周期性张应力对牙周膜细胞内LIF和LIFR的表达影响

doi:10.13701/j.cnki.kqyxyj.2019.09.011

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (9): 863-867.DOI: 10.13701/j.cnki.kqyxyj.2019.09.011

周期性张应力对牙周膜细胞内LIF和LIFR的表达影响

梁又德¹, 韦伟¹, 薛伟伟¹, 周瑞平¹, 傅润英¹, 王贻宁^2*

1. 深圳市第七人民医院口腔中心广东深圳 518081;
2. 武汉大学口腔医学院修复科湖北武汉 430079

收稿日期:2018-07-06 出版日期:2019-09-28 发布日期:2019-09-25
通讯作者: 王贻宁,E-mail:yiningwang@163.com
作者简介:梁又德(1982~ ),女,湖南人,博士,副主任医师,研究方向:口腔种植修复。

Effect of Cyclic-tension Strain on mRNA Levels of Leukemia Inhibitory Factor and Leukemia Inhibitory Factor Receptor in Human Periodontal Ligament Cells

LIANG Youde¹, WEI Wei¹, XUE Weiwei¹, ZHOU Ruiping¹, FU Runying¹, WANG Yining^2*

1. Department of Dentistry, Seventh People's Hospital of Shenzhen,Shenzhen 518052, China;
2. Department of Prosthodontics, School of Stomatology, Wuhan University, Wuhan 430079, China.

Received:2018-07-06 Online:2019-09-28 Published:2019-09-25

摘要/Abstract

摘要： 目的:观察周期性张应力作用下牙周膜细胞(human periodontal ligament cells,HPDLC)中白血病抑制因子(leukemia inhibitory factor,LIF)、白血病抑制因子受体(leukemia inhibitory factor receptor,LIFR) 及碱性磷酸酶(alkaline phosphatase,ALP)的表达变化。方法:通过建立HPDLC体外应力加载系统,对培养在弹性膜6孔板的HPDLC施加0.1 Hz,硅胶膜形变率为18%的周期性张应力,分别在加载48、72 h后利用定量逆转录聚合酶链反应 ( qRT-PCR) 检测HPDLC的LIF,LIFR和ALP的表达变化。结果:HPDLC在加载周期性牵张力后,细胞生长方向顺应力方向而发生改变,LIF和LIFR的表达明显增加,ALP的表达明显减低。结论:周期性循环张力可诱导 HPDLC 中LIF,LIFR表达增强,为LIF,LIFR可能参与正畸力下牙周组织的改建提供依据。

关键词: 人牙周膜细胞, 周期性张应力, 白血病抑制因子

Abstract: Objective: To investigate the effects of cyclic-tension strain on the expression of LIF and LIFR in human periodontal ligament cells (HPDLC), and to illustrate the molecular mechanism underlying orthodontic periodontal tissue remodeling. Methods: In this study, HPDLC were subjected to 18% elongation by strain unit at 0.1Hz for 48h and 72h. The expressions of LIF, LIFR, and ALP mRNA were measured by real-time quantitative RT-PCR. Morphological changes were observed after mechanical stress. Results: Cells inclined parallel to each other and aligned their long axis perpendicular to the stretching force vector. The status was more and more transparent with extension of time. qRT-PCR showed that mRNA expression of LIF, LIFR, and ALP changed upon the stimulation. The expressions of LIF and LIFR were increased dramatically (P<0.01) and the expression of ALP was decreased significantly (P<0.01) after 72h. Conclusion: LIF and LIFR may play an role in periodontal tissue remodeling induced by orthodontic therapy.

Key words: human periodontal ligament cells, cyclic-tension strain, leukemia inhibitory factor

梁又德, 韦伟, 薛伟伟, 周瑞平, 傅润英, 王贻宁. 周期性张应力对牙周膜细胞内LIF和LIFR的表达影响[J]. 口腔医学研究, 2019, 35(9): 863-867.

LIANG Youde, WEI Wei, XUE Weiwei, ZHOU Ruiping, FU Runying, WANG Yining. Effect of Cyclic-tension Strain on mRNA Levels of Leukemia Inhibitory Factor and Leukemia Inhibitory Factor Receptor in Human Periodontal Ligament Cells[J]. Journal of Oral Science Research, 2019, 35(9): 863-867.

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周期性张应力对牙周膜细胞内LIF和LIFR的表达影响

Effect of Cyclic-tension Strain on mRNA Levels of Leukemia Inhibitory Factor and Leukemia Inhibitory Factor Receptor in Human Periodontal Ligament Cells

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