微小RNA-1895参与小鼠牙乳头细胞成牙本质向分化的研究

doi:10.13701/j.cnki.kqyxyj.2021.01.007

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (1): 28-32.DOI: 10.13701/j.cnki.kqyxyj.2021.01.007

微小RNA-1895参与小鼠牙乳头细胞成牙本质向分化的研究

张皓, 杨国斌^*

武汉大学口腔医学院,湖北省口腔基础医学重点实验室-省部共建国家重点实验室培育基地,口腔生物医学教育部重点实验室(武汉大学) 湖北武汉 430079

收稿日期:2020-04-22 出版日期:2021-01-28 发布日期:2021-01-22
通讯作者: *杨国斌,E-mail:guobin.yang@whu.edu.cn
作者简介:张皓(1994~ ),女,河北承德人,硕士,研究方向:牙发育。
基金资助:
国家自然科学基金(编号:81570942)

Regulation of Odontoblastic Differentiation by miR-1895 in Mouse Dental Papilla Cells

ZHANG Hao, YANG Guobin^*

Hubei-MOST KLOS & KLOBME, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China

Received:2020-04-22 Online:2021-01-28 Published:2021-01-22

摘要/Abstract

摘要： 目的: 研究微小RNA(miRNA,miR)-1895在小鼠牙乳头细胞向成牙本质细胞向分化过程中的功能。方法: 建立小鼠牙乳头细胞向成牙本质细胞向分化的模型,检测miR-1895在此过程中的表达。使用生物信息学分析miR-1895在Klf4 mRNA中的结合位点。向细胞内转染miR-1895 inhibitor,利用Western blot和Real-time PCR检测抑制miR-1895后,对小鼠牙乳头细胞向成牙本质细胞向分化的影响。结果: 在小鼠牙乳头细胞向成牙本质细胞向分化过程中miR-1895表达降低。Klf4 mRNA中存在miR-1895 结合位点。抑制细胞内miR-1895表达后,细胞内成牙本质细胞相关基因表达增加。结论: 在小鼠牙乳头细胞向成牙本质细胞向分化的过程中,miR-1895抑制细胞分化,可能参与分化调节过程。

关键词: 牙乳头细胞, 成牙本质细胞向分化, 微小RNA-1895

Abstract: Objective: To investigate the function of miR-1895 during the odontoblastic differentiation of mouse dental papilla cells. Methods: The model of odontoblastic differentiation of mouse dental papilla cells was established, and the expression of miR-1895 was detected during this process. The binding site of miR-1895 in Klf4 mRNA was analyzed by using bioinformatics. Western blot and real-time PCR were employed to detect the influence of miR-1895 on odontoblastic differentiation of mouse dental papilla cells after transfection with miRNA inhibitor. Results: The expression of miR-1895 decreased during the odontoblastic differentiation of mouse dental papilla cells. There was a binding site to miR-1895 in Klf4 mRNA. After inhibiting miR-1895, the expression of related genes of odontoblastic differentiation increased. Conclusion: miR-1895 inhibited the odontoblastic differentiation of mouse dental papilla cells and might participate in regulating this process.

Key words: dental papilla cells, odontoblastic differentiation, miR-1895

张皓, 杨国斌. 微小RNA-1895参与小鼠牙乳头细胞成牙本质向分化的研究[J]. 口腔医学研究, 2021, 37(1): 28-32.

ZHANG Hao, YANG Guobin. Regulation of Odontoblastic Differentiation by miR-1895 in Mouse Dental Papilla Cells[J]. Journal of Oral Science Research, 2021, 37(1): 28-32.

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微小RNA-1895参与小鼠牙乳头细胞成牙本质向分化的研究

Regulation of Odontoblastic Differentiation by miR-1895 in Mouse Dental Papilla Cells

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