PEN载体传递ODN对人成骨样细胞细胞周期与成骨分化的影响

doi:10.13701/j.cnki.kqyxyj.2016.03.022

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (3): 301-304.DOI: 10.13701/j.cnki.kqyxyj.2016.03.022

PEN载体传递ODN对人成骨样细胞细胞周期与成骨分化的影响

娄译心¹, 郑义², 申玉芹², 崔野¹, 孙晗¹, 孙新华^1*

1. 吉林大学口腔医院正畸科吉林长春 130021;
2. 吉林大学口腔医院牙周病科吉林长春 130021

收稿日期:2015-09-28 出版日期:2016-03-28 发布日期:2016-03-29
通讯作者: 孙新华,电话:0431-88796023
作者简介:娄译心(1989~ ),女,硕士在读,长春市人,主要从事口腔正畸诊治及相关机制的研究。

Effects of PEN-loading Oligodeoxynucleotide on the Cell Cycle and Osteogenic Differentiation of Osteoblastic Cells.

LOU Yi-xin¹, ZHENG Yi², SHEN Yu-qin², CUI Ye¹, SUN Han¹, SUN Xin-hua^1*

1. Department of Orthodontics, Hospital of Stomatology, Jilin University, Changchun 130021, China;
2. Department of Periodontology, Hospital of Stomatology, Jilin University, Changchun 130021, China

Received:2015-09-28 Online:2016-03-28 Published:2016-03-29

摘要/Abstract

摘要： 目的: 采用新型载体PEN对ODN进行传递,探讨MT01/PEN复合物对人成骨样细胞(MG63)细胞周期及成骨分化的影响。方法: 选取不同装载比例的MT01/PEN复合物,以MT01及S-MT01做对照,分别检测其对MG63细胞周期及成骨分化相关基因ALP活性、BMP2、OCN表达的影响。结果: MT01/PEN组Gl期、G2期细胞百分比降低,S期比例增高(P<0.01),碱性磷酸酶活性增加(P<0.01),OCN、BMP2蛋白表达增高(P<0.05)。结论: 以PEN为载体传递MT01能促进MG63的分化。

关键词: 人骨肉瘤成骨细胞, 寡核苷酸MT01, PEI衍生物, 分化

Abstract: Objective: To adopt a new vector PEN-loading ODN and evaluate its effects on the cell cycle and osteogenic differentiation of osteoblastic cells. Methods: Different ratios of MT01/PEN complex were transfected into MG63 cells, while MT01 and S-MT01 were negative controls. After that, the cell cycle, ALP activity, and the expression levels of BMP2 and OCN were detected. Results: In MT01/PEN group, cell percentages in Gl and G2 phase were decreased, while that in S phase was increased (P<0.01). Meanwhile, ALP activity was increased (P<0.01), and the expression of OCN and BMP2 were significantly up-regulated (P<0.05). Conclusion: The application of PEN as a carrier of ODN could promote the osteogenic differentiation of MG63 cells.

Key words: MG63 , ODN , MT01 , Derivative of , PEI, Differentiation

中图分类号:

R781.4

娄译心, 郑义, 申玉芹, 崔野, 孙晗, 孙新华. PEN载体传递ODN对人成骨样细胞细胞周期与成骨分化的影响[J]. 口腔医学研究, 2016, 32(3): 301-304.

LOU Yi-xin, ZHENG Yi, SHEN Yu-qin, CUI Ye, SUN Han, SUN Xin-hua. Effects of PEN-loading Oligodeoxynucleotide on the Cell Cycle and Osteogenic Differentiation of Osteoblastic Cells.[J]. Journal of Oral Science Research, 2016, 32(3): 301-304.

参考文献

[1] Hou X,Shen YQ,Zhang C,et al. A Specific Oligodeoxynucleotide Promotes the differentiation of Osteoblasts via ERK and p38 MAPK Pathways [J]. Int J Mol Sci,2012,13(7)∶7902-7914
[2] Zhu Y, Meng W, Hanagata N. Cytosine-phosphodiester-guanine oligodeoxynucleotide (CpG ODN)-capped hollow mesoporous silica particles for enzyme-triggered drug delivery [J]. Dalton Trans,2011,40(39)∶10203-10208
[3] Feng ZY,Shen YQ,Wang LY,et al. An oligodeoxynueleotide with Promising modulation activity for the proliferation and activation of osteoblast [J]. Ini J Mol Sci,2011,12(4)∶2543-2555
[4] 王艳波,新型PEI衍生材料的合成及在肿瘤基因递送中的应用研究[D].博士学位论文,吉林大学,2014年
[5] Yan X,Zhang Y,Zhang H,et al. Amphiphilic polyethylenimine (PEI) as highly efficient non-viral gene carrier [J]. Org Biomol Chem, 2014 ,12(12)∶1975-1982
[6] 王树平,郑义,申玉芹,等.PEN载ODN MT01复合物性能检测及其对成骨细胞增殖的影响[J].口腔医学研究,2015,6(012)∶1671-1651
[7] Zou W, Amcheslavsky A, Bar-Shavit Z.CpG oligodeoxynucleotides modulate the osteoclastogenic activity of osteoblasts via Toll-like receptor 9 [J]. Biol Chem,2003,278(19)∶16732-4
[8] Shen YQ,Feng ZY,Lin CT,et al. An Oligodeoxynucleotide That Induces Differentiation of Bone Marrow Mesenchymal Stem Cells to Osteoblasts in Vitro and Reduces Alveolar BoneLoss in Rats with Periodontitis [J]. Int J Mol Sci,2012,13(3)∶2877-92
[9] 申玉芹,孙新华,于丽,等.小寡核营酸(ODN)对大鼠骨髓间充质干细胞向成骨细胞分化的影响[J].吉林大学学报(医学版),2010,36(02)∶252-255
[10] 李东文,满大鹏,杜晓岩,等.特定序列寡核苷酸对人骨髓间充质干细胞增殖能力的影响[J].口腔医学研究2014,30(7)∶1671-7651
[11] 张晓敏,CpG ODN对人牙周膜细胞增殖分化的影响[D].硕士学位论文,吉林大学,2013年
[12] Ferreiro MG,Lloyd G,Bodmeier HR. Alginate/Poly-L-Lysine Microparticles for the Intestinal Delivery of Antisense Oligonucleotides [J]. Pharm Res, 2002, 19(6)∶755-64
[13] González Ferreiro M1, Tillman LG, Hardee G,et al. The effect of circulation lifetime and drug-to-lipid ratio of intravenously administered lipid nanoparticles on the biodistribution and immunostimulatory activity of encapsulated CpG-ODN [J]. J Drug Target,2008,16(7-8)∶564-577
[14] Rosen V. BMP2 signaling in bone development and repair [J]. Cytokine Growth Factor Rev, 2009, 20(5-6)∶475-480
[15] Feng ZY,Chen QL,ZhangY,et al. Outcome of Postsurgical Sequential Funetional Exercise of Jaw Fracture [J]. J Craniofac Surg,2009,20(1)∶46-8

