口腔医学研究 ›› 2019, Vol. 35 ›› Issue (1): 94-97.DOI: 10.13701/j.cnki.kqyxyj.2019.01.022

• 口腔生物学研究 • 上一篇    下一篇

SRSF3调控CTLA-4外显子3的可变剪接的研究

黄珺, 郭继华, 樊明文*   

  1. 武汉大学口腔医学院,湖北省口腔基础医学重点实验室-省部共建国家重点实验室培育基地, 口腔生物医学教育部重点实验室 湖北 武汉 430079
  • 收稿日期:2018-07-27 出版日期:2019-01-18 发布日期:2019-01-28
  • 通讯作者: 樊明文,E-mail:fmwmail@whu.edu.cn
  • 作者简介:黄珺(1992~ ),女,湖北武汉人,硕士,主要从事牙体牙髓龋病学相关研究和临床工作。
  • 基金资助:
    国家自然科学基金(编号:81570972)

Alternative Splicing of CTLA-4 Exon3 is Regulated by SRSF3

HUANG Jun, GUO Ji-hua, FAN Ming-wen*   

  1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China
  • Received:2018-07-27 Online:2019-01-18 Published:2019-01-28

摘要: 目的:研究在肿瘤中高表达的可变剪接因子SRSF3对肿瘤免疫治疗相关因子CTLA-4外显子3的可变剪接的调控作用。方法:在人胚胎肾上皮细胞293细胞中分别共转染CTLA-4模拟基因和SRSF3特异性siRNA,CTLA-4模拟基因和SRSF3过表达质粒来敲低和过表达SRSF3,然后用逆转录PCR检测SRSF3敲低和过表达后对CTLA-4的两种可变剪接亚型,跨膜型CTLA-4(flCTLA-4)和可溶性CTLA-4(sCTLA-4)表达的影响。结果:在293细胞中抑制SRSF3的表达时,flCTLA-4可变剪接亚型的表达增加,sCTLA-4可变剪接亚型的表达降低,在293细胞中过表达SRSF3时,得到了相反的结果。结论:SRSF3作为肿瘤中高表达的原癌基因参与调控CTLA-4外显子3的可变剪接,并使flCTLA-4可变剪接亚型的表达降低,sCTLA-4可变剪接亚型的表达增加。

关键词: SRSF3, CTLA-4, 可变剪接

Abstract: Objective: To investigate the effects of SRSF3 on alternative splicing of CTLA-4 exon3. Methods: CTLA-4 minigene was co-transfected with SRSF3 siRNA and SRSF3 plasmid in human embryonic renal epithelial cells (293 cell) respectively to knock down and overexpress the expression of SRSF3. The effects on CTLA-4 splicing isoforms flCTLA-4 and sCTLA-4 were analyzed by RT-PCR. Results: Knock down of SRSF3 increased the expression of flCTLA-4 and inhibited sCTLA-4 product in 293 cells. Overexpression of SRSF3 showed the contrary result. Conclusion: SRSF3 regulates the alternative splicing of CTLA-4 exon 3, down-regulates the expression of flCTLA-4, and increases the expression of sCTLA-4 in human embryonic renal epithelial cells.

Key words: SRSF3, CTLA-4, Alternative splicing