口腔医学研究

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (12): 1177-1181.DOI: 10.13701/j.cnki.kqyxyj.2019.12.019

• 牙周病学研究 • 上一篇    下一篇

低频磁刺激对高糖诱导人牙周膜细胞损伤的保护作用

郑广明1*, 张健2, 张文怡3   

  1. 1. 天津市津南医院口腔科 天津 300350;
    2. 天津医科大学附属口腔医院口腔颌面外科 天津 300070;
    3. 天津医科大学附属口腔医院修复科 天津 300070
  • 收稿日期:2019-03-27 出版日期:2019-12-28 发布日期:2019-12-23
  • 通讯作者: 郑广明,E-mail:13702107050@163.com
  • 作者简介:郑广明(1984~ ),男,吉林人,学士,主治医师,主要从事口腔科临床治疗工作。

Protective Effect of Low-frequency Magnetic Stimulation on Human Periodontal Ligament Cells Injured by High Glucose

ZHENG Guangming1*, ZHANG Jian2, ZHANG Wenyi3   

  1. 1. Department of Stomatology, Tianjin Xianshui Hospital, Tianjin 300350, China;
    2. Department of Oral and Maxillofacial Surgery, Stomatological Hospital Affiliated to Tianjin Medical University, Tianjin 300070, China;
    3. Department of Prosthodontics, Stomatological Hospital Affiliated to Tianjin Medical University, Tianjin 300070, China.
  • Received:2019-03-27 Online:2019-12-28 Published:2019-12-23

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摘要： 目的:探讨低频磁刺激对高糖介导人牙周膜细胞损伤的保护作用。方法:体外复苏培养人牙周膜细胞,随机分为正常对照组和高糖模型组以及低频磁刺激组(3个亚组)共5个实验组。流式细胞术的方法检测细胞周期的变化,TUNEL法检测细胞的凋亡情况,比色法检测Caspase-3、6、9活性,逆转录PCR及Western-blot检测CXCL1、CXCL2及其受体CXCR2基因和蛋白的表达水平。结果:与正常对照组比较,高糖模型中人牙周膜细胞凋亡增加;Caspase-3、6、9活性均得到了显著提高(P<0.05),CXCL1、CXCL2及其受体CXCR2基因和蛋白的表达显著降低(P<0.05)。与高糖模型组比较,低频磁刺激组细胞凋亡率降低,Caspase-3、6、9活性降低(P<0.05),CXCL1、CXCL2及其受体CXCR2基因和蛋白的表达上调(P<0.05);对细胞周期的影响不明显(P>0.05),3个亚组比较时,刺激强度为1.5 T时以上变化最明显(P<0.05)。结论:高糖条件促进人牙周膜细胞凋亡,低频磁刺激对高糖诱导人牙周膜细胞损伤有保护作用。

关键词: 低频磁刺激, 人牙周膜细胞, 高糖, 损伤

Abstract: Objective: To investigate the protective effect of low-frequency magnetic stimulation on periodontal membrane injury mediated by high sugar. Methods: Human periodontal membrane cells were cultured by resuscitation in vitro, which were randomly divided into normal control group, high sugar model group, and low-frequency magnetic stimulation group (3 subgroups). Flow cytometry method was used to detect the changes in cell cycle. TUNEL method was used to detect apoptosis. Colorimetric method was used to detect Caspase-3, 6, and 9 activities, and reverse transcription PCR and Western-blot were used to detect CXCL1, CXCL2, and its receptor CXCR2 gene and protein expression levels. Results: Compared with the normal control group, the apoptosis of human periodontal membrane cells increased in the high sugar model. Caspase-3, 6, and 9 activities were all improved (P<0.05). The expressions of CXCL1, CXCL2, and its receptor CXCR2 genes and proteins were significantly reduced (P<0.05). Compared with the high-sugar model group, the cell apoptosis rate in the low-frequency magnetic stimulation group decreased, and the Caspase-3, 6, and 9 activities decreased (P<0.05). CXCL1, CXCL2, and its receptor CXCR2 gene and protein expression increased (P<0.05). The effect on the cell cycle was not obvious (P>0.05). When comparing three subgroups, the change in stimulus intensity above 1.5T was the most obvious (P<0.05). Conclusion: High sugar conditions lead to apoptosis of human periodontal membrane cells, and low-frequency magnetic stimulation has protective effect on human periodontal membrane cell injury induced by high sugar.

Key words: low-frequency magnetic stimulation, human periodontal ligament cells, high glucose, injury