咀嚼对应激大鼠杏仁核和前额叶皮质中GAT-1的影响

doi:10.13701/j.cnki.kqyxyj.2020.08.008

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (8): 736-740.DOI: 10.13701/j.cnki.kqyxyj.2020.08.008

咀嚼对应激大鼠杏仁核和前额叶皮质中GAT-1的影响

杨佳迪¹, 边策², 王旭¹, 李荷香^1*

1. 河北医科大学口腔医学院·口腔医院基础教研室,河北省口腔医学重点实验室河北石家庄 050017;
2. 首都医科大学附属北京口腔医院正畸科北京 100050

收稿日期:2019-11-25 出版日期:2020-08-28 发布日期:2020-08-18
通讯作者: *李荷香,E- mail:lihexiang-2008@163.com
作者简介:杨佳迪(1994~ ),女,河北邢台人,医师,硕士在读,研究方向:磨牙症及全口义齿修复。

Effects of Chewing on GAT-1 in Amygdala and Frontal Cortex of Stressed Rats

YANG Jiadi¹, BIAN Ce², WANG Xu¹, LI Hexiang^1*

1. Department of Basic Teaching and Research, School of Hospital of Stomatology, Hebei Medical University & Hebei Key laboratory of Stomatology, Shijiazhuang 050017, China;
2. Department of Orthodontics, Beijing Hospital of Stomatology, Capital Medical University, Beijing 100050, China

Received:2019-11-25 Online:2020-08-28 Published:2020-08-18

摘要/Abstract

摘要： 目的: 探讨咀嚼对应激大鼠杏仁核、前额叶皮质GABA再摄取的影响。方法: 将成年雄性SD大鼠52只分成对照组(CO组)、应激组(ST组)、应激+咀嚼组(SC组)。除CO组外,各组大鼠均置于固定筒中束缚30 min。造模同时,SC组给予直径5 mm木棍以供其咀嚼,咀嚼少于15 min者被丢弃。通过旷场实验记录各组大鼠运动得分以测试其焦虑程度,实验结束后随机挑选大鼠18只(每组6只)立即处死,取杏仁核、前额叶皮质组织检测γ-氨基丁酸转运体-1(GAT-1)mRNA表达情况。结果: 与CO组相比,ST组大鼠OF值显著降低,杏仁核与前额叶皮质GAT-1 mRNA表达显著升高(P<0.05);SC组大鼠OF值较ST组显著升高,杏仁核与前额叶皮质GAT-1 mRNA表达显著降低(P<0.05),但与CO组比较差异无统计学意义(P>0.05)。结论: 咀嚼可以通过纠正杏仁核、前额叶皮质GAT-1的异常表达调节GABA代谢失常而发挥缓解压力的作用。

关键词: 咀嚼, γ-氨基丁酸, γ-氨基丁酸转运体, 应激衰减效应

Abstract: Objective: To investigate the effect of mastication on GABA reuptake in amygdala and frontal cortex of rats. Methods: 52 SD rats were divided into control group (CO group), stress group (ST group), and stress + chewing group (SC group). Except for CO group, rats in each group were placed in a fixed tube for 30 minutes. Meanwhile, the SC group was given a 5mm diameter stick for chewing, and those chewing for less than 15 minutes were discarded. The motor scores of each group were recorded in Open-Field test. At the end of the experiment, 18 rats (6 in each group) were randomly selected and killed immediately. Results: Compared with CO group, the OF value of ST group was significantly lower, and the expression of GAT-1 mRNA in amygdala and frontal cortex was significantly higher (P<0.05). The OF value of SC group was significantly higher than ST group, and the expression of GAT-1 mRNA in amygdala and frontal cortex was significantly lower (P<0.05). There was no significant difference between SC group and CO group (P>0.05). Conclusion: Mastication can relieve the stress by correcting the abnormal expression of GAT-1 in amygdala and frontal cortex.

Key words: mastication, γ-aminobutyric acid, γ-aminobutyric acid transporter-1, stress-attenuation effects

杨佳迪, 边策, 王旭, 李荷香. 咀嚼对应激大鼠杏仁核和前额叶皮质中GAT-1的影响[J]. 口腔医学研究, 2020, 36(8): 736-740.

YANG Jiadi, BIAN Ce, WANG Xu, LI Hexiang. Effects of Chewing on GAT-1 in Amygdala and Frontal Cortex of Stressed Rats[J]. Journal of Oral Science Research, 2020, 36(8): 736-740.

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咀嚼对应激大鼠杏仁核和前额叶皮质中GAT-1的影响

Effects of Chewing on GAT-1 in Amygdala and Frontal Cortex of Stressed Rats

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