MicroRNA 34a-5p对成骨细胞炎症反应及分化的影响

doi:10.13701/j.cnki.kqyxyj.2017.11.002

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (11): 1139-1142.DOI: 10.13701/j.cnki.kqyxyj.2017.11.002

MicroRNA 34a-5p对成骨细胞炎症反应及分化的影响

王贵玲, 杨谛, 郭佳杰, 于雅琼, 仇丽鸿^*

中国医科大学口腔医学院牙体牙髓病科,辽宁省口腔疾病重点实验室辽宁沈阳 110002

收稿日期:2017-05-24 出版日期:2017-11-20 发布日期:2017-11-29
通讯作者: 仇丽鸿,E-mail:drqlh@yahoo.com
作者简介:王贵玲(1988~ ),女,河南濮阳人,硕士,医师,主要从事牙体牙髓病学研究。
基金资助:
国家自然科学基金(编号:81500843)
沈阳市科学技术项目计划(编号:F15-199-1-56)

Effects of MicroRNA 34a-5p on Inflammation and Differentiation of Osteoblast

WANG Gui-ling, YANG Di, GUO Jia-jie, YU Ya-qiong, QIU Li-hong^*

Liaoning Key Laboratory of Oral Diseases, Department of Endodontics, School of Stomatology, China Medical University, Shenyang 110002, China.

Received:2017-05-24 Online:2017-11-20 Published:2017-11-29

摘要/Abstract

摘要： 目的:探究microRNA 34a-5p(miR-34a-5p)对成骨细胞炎症反应及分化的调节作用,并初步探究其相关机制。方法:脂质体法瞬时转染人成骨样细胞系MG63使细胞中miR-34a-5p表达增强,同时设通用阴性对照(NC)组。牙髓卟啉单胞菌(Porphyromonas endodontalis,P.e)来源的不同浓度脂多糖(lipopolysaccharide,LPS)作用于MG63后,检测细胞内miR-34a-5p表达; 取20 mg/L P.e-LPS作用24 h后,检测细胞内IL-6表达。成骨诱导48 h后,检测细胞中成骨分化标志物以及Notch1受体的表达。结果:P.e-LPS作用于MG63细胞后,miR-34a-5p表达被抑制(P<0.05)。 MiR-34a-5p转染细胞后,与NC组相比,细胞内IL-6表达减少(P<0.01);20 mg/L P.e-LPS作用下,miR-34a-5p组IL-6表达较NC组下降更为明显(P<0.01)。成骨细胞诱导分化48 h后,miR-34a-5p组中分化标志物表达较NC对照组均显著增加(P<0.05),而Notch1受体表达被抑制(P<0.05)。结论:MiR-34a-5p可能通过Notch1信号通路,发挥抑制成骨细胞炎症和促进成骨分化的作用。

关键词: MicroRNA, 34a-5p, 成骨细胞, 炎症, 分化

Abstract: Objective: To investigate the effect of microRNA 34a-5p (miR-34a-5p) on the inflammation and differentiation of osteoblasts. Methods: MG63 was transfected with miR-34a-5p mimics to ensure a higher expression than general negative control (NC). Expression of miR-34a-5p was detected after MG63 was stimulated by different concentrations of lipopolysaccharide (LPS) from porphyromonas endodontalis (P.e). Transfected cells were stimulated by 20 mg/L P.e-LPS for 24 h, and expression of IL-6 was detected. Expressions of osteogenic differentiation markers and Notch1 were detected after osteogenic induction for 48 h. Results: Expression of miR-34a-5p in MG63 was down-regulated by P.e-LPS with different concentrations(P<0.05). MiR-34a-5p inhibited the expression of IL-6 significantly both with and without the stimulation of P.e-LPS(P<0.01). Osteogenic makers in MG63 were promoted by miR-34a-5p(P <0.05)and Notch1 was down-regulated(P<0.05). Conclusion: MiR-34a-5p inhibits the inflammation and promotes the differentiation of osteoblast, and Notch1 may serve as a target during the process.

Key words: MicroRNA 34a-5p, Osteoblast, Inflammation, Cell differentiation

中图分类号:

R329.2

王贵玲, 杨谛, 郭佳杰, 于雅琼, 仇丽鸿. MicroRNA 34a-5p对成骨细胞炎症反应及分化的影响[J]. 口腔医学研究, 2017, 33(11): 1139-1142.

WANG Gui-ling, YANG Di, GUO Jia-jie, YU Ya-qiong, QIU Li-hong. Effects of MicroRNA 34a-5p on Inflammation and Differentiation of Osteoblast[J]. Journal of Oral Science Research, 2017, 33(11): 1139-1142.

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MicroRNA 34a-5p对成骨细胞炎症反应及分化的影响

Effects of MicroRNA 34a-5p on Inflammation and Differentiation of Osteoblast

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