口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (6): 646-649.DOI: 10.13701/j.cnki.kqyxyj.2017.06.017

• 临床研究论著 • 上一篇    下一篇

红芪多糖对脂多糖诱导的牙周膜细胞凋亡及对Wnt信号通路的影响

徐明1*, 杨建斌2   

  1. 1. 信阳职业技术学院附属医院口腔科 河南 信阳 464000;
    2. 新乡医学院第一附属医院口腔科 河南 新乡 453000
  • 收稿日期:2017-01-06 出版日期:2017-06-20 发布日期:2017-06-26
  • 通讯作者: 徐明,电话:13507600979
  • 作者简介:徐明(1973~ ),男,河南信阳人,硕士在读,副主任医师,主要从事口腔医学的研究工作。
  • 基金资助:
    河南省医学科技攻关计划项目(编号:201403185)

Effect of Hedysari Polysaccharide on Apoptosis of Periodontal Ligament Cells Induced by LPS and the Expression of Wnt Signaling Pathway.

XU Ming1*, YANG Jian-bin2.   

  1. 1. Department of Stomatology, Affiliated Hospital of Xinyang Polytechnic, Xinyang 464000, China;
    2. Department of Stomatology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453000, China.
  • Received:2017-01-06 Online:2017-06-20 Published:2017-06-26

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摘要： 目的:探讨红芪多糖对脂多糖(LPS)诱导的牙周膜细胞凋亡及对Wnt信号通路的影响。方法:体外分离培养人牙周膜细胞。实验分为3组,依次为对照组、脂多糖组和红芪多糖组。对照组细胞用正常的细胞培养液培养细胞;脂多糖组和红芪多糖组细胞用含有脂多糖浓度为10 mg/L的培养液培养细胞;红芪多糖组细胞培养液中加入红芪多糖终浓度为10 mg/L的培养液。流式细胞仪检测细胞凋亡情况,探针法检测细胞内的活性氧(ROS)水平。试剂盒检测细胞中碱性磷酸酶活性和分泌的肿瘤坏死因子-α(TNF-α)水平。Western blot检测细胞中c-myc、β-连环蛋白(β-catenin)、Bcl-2相关X蛋白(Bax)单克隆抗体、B细胞淋巴瘤/白血病-2(Bcl-2)蛋白水平。结果:脂多糖组细胞存活率明显低于对照组(P<0.01)。脂多糖组细胞凋亡率和Bax水平、ROS水平明显高于对照组(P<0.01)。红芪多糖组细胞凋亡率和Bax水平、ROS水平明显低于脂多糖组(P<0.01)。脂多糖组细胞Bcl-2、c-myc、β-catenin水平和碱性磷酸酶活性明显低于对照组(P<0.01)。红芪多糖组细胞Bcl-2、c-myc、β-catenin水平和碱性磷酸酶活性明显高于脂多糖组(P<0.01)。脂多糖组细胞分泌的TNF-α水平明显高于对照组(P<0.01)。红芪多糖组细胞分泌的TNF-α水平明显低于脂多糖组(P<0.01)。结论:红芪多糖能够抑制脂多糖诱导的人牙周膜细胞凋亡,抑制细胞分泌TNF-α,作用机制可与Wnt信号通路、ROS水平有关。

关键词: 人牙周膜细胞, 脂多糖, 红芪多糖, 凋亡

Abstract: Objective: To study the effect of Hedysari polysaccharide on apoptosis of periodontal ligament cells induced by LPS and the expression of Wnt signaling pathway. Methods: Human periodontal ligament cells were cultured and divided into three groups, control group, LPS group with normal cell culture medium containing 10μg/mL LPS and Hedysari polysaccharide group with 10μg/mL LPS and 10μg/mL Hedysari polysaccharide. Cell apoptosis was detected by flow cytometry. Intracellular ROS levels were detected by the probe method. The activity of alkaline phosphatase and the levels of TNF-α in the cells were detected by ELISA. The levels of c-myc, β-catenin, Bax and Bcl-2 were detected by Western Blot. Results: The apoptosis rate, expression of Bax and ROS level in the LPS group were significantly higher than those in the control group and lower in Hedysari polysaccharide group (P<0.01). The levels of Bcl-2, c-myc, β-catenin and alkaline phosphatase in the LPS group were significantly lower than those in the control group and significantly higher in Hedysari polysaccharide group (P<0.01). The level of TNF-α in LPS group was significantly higher than those in the control group and Hedysari polysaccharide group (P<0.01). Conclusion: Hedysari polysaccharide can inhibit LPS induced apoptosis and the secretion of TNF-α of human periodontal ligament cells. The mechanism may relate to Wnt signaling pathway and ROS level.

Key words: Human periodontal ligament cellsLipopolysaccharide , Radix , Hedysari polysaccharideApoptosis

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