口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (6): 584-587.

• 基础研究论著 • 上一篇    下一篇

大鼠咬肌H反射的双侧三叉神经运动核和咬肌电活动联合分析

余兰芳1,2, 王云2*, 汪萌芽1*   

  1. 1. 皖南医学院细胞电生理研究室 安徽 芜湖 241002;
    2. 皖南医学院 口腔医学院
  • 收稿日期:2014-10-30 出版日期:2015-06-28 发布日期:2016-04-29
  • 通讯作者: 王云,E-mail:whwy303@hotmail.com;;汪萌芽,E-mail:wangmy@wnmc.edu.cn
  • 作者简介:余兰芳(1989~ ),女,湖北宜昌人,硕士在读,主要从事口腔生理的基础研究工作。
  • 基金资助:
    国家自然科学基金(31271155);皖南医学院重点科研项目培育基金(wk2013Z09);芜湖市科技计划项目(2012cxy16)

Simultaneous Analyses of Electrical Activities in Bilateral Trigeminal Motor Nucleus and Masseter Muscle during Masseter H-reflex in Rats

YU Lan-fang, WANG Yun, WANG Meng-ya.   

  1. Cell Electrophysiology Laboratory, Wannan Medical College, Wuhu 241002
  • Received:2014-10-30 Online:2015-06-28 Published:2016-04-29

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被引次数

1

摘要： 目的:建立同步检测双侧三叉神经运动核(trigeminal motor nucleus, Mo5)电活动和咬肌电活动同步记录技术,为进一步分析咬肌H反射的神经通路和可塑性机制奠定基础。方法:取成年Wistar大鼠5只,在麻醉状态下进行双侧Mo5区电活动的同步记录,同时监测双侧咬肌肌电(EMG)等多项生理指标,并对给予咬肌神经电刺激时同步记录到的肌电反应进行分析。结果:①记录稳定信号30 min,可见Mo5区神经元呈一低频放电模式:左侧(1.25±0.09) Hz,右侧(1.20±0.39) Hz,P>0.05,咬肌未见可测的电活动;②给予单侧咬肌神经电刺激(0.1~20 mA,200 μs,0.3 Hz)不仅诱导出同侧咬肌H反射,也诱导出对侧咬肌H反射;③给予单侧咬肌神经电刺激诱发H反射时,同时引发同侧和对侧Mo5核团生物电反应;④刺激侧咬肌EMG幅值明显高于对侧(P<0.05);刺激侧Mo5核团生物电幅值与对侧相比较差异无统计学意义。结论:对大鼠咬肌H反射的双侧Mo5区和咬肌电活动可进行有效的同步记录和分析,可用于咬肌H反射的神经通路和可塑性机制研究。

关键词: 三叉神经运动核, H反射, 细胞外记录

Abstract: Objective: To analyze the neural pathways and their underlying mechanisms of H-reflex in masseter muscles, the simultaneous recording techniques of electrical activities in bilateral trigeminal motor nucleus (Mo5) and masseter muscle during H-reflex were established in rats. Methods: Adult Wistar rats (n=5) were anesthetized and bilateral Mo5 were chosen to conduct extracellular recordings. Bilateral electromyography (EMG) of masseter muscle and other physiological functions were simultaneously monitored by a polygraph. The responses evoked by electrical stimulation of masseter nerve were preliminarily analyzed. Results: During the baseline recording for 30 minutes, a low-frequency discharge pattern was observed in bilateral Mo5 (Left: 1.25±0.09 Hz, Right: 1.20±0.39 Hz, P>0.05), but masseter EMG did not reveal any measurable activity. Not only ipsilateral masseter H-reflex but also contralateral masseter H-reflex were induced simultaneously by giving unilateral masseter nerve electrical stimulation. Moreover, responses of ipsilateral and contralateral Mo5 were evoked during the masseter H-reflex. The response amplitude of ipsilateral masseter EMG was higher than that of contralateral one (P<0.05), but no significant difference between ipsilateral and contralateral Mo5 was observed (P>0.05). Conclusion: It is feasible to analyze the electrical activities in bilateral Mo5 and masseter muscle during masseter H-reflex. This may serve as an appropriate approach to exploring the neural pathways and plasticity mechanisms of H-reflex in masseter muscles.

Key words: Trigeminal motor nucleus , H-reflex , Extracellular recording

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