N-乙酰半胱氨酸对脂多糖诱导的血管内皮细胞炎症损伤的影响

doi:10.13701/j.cnki.kqyxyj.2020.04.017

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (4): 377-381.DOI: 10.13701/j.cnki.kqyxyj.2020.04.017

• 牙周病学与黏膜病学研究 • 上一篇下一篇

N-乙酰半胱氨酸对脂多糖诱导的血管内皮细胞炎症损伤的影响

张贞祯, 熊婷, 郑睿, 黄佳琳, 郭玲^*

西南医科大学附属口腔医院修复科四川泸州 646000

收稿日期:2019-07-01 出版日期:2020-05-28 发布日期:2020-05-28
通讯作者: 郭玲,E-mail:2005202@foxmail.com
作者简介:张贞祯(1994~ ),女,四川广安人,硕士在读,主要从事口腔种植修复的研究工作。
基金资助:
四川省科技厅面上项目(编号:2017JY0111); 西南医科大学重点项目(编号:2016013)

Effect of N-acetylcysteine on Inflammatory Injury Induced by Lipopolysaccharide in Vascular Endothelial Cells

ZHANG Zhenzhen, XIONG Ting, ZHENG Rui, HUANG Jialin, GUO Ling^*

Department of Prosthodontics, Hospital of Stomatology,Southwest Medical University,Luzhou 646000,China

Received:2019-07-01 Online:2020-05-28 Published:2020-05-28

摘要/Abstract

摘要： 目的: 探讨N-乙酰半胱氨酸(NAC)是否对脂多糖(LPS)刺激的血管内皮细胞具有保护作用,为NAC用于治疗种植体周围炎、牙周炎等提供理论基础。方法: 通过细胞计数试剂盒(CCK-8)法检测不同浓度的LPS或NAC对人脐静脉血管内皮细胞(HUVECs)增殖的影响,以获得刺激HUVECs的最适药物浓度。添加最佳药物浓度的LPS和(或)NAC处理HUVECs 24 h,实时半定量聚合酶链反应(RT-qPCR)检测炎症因子白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)和细胞粘附分子-1(ICAM-1)的mRNA表达;酶联免疫吸附试验(ELISA)检测TNF-α和IL-8的蛋白表达;Western blot法检测ICAM-1和NF-κB信号通路的表达情况。结果: LPS刺激HUVECs过量表达炎症因子TNF-α、IL-8和ICAM-1。此外,LPS增加NF-κB通路的磷酸化P65(pP65)表达。然而,NAC预处理HUVECs后,显著抑制了LPS引起的TNF-α、IL-8和ICAM-1表达的增加及降低了pP65的分泌水平。结论: 本结果表明NAC 保护血管内皮细胞免受LPS介导的炎症损伤,从而减轻炎症反应,其潜在的机制可能与NF-κB信号通路有关。

关键词: 血管内皮细胞, N-乙酰半胱氨酸, 脂多糖, 炎症反应, NF-κB信号通路

Abstract: Objective: To investigate whether N-acetylcysteine (NAC) has protective effects on lipopolysaccharide (LPS)-stimulated vascular endothelial cells, and provide a theoretical basis for the treatment of peri-implantitis and periodontitis. Methods: The effects of different concentrations of LPS or NAC on proliferation of human umbilical vein endothelial cells (HUVECs) were measured by cell counting kit 8 (CCK-8) to obtain the optimal drug concentration for stimulation of HUVECs. After adding the optimal drug concentration of LPS and/or NAC to treat HUVECs for 24 h, real-time semi-quantitative polymerase chain reaction (RT-qPCR) was used to detect the mRNA expression of inflammatory factors interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), and cell adhesion molecule 1 (ICAM-1). Protein expression of TNF-α and IL-8 were detected by enzyme-linked immunosorbent assay (ELISA). The protein expressions of ICAM-1 and NF-κB signaling pathway were detected by western blot. Results: LPS stimulated HUVECs to overexpress inflammatory factors TNF-α, IL-8, and ICAM-1. In addition, LPS increased phosphorylation of P65 (pP65) expression in the NF-κB pathway. However, NAC pretreatment of HUVECs significantly inhibited the increase of TNF-α, IL-8, and ICAM-1 expression induced by LPS and decreased the secretion level of pP65. Conclusion: NAC protects HUVECs against LPS-mediated inflammatory reaction and alleviates inflammation. The underlying mechanism is related to the NF-κB pathway.

