口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (10): 969-973.

• 基础研究论著 • 上一篇    下一篇

脂肪来源干细胞膜片与支持材料结合能力的初步探讨

李芳菲1,刘娜2,杨曦1,兰贵华1,邓蔓菁1*   

  1. 1. 第三军医大学大坪医院野战外科研究所口腔科 重庆 400042;
    2. 解放军总医院口腔医学研究所 北京 100853
  • 收稿日期:2015-04-28 出版日期:2015-10-28 发布日期:2016-05-04
  • 通讯作者: 邓蔓菁,电话:023-68757575
  • 作者简介:李芳菲(1986~ ),女,河北人,硕士在读,主要从事牙周骨缺损修复的研究工作。
  • 基金资助:
    国家重点基础研究发展计划(2011CB964701);重庆市自然基金资助项目(CSTC2011BA5013)

Adhesion and Proliferation of Adipose Derived Stem Cells Sheet on Support Material

LI Fang-fei1, LIU Na2, YANG Xi1, LAN Gui-hua1, DENG Man-jing1*   

  1. 1. Department of Stomatology, Research Institute of Surgery, Daping Hospital, The Third Military Medical University, Chongqing 400042, China. 2. Institute of Stomatology, General Hospital of PLA, Beijing 100853, China
  • Received:2015-04-28 Online:2015-10-28 Published:2016-05-04

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摘要： 目的:探讨大鼠脂肪来源干细胞体外构建细胞膜片方法,并分析其同牙本质磨片和CBB(ceramic bovine bone,CBB)颗粒的结合能力和增殖影响。方法:从出生7 d大鼠体内提取脂肪组织并获得纯化的脂肪来源干细胞(ADSCs,adipose-derived mesenchymal stem cell), 采用四唑盐(MTT)比色法检测ADSCs增殖能力的变化;ADSCs经多向诱导分化后检测其向成骨细胞、脂肪细胞、成软骨细胞分化能力;ADSCs体外构建细胞膜片后,电镜下观察ADSCs膜片与CBB颗粒和牙本质磨片的结合能力,MTT比色法检测ADSCs和ADSCs膜片在材料上的增殖能力。结果:纯化的ADSCs具有良好的增殖能力,经体外诱导培养后向成骨细胞、脂肪细胞、成软骨细胞分化,电镜结果表明ADSCs膜片与牙本质磨片结合能力优于CBB颗粒,ADSCs膜片在牙本质磨片上的增殖能力优于CBB颗粒。结论:体外培养的大鼠脂肪来源干细胞具有种子细胞的特性,体外构建细胞膜片后与牙本质磨片的结合能力和增殖能力高于CBB颗粒,为细胞膜片治疗牙周缺损奠定基础。

关键词: 脂肪来源干细胞, 细胞膜片, 牙周组织再生

Abstract: Objective: To construct cells sheet from rat adipose derived mesenchymal stem cells (ADSCs), and investigate its adhesion and proliferation on dentin disc and ceramic bovine bone (CBB). Methods: ADSCs were extracted from adipose tissue of mice at 7 days of age. MTT was used to determine the proliferation capacity of ADSCs and ADSCs sheet on CBB and dentin disc. Multilineage differentiation of ADSCs into osteoblasts, adipocytes, and chondrocytes was also detected. The adhesion ability of ADSCs on CBB and dentin discs was examined by scanning electron microscope (SEM). Results: Purified ADSCs cells had excellent proliferation capacity. They could differentiate into osteoblasts, adipocytes, and chondrocytes. SEM results showed that ADSCs sheet had better adhesion on dentin disc than on CBB paticles. Conclusion: ADSCs sheet has higher proliferation and adhesion ability on dentin disc than CBB particles, which lays the foundation for cells sheet to treat periodontal defects.

Key words: Adipose derived stem cells, Cells sheet, Periodontal tissue regeneration, Dentin disc

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