口腔医学研究

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (9): 875-878.DOI: 10.13701/j.cnki.kqyxyj.2020.09.017

• 口腔抗菌材料研究 • 上一篇    下一篇

Farnesol纳米胶束的构建及对变形链球菌的抑菌作用研究

赵霞1,2, 徐嘉昊3, 夏商2, 张晨2, 王万春4*   

  1. 1.青岛大学口腔医学院 山东 青岛 266003;
    2.青岛市口腔医院儿童口腔科 山东 青岛 266001;
    3.中国海洋大学药学院 山东 青岛 266100;
    4.青岛市口腔医院口腔黏膜科 山东 青岛 266001
  • 收稿日期:2020-02-14 出版日期:2020-09-28 发布日期:2020-09-15
  • 通讯作者: *王万春,E-mail: kqwwch@126.com
  • 作者简介:赵霞(1976~),女,山东青岛人,硕士在读,副主任医师,主要从事儿童口腔医学的临床研究工作。
  • 基金资助:
    青岛市2019年度医药科研指导计划(编号:2019-WJZD138);青岛市医疗卫生重点学科建设项目

Construction of Water-soluble Farnesol Nano-micelle and Its Antibacterial Effect on Streptococcus Mutans

ZHAO Xia1,2, XU Jiahao3, XIA Shang2, ZHANG Chen2, WANG Wanchun4*   

  1. 1. School of Stomatology, Qingdao University, Qingdao 266003, China;
    2. Department of Pediatric Stomatology, Qingdao Stomatological Hospital, Qingdao 266001, China;
    3. School of Pharmacy, Ocean University of China, Qingdao 266100, China;
    4. Department of Oral Mucosa, Qingdao Stomatological Hospital, Qingdao 266001, China
  • Received:2020-02-14 Online:2020-09-28 Published:2020-09-15

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摘要： 目的: 构建水溶性Farnesol纳米胶束,并研究其对变形链球菌的抑菌作用。方法: 采用乳化溶剂扩散法制备Farnesol纳米胶束。通过Malvern粒度仪检测粒径、聚合物分散性系数(PDI)、Zeta电位3项指标,计算载药量及包封率;并考察其稳定性、水溶性、体外释放行为等。通过扫描电镜评价Farnesol纳米胶束对变形链球菌菌斑生物膜形态的影响。采用微板法检测菌悬液中钙离子浓度,探讨Farnesol纳米胶束对变形链球菌产酸及釉质脱矿的抑制作用。结果: Farnesol纳米胶束水溶液澄清,无沉淀。粒径为(19.78±0.21) nm,Zeta电势为(-12.2±0.4) mV,PDI为0.089±0.040,载药量为(8.3±0.5)%,包封率为(46.89±1.10)%,12 h累计释放70%。该纳米胶束能够抑制变形链球菌抑制菌斑生物膜的形成,降低产酸能力抑制釉质脱矿,且呈浓度依赖性。结论: Farnesol纳米胶束制备良好,具有水溶性、稳定性和缓释性等特点,并可抑制变形链球菌的生长,降低其产酸能力,对龋病的防治有重要的临床指导价值。

关键词: 法尼醇, 纳米胶束, 变形链球菌, 菌斑生物膜, 细菌产酸

Abstract: Objective: To construct water-soluble Farnesol nano-micelle and study its antibacterial effect on streptococcus mutans. Methods: Farnesol micelles were prepared by emulsion solvent diffusion method. Particle size, polymer dispersion coefficient (PDI), and zeta potential were measured by Marvin laser particle size analyzer. It’s stability, water solubility, and in vitro release behavior were investigated. The effect of Farnesol nano-micelles on the morphology of streptococcus mutans plaque biofilm was evaluated by SEM. The calcium ion concentration in the bacterial suspension was detected by microplate method, and the inhibitory effect of Farnesol nano-micelles on acid production and enamel demineralization of streptococcus mutans was investigated. Results: Farnesol nano-micelle aqueous solution was clarified without precipitation. The particle size was (19.78±0.21) nm, the zeta potential was (-12.2±0.4) mV, the PDI was (0.089±0.040), the drug loading was (8.3±0.5) %, the encapsulation rate was (46.89±1.10) %, and the cumulative release was 70% at 12h. The nanometer micelle could inhibit the formation of plaque biofilm by streptococcus mutans, reduce the acid-producing capacity, and inhibit the demineralization of enamel in a concentration-dependent way. Conclusion: Farnesol micelles are well prepared and have the characteristics of water solubility, stability, and slow release. It can inhibit the growth of streptococcus mutans and reduce its acid producing capacity, which has important clinical value for the prevention and treatment of caries.

Key words: Farnesol, micelle, streptococcus mutans, plaque biofilm, bacteria produce acid