镁离子对人牙周韧带细胞体外成骨能力的影响

doi:10.13701/j.cnki.kqyxyj.2017.06.008

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (6): 609-613.DOI: 10.13701/j.cnki.kqyxyj.2017.06.008

镁离子对人牙周韧带细胞体外成骨能力的影响

姜雨汐^1,2,3, 张佳^1,2#, 魏凌飞², 曲伟栋², 刘玲玲¹, 石方玉¹, 江久汇^3*, 李翠英^3*, 刘树泰^2*

1. 滨州医学院山东烟台 264000;
2. 烟台市口腔医院山东烟台 264000;
3. 北京大学口腔医院北京 100081

收稿日期:2016-11-29 出版日期:2017-06-20 发布日期:2017-06-26
通讯作者: 刘树泰,E-mail:dentistliu@163.com李翠英,E-mail:licuiying_67@163.com江久汇,E-mail:drjiangw@163.com#为共同第一作者
作者简介:姜雨汐(1988~ ),女,山东人,硕士,医师,主要从事牙周病学研究。张佳(1990~ ),女,山东人,硕士,医师,主要从事种植学研究。
基金资助:
山东省“泰山学者海外特聘专家”建设平台项目(编号:TSHW20120233)
山东省自然科学基金(编号:ZR2014HP019)
山东省医药卫生发展计划(编号:2011QW033)

Effect of Magnesium Ion on Human Periodontal Ligament Cells Osteogenic Capability in Vitro.

JIANG Yu-xi^1,2,3, ZHANG Jia^1,2#, WEI Ling-fei², QU Wei-dong², LIU Ling-ling¹, SHI Fang-yu¹, JIANG Jiu-hui^3*,LI Cui-ying^3*, LIU Shu-tai^2*.

1. Binzhou Medical University, Yantai 264000, China;
2. Stomatological Hospital of Yantai, Yantai 264000, China;
3. Peking University School and Hospital of Stomatology, Beijing 100081, China.

Received:2016-11-29 Online:2017-06-20 Published:2017-06-26

摘要/Abstract

摘要： 目的:探究不同浓度Mg²⁺对hPDLCs成骨向分化的影响,为后续牙周组织再生实验选择合适的Mg²⁺注入浓度提供依据。方法:取P3-P5代hPDLCs于Mg²⁺浓度分别为0、10、15、25、35、50 mmol/L条件中常规培养,用CCK-8法检测hPDLCs增殖情况。成骨诱导后比较各组ALP活性差异及茜素红矿化结节着色情况,进行RT-qPCR检测成骨相关基因Runx2、ALP、Col1、OPN及Bglap的表达差异。采用SPSS16.0软件,运用单因素方差分析进行统计学分析。结果:10、15、25 mmol/L Mg²⁺浓度可促进细胞增殖并提高ALP活性,35、50 mmol/L Mg²⁺浓度抑制细胞增殖及ALP活性。结论:适宜浓度镁离子(0~25 mmol/L)可促进hPDLCs早期成骨分化并抑制矿化。

关键词: 牙周韧带细胞, 镁离子, 细胞增殖, 成骨分化, 骨桥蛋白

Abstract: Objective: To explore the effect of Mg²⁺ on hPDLCs osteogenic differentiation capability, and to determine the appropriate concentration range of Mg²⁺ to provide the basis for choosing Mg²⁺ infuse concentration in future periodontal regeneration research. Methods: hPDLCs stimulated with the presence of Mg²⁺ at various concentrations (from 0 to 50 mmol/L) were cultured in control medium and osteogenic medium, respectively. CCK8 was used to estimate the cell proliferation level, ALP activity assays were performed to detect the ALP activity differences in each group and alizarin red staining was used to determine the calcium mineral density. The mRNA expression levels of Runx2, ALP, Col1, OPN, and Bglap in hPDLCs with Mg²⁺ at concentrations of 0 and 15 mmol/L were examined by RT-qPCR of post-osteogenic induction. The results were analyzed using analysis of variance (ANOVA) and Student-Newman-Keuls (SNK) test for pairwise comparisons implemented in the SPSS 16.0 software. Results: Mg²⁺ at concentrations of 10, 15, and 25 mmol/L promoted hPDLCs proliferation and ALP activity, whereas Mg²⁺ at concentrations of 35 and 50 mmol/L remarkably inhibit hPDLCs proliferation and ALP activity. Conclusion: Proper Mg²⁺ concentration (0-25mmol/L) can promote early-term osteogenic differentiation of hPDLCs and inhibit mineralization.

Key words: Periodontal ligament cells, Magnesium ion , Cell proliferation , Osteogenic differentiation, Osteopontin

中图分类号:

R781.4

姜雨汐, 张佳, 魏凌飞, 曲伟栋, 刘玲玲, 石方玉, 江久汇, 李翠英, 刘树泰. 镁离子对人牙周韧带细胞体外成骨能力的影响[J]. 口腔医学研究, 2017, 33(6): 609-613.

JIANG Yu-xi, ZHANG Jia, WEI Ling-fei, QU Wei-dong, LIU Ling-ling, SHI Fang-yu, JIANG Jiu-hui,LI Cui-ying, LIU Shu-tai.. Effect of Magnesium Ion on Human Periodontal Ligament Cells Osteogenic Capability in Vitro.[J]. Journal of Oral Science Research, 2017, 33(6): 609-613.

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镁离子对人牙周韧带细胞体外成骨能力的影响

Effect of Magnesium Ion on Human Periodontal Ligament Cells Osteogenic Capability in Vitro.

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