Key words: vascular endothelial cells, N-acetylcysteine, lipopolysaccharide, inflammatory response, NF-κB signaling pathway

张贞祯, 熊婷, 郑睿, 黄佳琳, 郭玲. N-乙酰半胱氨酸对脂多糖诱导的血管内皮细胞炎症损伤的影响[J]. 口腔医学研究, 2020, 36(4): 377-381.

ZHANG Zhenzhen, XIONG Ting, ZHENG Rui, HUANG Jialin, GUO Ling. Effect of N-acetylcysteine on Inflammatory Injury Induced by Lipopolysaccharide in Vascular Endothelial Cells[J]. Journal of Oral Science Research, 2020, 36(4): 377-381.

参考文献

[1] Ting M, Craig J, Balkin BE, et al. Peri-implantitis: A comprehensive overview of systematic reviews [J]. J Oral Implantol, 2018, 44(3): 225-247.
[2] Lafaurie GI, Sabogal MA, Castillo DM, et al. Microbiome and microbial biofilm profiles of peri-implantitis: A systematic review [J]. J Periodontol, 2017, 88(10): 1066-1089.
[3] 张慧,李汪阳,黄英,等.N-乙酰半胱氨酸对脂多糖诱导的成骨细胞相关基因及蛋白表达影响的研究[J].口腔医学研究,2017,33(11):1151-1155.
[4] Kanmani P, Kim H. Functional capabilities of probiotic strains on attenuation of intestinal epithelial cell inflammatory response induced by TLR4 stimuli [J]. Biofactors, 2018, 45(2):223-235.
[5] Yoo H, Ku SK, Lee T, et al. Orientin inhibits HMGB1-induced inflammatory responses in HUVECs and in murine polymicrobial sepsis [J]. Inflammation, 2014, 37(5): 1705-1717.
[6] Dinicola S, De Grazia S, Carlomagno G, et al. N-acetylcysteine as powerful molecule to destroy bacterial biofilms. A systematic review [J]. Eur Rev Med Pharmacol Sci, 2014, 18(19): 2942-2948.
[7] Mokhtari V, Afsharian P, Shahhoseini M, et al. A review on various uses of N-acetyl cysteine[J]. Cell J, 2017, 19(1):11-17.
[8] Schwarz F, Derks J, Monje A, et al. Peri-implantitis [J]. J Periodontol, 2018, 89 Suppl 1:S267-S290.
[9] Tseng CY, Chang JF, Wang JS, et al. Protective effects of N-acetyl cysteine against diesel exhaust particles-induced intracellular ROS generates pro-inflammatory cytokines to mediate the vascular permeability of capillary-like endothelial tubes [J]. PLoS One, 2015, 10(7): e0131911.
[10] Lin Q, Qin X, Shi M, et al. Schisandrin B inhibits LPS-induced inflammatory response in human umbilical vein endothelial cells by activating Nrf2 [J]. Int Immunopharmacol, 2017, 49:142-147.
[11] Giusti L, Gabriele M, Penno G. A fermented whole grain prevents lipopolysaccharides-induced dysfunction in human endothelial progenitor cells [J]. Oxid Med Cell Longev, 2017, 2017:1026268.
[12] Sun LT, Chen L, Wang F, et al. Exogenous hydrogen sulfide prevents lipopolysaccharide-induced inflammation by blocking the TLR4/NF-κB pathway in MAC-T cells [J]. Gene, 2019, 710:114-121.
[13] Zhang GY, Lu D, Duan SF, et al. Hydrogen sulfide alleviates lipopolysaccharide-induced diaphragm dysfunction in rats by reducing apoptosis and inflammation through ROS/MAPK and TLR4/NF-κB signaling pathways [J]. Oxid Med Cell Longev, 2018, 2018:9647809.
[14] Wu XH, Xu WH, Feng XB, et al. TNF-α mediated inflammatory macrophage polarization contributes to the pathogenesis of steroid-induced osteonecrosis in mice [J]. Int J Immunopathol Pharmacol, 2015, 28(3):351-361.
[15] 吴芸菲,张黎,张旭凤,等.烟草提取物对牙周炎小鼠牙周组织TLR4炎症通路的影响[J].口腔医学研究,2019,35(7):651-656.

N-乙酰半胱氨酸对脂多糖诱导的血管内皮细胞炎症损伤的影响

Effect of N-acetylcysteine on Inflammatory Injury Induced by Lipopolysaccharide in Vascular Endothelial Cells

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