口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (9): 943-945.DOI: 10.13701/j.cnki.kqyxyj.2017.09.008

• 基础研究论著 • 上一篇    下一篇

3种植物提取物联用对变形链球菌致龋力的影响

董伟1,2，陈锐1,2，颜权1,2，包广洁2*   

  1. 1. 兰州大学口腔医学研究所 甘肃 兰州 730000；
    2. 西北民族大学口腔医学国家民委重点实验室 甘肃 兰州 730000
  • 收稿日期:2017-03-22 出版日期:2017-09-20 发布日期:2017-09-27
  • 通讯作者: 包广洁,Email:2645784430@qq.com
  • 作者简介:董伟(1996~ ),男,甘肃庆阳人,学士,主要从事口腔微生物研究。
  • 基金资助:
    兰州大学学生创新创业行动计划项目(编号:20161073001556)

Effects of Three Plant Extracts on Cariogenic Tolerance of Streptococcus Mutans

DONG Wei1,2, CHEN Rui1,2, YAN Quan1,2, BAO Guang-jie2*   

  1. 1. School of Stomatology, Lanzhou University, Lanzhou 730000, China;
    2. Key Lab of Stomatology of State Ethnic Affairs Commission, Northwest University for Nationalities, Lanzhou 730000, China.
  • Received:2017-03-22 Online:2017-09-20 Published:2017-09-27

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摘要： 目的:探究绿茶、野菊花、薄荷提取物及其混合物对变形链球菌增殖、产酸及粘附能力的影响。方法:变形链球菌在含有不同浓度植物提取物的MS培养基中培养24 h后,进行离心,清洗,将菌细胞混悬于一定体积蒸馏水中,分光光度计测出A值。使用pH仪测定离心后上清液pH值。玻片粘附法观察提取物对变形链球菌粘附能力的影响。结果:各浓度的提取物及其混合物对变形链球菌增殖、产酸、粘附均有抑制作用(P＜0.05)。浓度为24 g/L时,绿茶较其他提取物抑制变形链球菌增殖能力强(P＜0.01)。浓度为0.75 g/L、1.5 g/L、3 g/L时,混合物抑制变形链球菌粘附能力较同浓度其他提取物强(P＜0.05)。结论:3种植物提取物均具有一定防龋能力,在抑制变形链球菌粘附能力方面三者可协同发挥作用。

关键词: 绿茶, 野菊花, 薄荷, 变形链球菌, 致龋力

Abstract: Objective: To study the effects of green tea, wild chrysanthemum, mint extract and their compound on the proliferation, acid production and adhesion of Streptococcus mutans. Methods: Streptococcus mutans were cultured in MS medium containing different concentrations of plant extracts for 24 h, then centrifuged and washed. The bacteria cells were suspended in a certain volume of distilled water, and the optical density was measured by spectrophotometer. The pH of the supernatant was measured using a pH meter after centrifugation. The effect of the extract on the adhesion of Streptococcus mutans was observed by slide adhesion method. Results: Each extract and its compound inhibited the proliferation, acid production and adhesion of Streptococcus mutans (P<0.05). When the concentration was 24 g/L, the effect of green tea on the proliferation of Streptococcus mutans was stronger than that of other extracts (P<0.01). When the concentration was 0.75 g /L, 1.5 g / L and 3 g / L, the inhibitory effect of the compoundon Streptococcus mutans adhesion was stronger than that of other extracts (P<0.05) . Conclusion: All the three plant extracts have a certain anti-caries ability, and showed significant synergistic antibacterial activity.

Key words: Green tea, Wild chrysanthemum, Mint , Streptococcus mutans , Cariogenicity

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