口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (12): 1306-1309.DOI: 10.13701/j.cnki.kqyxyj.2017.12.017

• 临床研究论著 • 上一篇    下一篇

种植体周围黏膜炎在药物干预前后龈沟液的代谢组学分析

张雨诗1,2,邵艳琳3,芦帅2,钟科1,2*   

  1. 1. 西南医科大学口腔医学院 四川 泸州 646000;
    2. 成都军区机关医院 四川 成都 610041;
    3. 南充市中心医院口腔科 四川 南充 637000
  • 收稿日期:2017-07-24 出版日期:2017-12-20 发布日期:2018-01-03
  • 通讯作者: 钟科,E-mail:37435387@qq.com
  • 作者简介:张雨诗(1991~ ),女,硕士在读, 医师,主要从事口腔种植修复学研究工作。
  • 基金资助:
    成都军区机关医院院管课题(编号:YG201405)

Metabonomics Study on Peri-implant Mucositis before and after Drug Intervention.

ZHANG Yu-shi1,2, SHAO Yan-lin3, LU Shuai2, ZHONG Ke1,2*.   

  1. 1.School of Stomatology, Southwest Medical University, Luzhou 646000, China;
    2. Authority Hospital of Chengdu Military Region, Chengdu 610041, China;
    3. Nanchong Central Hospital of Stomatology, Nanchong 637000,China.
  • Received:2017-07-24 Online:2017-12-20 Published:2018-01-03

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摘要： 目的: 探讨种植体周围黏膜炎在药物(盐酸米诺环素)干预前后龈沟液(GCF)的代谢组学特征及代谢产物差异,寻找种植体周围黏膜炎可能的生物标记物。方法: 30例接受种植牙患者,健康种植体组(A组)、种植体周围黏膜炎组(B组)、药物干预后组(C组)GCF,A组15例,B组与C组15例,每组随机选取5例样本,分别采集GCF应用超髙效液相色谱-四级杆飞行时间质谱联用(UHPLC/Q-TOF-MS)仪进行检测分析后,在线数据库Metlin library、人类代谢物数据库(HMDB)进行精确的比对检索。结果: A与B组、B与C组、A与C组间分别有10个、5个、4个差异性代谢产物有统计学意义(P<0.05)。结论: 黄嘌呤、溶血磷脂酰胆碱、溶血磷脂酰乙醇胺可能是种植体周围黏膜炎潜在的标志物。

关键词: 种植体周围黏膜炎, 超高效液相色谱, 四级杆飞行时间质谱, 代谢组学

Abstract: Objective: To discuss the gingival sulcus fluid (GCF) metabolomics characteristics and metabolic product difference in peri-implant mucositis before and after drug (minocycline hydrochloride) intervention, and to find the implant mucositis potential biomarkers. Methods: Thirty patients receiving dental implant were recruited and divided into group A (health implant), group B (peri-implant mucositis), and group C (drug intervention), with 15 cases in group A, 15 cases in group B and group C. Five cases were randomly selected from each group. GCF was collected and analyzed with UHPLC/Q-TOF-MS instrument. The results were compared accurately with online database Metlin Library and human metabolites database (HMDB). Results: There were 10, 5, and 4 different metabolites between A and B, B and C, and A and C (P<0.05). Conclusion: Hemolytic phosphatidyl choline and haemolytic phosphatidyl ethanolamine might be potential marker for the peri-implant mucositis.

Key words: Peri-implant mucositis , Ultra high performance liquid chromatography , Quadrupole-time-of-flight-mass spectrometry, Metabonomics

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