PEN载体传递ODN对人成骨样细胞细胞周期与成骨分化的影响

Effects of PEN-loading Oligodeoxynucleotide on the Cell Cycle and Osteogenic Differentiation of Osteoblastic Cells.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	张师贤, 林成泉, 谢长富, 陈伟辉. Gli1抑制剂GANT61对小鼠牙胚体外发育的影响[J]. 口腔医学研究, 2021, 37(1): 23-27.
[2]	张皓, 杨国斌. 微小RNA-1895参与小鼠牙乳头细胞成牙本质向分化的研究[J]. 口腔医学研究, 2021, 37(1): 28-32.
[3]	周何洁, 李胜鸿, 彭培钊, 唐榕, 李艾莲, 曾锦. “红色复合体”致病菌裂解液对人牙周膜细胞成骨分化能力的影响[J]. 口腔医学研究, 2020, 36(9): 839-843.
[4]	关孟莹, 何丽娜, 潘爽, 李艳萍, 刘会梅, 牛玉梅. 经典Wnt信号通路对hDPSCs增殖、迁移和成牙本质向分化的影响[J]. 口腔医学研究, 2020, 36(9): 861-865.
[5]	邱吟枫, 汤颖, 沈忆芬, 刘超, 沈昊, 顾永春, 于金华. 氟暴露对人牙周膜干细胞增殖及成骨分化的影响[J]. 口腔医学研究, 2020, 36(9): 866-870.
[6]	马兰, 禹怡君, 刘含笑, 刘超, 苗雷英. 黄连素促人牙髓干细胞成骨/成牙本质细胞向分化[J]. 口腔医学研究, 2020, 36(7): 639-643.
[7]	何林, 何淞, 吴稀, 杨森. 人根尖乳头干细胞在羟基磷灰石和聚多巴胺-羟基磷灰石上的粘附、增殖及成骨分化[J]. 口腔医学研究, 2020, 36(6): 523-527.
[8]	邬扬绚, 向黎, 黄志强, 褚金海. 釉基质蛋白衍生物调节牙龈卟啉单胞菌感染牙周膜干细胞成骨分化的作用及机制[J]. 口腔医学研究, 2020, 36(6): 534-538.
[9]	霍冬梅, 徐燕, 胡韶光, 汪芹芹, 王荣海. 人血小板裂解液对牙髓间充质细胞成骨分化的影响[J]. 口腔医学研究, 2020, 36(6): 539-543.
[10]	陈振宇, 潘颖菁, 张艳, 贺玺, 黄达鸿. 葛根素对高浓度糖皮质激素环境下MC3T3-E1前成骨细胞生物学性能的影响[J]. 口腔医学研究, 2020, 36(6): 544-547.
[11]	王楠, 周延民. 石墨烯材料在口腔医学领域的应用及生物安全性研究[J]. 口腔医学研究, 2020, 36(5): 410-412.
[12]	何梦婷, 白丁. 牙囊在牙萌出的作用及其调控机制研究进展[J]. 口腔医学研究, 2020, 36(2): 107-110.
[13]	王素文, 赖思煜, 习利军, 王利宏. 黄芪甲苷调节人牙周膜细胞炎症反应及成骨分化的作用及机制研究[J]. 口腔医学研究, 2020, 36(2): 121-125.
[14]	殷晓薇, 张爽, 邓皓天, 何丽娜, 李艳萍, 张巍巍, 潘爽, 牛玉梅. 整合素α6对hDPSCs增殖和成牙本质向分化的影响[J]. 口腔医学研究, 2020, 36(12): 1132-1136.
[15]	卢永超, 王伟, 吕翱, 刘逸卿, 杜毅. 炎症微环境下骨髓间充质干细胞骨向分化中Periostin基因表达研究[J]. 口腔医学研究, 2020, 36(12): 1148-1152.