oral Science Research, 2015, 31(1): 18-22.

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