口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (10): 1089-1092.DOI: 10.13701/j.cnki.kqyxyj.2018.10.014

• 牙周病学研究 • 上一篇    下一篇

TP对脂多糖介导人牙周膜成纤维细胞细胞间黏附分子-1表达的影响

范芹1,3, 管晓燕2,3*, 李小娜3,4, 刘建国3,4   

  1. 1. 遵义医科大学附属口腔医院口腔种植科 贵州 遵义 563099;
    2. 遵义医科大学附属口腔医院正畸科 贵州 遵义 563099;
    3. 遵义医科大学口腔医学院 贵州 遵义 563006;
    4. 贵州省高等学校口腔疾病研究特色重点实验室暨遵义市口腔疾病研究重点实验室 贵州 遵义 563006
  • 收稿日期:2018-06-07 出版日期:2018-10-28 发布日期:2018-10-24
  • 通讯作者: 管晓燕,E-mail:1278279125@qq.com
  • 作者简介:范芹(1976~ ),女,贵州遵义人,副教授,硕士,主要从事口腔种植及牙周病学的研究。
  • 基金资助:
    贵州省高等学校重点学科建设项目(黔学位合字ZDXK[2017]5号),贵州省市科技合作项目(省市科合[2014]41号),遵义市科技局项目(遵市科[2016]18号)

Effect of Tea Polyphenols on ICAM-1 Expression in LPS-mediated Human Periodontal Ligament Fibroblast Cells.

FAN Qin1,3, GUAN Xiao-yan2,3*, LI Xiao-na3,4, LIU Jian-guo3,4.   

  1. 1. Department of Implantolgy, Hospital of Stomatology, Zunyi Medical University, Zunyi 563099, China;
    2. Department of Orthodontics, Hospital of Stomatology, Zunyi Medical University, Zunyi 563099, China;
    3.School of Stomatology, Zunyi Medical University, Zunyi 563006, China;
    4. The Special Key Laboratory of Oral Diseases Research, Institution of Higher Education in Guizhou Province, the Key Laboratory of Oral Diseases Research of Zunyi City, Zunyi 563006, China.
  • Received:2018-06-07 Online:2018-10-28 Published:2018-10-24

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摘要： 目的: 检测人牙周膜成纤维细胞(human periodontal ligament fibroblasts,HPDLF)在脂多糖(lipopolysaccharide,LPS)介导下细胞间黏附分子-1﹙intercellular adhesion molecule-1,ICAM-1﹚的表达,探讨TP(tea polyphenols,TP)对其表达的影响。方法: HPDLF采用改良组织块法体外培养,将HPDLF随机分成LPS组、TP100组、TP200组,用酶联免疫吸附试验法(enzyme linked immuosorbent,ELISA)检测不同浓度TP对LPS介导下HPDLF分泌ICAM-1的活性。实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,Real-time PCR)检测ICAM-1 mRNA的表达。结果: 在LPS干预下,不同时间不同浓度的TP影响下均可抑制ICAM-1分泌,其活性降低,与LPS组比较差异有统计学意义(P<0.05);且ICAM-1 mRNA表达水平显著降低,与LPS组比较差异有统计学意义(P<0.05)。结论: TP对ICAM-1的抑制作用明显,100 μg/mL TP对其的抑制作用更显著,提示TP的抗炎机制可能与抑制ICAM-1有关。

关键词: TP, 脂多糖, 人牙周膜细胞, 细胞间黏附分子-1

Abstract: Objective: To investigate the effect of tea polyphenols (TP) on ICAM-1 expression in LPS-treated human periodontal ligament fibroblast cells (HPDLFs). Methods: HPDLFs were obtained from periodontal tissues by modified-cultured method. After induced by LPS, the control group was only treated with LPS, the experiment groups were treated with 100 μg/mL TP (TP100 group) or 200 μg/mL TP (TP200 group). The levels of ICAM-1 secretion in three groups were tested by ELISA. And the mRNA expression of ICAM-1 was detected by real-time PCR. Results: ICAM-1 was inhibited by TP after induced by LPS, and its activity was decreased significantly (P<0.05). The ICAM-1 mRNA levels of TP treated groups were significantly lower than that of LPS group (P<0.05). Conclusion: TP inhibited the expression of ICAM-1 and the effect was more significantly at 100 μg/mL. This result suggested that the anti-inflammatory mechanism of TP might relate to the inhibition of ICAM-1.

Key words: Tea polyphenols, Lipopolysaccharide, Human periodontal ligament cells, Intercellular adhesion molecule-1