Nrf2在牙周炎诱发大鼠肝损伤中的作用研究

doi:10.13701/j.cnki.kqyxyj.2020.12.008

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (12): 1113-1116.DOI: 10.13701/j.cnki.kqyxyj.2020.12.008

Nrf2在牙周炎诱发大鼠肝损伤中的作用研究

李艳¹, 夏博园¹, 李鑫¹, 丁旭¹, 王闻天^2*, 于维先^3,4*

1.吉林大学口腔医院牙周病科吉林长春 130021;
2.航天中心医院口腔科吉林长春 100049;
3.吉林大学口腔医院老年口腔科吉林长春 130021;
4.吉林省牙发育及颌骨重塑与再生重点实验室吉林长春 130021

收稿日期:2020-07-08 出版日期:2020-12-28 发布日期:2020-12-28
通讯作者: *王闻天,E-mail:153731218@qq.com;于维先,E-mail:yu-wei-xian@163.com
作者简介:李艳(1994~ ),女,山东人,硕士,医师,主要从事牙周免疫炎症的研究。
基金资助:
吉林省财政厅科技项目(编号:JCSZ2019378-1);吉林省科技厅自然科学基金(编号:20190201058JC);吉林省科技厅国际合作项目(编号:20180414053GH)

Role of Nrf2 in Liver Injury Induced by Periodontitis in Rats

LI Yan¹, XIA Boyuan¹, LI Xin¹, DING Xu¹, WANG Wentian^2*, YU Weixian^3,4*

1. Department of Periodontics, Hospital of Stomatology, Jilin University, Changchun 130021, China;
2. Department of Stomatology, Aero Space Center Hospital, Beijing 100049, China;
3. Department of Geriatric Stomatology, Hospital of Stomatology, Jilin University, Changchun 130021, China;
4. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Hospital of Stomatology, Jilin University, Changchun 130021, China

Received:2020-07-08 Online:2020-12-28 Published:2020-12-28

摘要/Abstract

摘要： 目的:初步探讨核因子E2相关因子2(nuclear factor erythroid 2 related factor 2, Nrf2)在牙周炎诱发大鼠肝损伤中的作用。方法:将24只雄性Wistar大鼠随机分成正常组和牙周炎组,每组各12只。采用0.2 mm正畸丝结扎大鼠单侧上颌第一磨牙牙颈部并接种牙龈卟啉单胞菌(Porphyromonas gingival, Pg)的方法建立牙周炎模型。建模6周后,10%水合氯醛麻醉下记录牙龈出血指数、牙周探诊深度、牙齿松动度;采用组织病理学、免疫组织化学方法和实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR)分别检测肝损伤情况及肝组织中Nrf2、Kelch样环氧氯丙烷相关蛋白1(Kelch-like ECH-associated protein 1, Keap1)和醌氧化还原酶-1(NAD(P)H: quinone oxidoreductase-1, NQO-1)的mRNA和蛋白的表达。结果:牙周炎组大鼠牙周组织出现明显炎症反应;牙周炎大鼠肝组织内肝索结构紊乱,肝细胞变性、坏死;Nrf2、NQO-1的mRNA表达水平低于正常组,Keap1高于正常组;牙周炎大鼠Nrf2蛋白表达水平低于正常组。结论:Nrf2在牙周炎诱发大鼠肝损伤中发挥着重要作用。

关键词: 牙周炎, 肝损伤, 核因子E2相关因子2

Abstract: Objective: To investigate the role of Nrf2 in liver injury induced by periodontitis in rats. Methods: 24 male Wistar rats were randomly divided into normal group and periodontitis group, with 12 rats in each group. The periodontitis model was established by 0.2mm orthodontic wire ligation and inoculation of Porphyromonas gingival on the neck of the first maxillary molar. After 6 weeks of modeling, periodontal clinical parameters were measured. Histopathological and immunohistochemical methods and qRT-PCR were used to detect the liver injury and the expression of Nrf2, Keap1, and NQO-1 mRNA and protein in liver tissues. Results: The periodontal tissues of rats in the periodontitis group showed obvious inflammation. The liver cord structure was disorder. Hepatocytes were degenerated and necrosis. mRNA expression levels of Nrf2 and NQO-1 were lower than that of the normal group, and Keap1 was higher than that of the normal group. The Nrf2 protein expression level in periodontitis group was lower than that in normal group. Conclusion: Nrf2 plays an important role in liver injury induced by periodontitis in rats.

Key words: periodontitis, liver injury, Nrf2

李艳, 夏博园, 李鑫, 丁旭, 王闻天, 于维先. Nrf2在牙周炎诱发大鼠肝损伤中的作用研究[J]. 口腔医学研究, 2020, 36(12): 1113-1116.

LI Yan, XIA Boyuan, LI Xin, DING Xu, WANG Wentian, YU Weixian. Role of Nrf2 in Liver Injury Induced by Periodontitis in Rats[J]. Journal of Oral Science Research, 2020, 36(12): 1113-1116.

参考文献

[1] Romandini M, Laforì A, Romandini P, et al. Periodontitis and platelet count: A new potential link with cardiovascular and other systemic inflammatory diseases [J]. J Clin Periodontol, 2018, 45(11):1299-1310.
[2] Vasconcelos ACCG, Vasconcelos DFP, Pereira da Silva FR, et al. Periodontitis causes abnormalities in the liver of rats [J]. J Periodontol, 2019, 90(3):295-305.
[3] Chen M, Cai W, Zhao S, et al. Oxidative stress-related biomarkers in saliva and gingival crevicular fluid associated with chronic periodontitis: A systematic review and meta-analysis [J]. J Clin Periodontol, 2019, 46(6):608-622.
[4] Li X, Sun X, Zhang X, et al. Enhanced oxidative damage and Nrf2 downregulation contribute to the aggravation of periodontitis by diabetes mellitus [J]. Oxid Med Cell Longev, 2018, 2018:9421019.
[5] Ding Y, Ren J, Yu H, et al. Porphyromonas gingivalis, a periodontitis causing bacterium, induces memory impairment and age-dependent neuroinflammation in mice [J]. Immun Ageing, 2018, 15:6.
[6] Mester A, Ciobanu L, Taulescu M, et al. Periodontal disease may induce liver fibrosis in an experimental study on Wistar rats [J]. J Periodontol, 2019, 90(8):911- 919.
[7] Tu W, Wang H, Li S, et al. The anti-inflammatory and anti-oxidant mechanisms of the Keap1/Nrf2/ARE signaling pathway in chronic diseases [J]. Aging Dis, 2019, 10(3):637-651.
[8] Du L, Wang L, Wang B, et al. A novel compound AB38b attenuates oxidative stress and ECM protein accumulation in kidneys of diabetic mice through modulation of Keap1/Nrf2 signaling [J]. Acta Pharmacol Sin, 2020, 41(3):358-372.
[9] Funakoshi-Tago M, Nonaka Y, Tago K, et al. Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2 [J]. Sci Rep, 2020, 10(1):2584.
[10] Li Y, Zeng Y, Huang Q, et al. Helenalin from centipeda minima ameliorates acute hepatic injury by protecting mitochondria function, activating Nrf2 pathway and inhibiting NF-κB activation [J]. Biomed Pharmacother, 2019, 119:109435.
[11] 岳轶云,丁小函,李艳.Toll样受体4在慢性牙周炎诱发大鼠肝脏炎症中的作用[J].口腔医学研究,2019,36(2):139-142.

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Nrf2在牙周炎诱发大鼠肝损伤中的作用研究

Role of Nrf2 in Liver Injury Induced by Periodontitis in Rats

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