口腔医学研究

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (2): 139-142.DOI: 10.13701/j.cnki.kqyxyj.2020.02.012

• 牙周病学研究 • 上一篇    下一篇

Toll样受体4在慢性牙周炎诱发大鼠肝脏炎症中的作用

岳轶云1, 丁小函1, 李艳1, 夏博园1, 王闻天2*, 于维先3,4*   

  1. 1. 吉林大学口腔医学院牙周病科 吉林 长春 130021;
    2. 航天中心医院口腔科 北京 100049;
    3. 吉林大学口腔医学院老年口腔科 吉林 长春 130021;
    4. 吉林省牙发育及颌骨重塑与再生重点实验室 吉林 长春 130021
  • 收稿日期:2019-06-13 出版日期:2020-02-28 发布日期:2020-04-24
  • 通讯作者: 王闻天,E-mail:153731218@qq.com; 于维先,E-mail:yu-wei-xian@163.com
  • 作者简介:岳轶云(1992~ ),女,内蒙古人,硕士,医师,主要从事牙周免疫炎症的研究。
  • 基金资助:
    吉林省科技厅自然科学基金(编号:20190201058JC);吉林省科技厅国际合作项目(编号:20180414053GH)

Role of TLR4 in Liver Inflammation Induced by Chronic Periodontitis in Rats

YUE Yiyun1, DING Xiaohan1, LI Yan1, XIA Boyuan1, WANG Wentian2*, YU Weixian3,4*   

  1. 1. Department of Periodontics, School of Stomatology, Jilin University, Changchun 130021, China;
    2. Department of Stomatology, Aero Space Center Hospital, Beijing 100049, China;
    3. Department of Geriatric Stomatology, School of Stomatology, Jilin University, Changchun 130021, China;
    4. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, School of Stomatology, Jilin University, Changchun 130021, China
  • Received:2019-06-13 Online:2020-02-28 Published:2020-04-24

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摘要： 目的:初步探究Toll样受体4(Toll like receptor 4, TLR4)在慢性牙周炎诱发大鼠肝脏炎症中的作用。方法:将24只雄性Wistar大鼠随机分成两组,对照组和牙周炎组每组各12只,2%戊巴比妥钠麻醉下检测牙龈出血指数、牙周探诊深度及松动度;将20 μL牙龈卟啉单胞菌脂多糖(Porphyromonas gingivalis lipopolysaccharide, Pg-LPS)(1 g/L)注射到大鼠双侧上颌第一磨牙龈沟及第一二磨牙龈间隙内,隔1 d注射1次,每周3次,连续6周。对照组不做处理。最后一次注射Pg-LPS 12 h后,2%戊巴比妥钠麻醉下检测牙周探诊深度、牙龈出血指数及松动度等3项牙周指标后将大鼠处死,取其上颌骨及肝脏,采用组织学方法、实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction, qRT-PCR)、Western blot分别检测肝组织炎症反应情况及TLR4、肿瘤坏死因子-α(tumor necrosis factor, TNF-α)和白细胞介素-6(interleukin-6, IL-6)的基因和蛋白表达水平。结果:牙周炎组大鼠牙周探诊深度、牙龈出血指数及松动度均重于对照组;苏木精-伊红(hematoxylin-eosin, HE)染色结果显示慢性牙周炎大鼠肝组织内肝索结构紊乱,汇管区可见大量淋巴细胞浸润,大量肝细胞呈空泡样改变;qRT-PCR结果显示慢性牙周炎组大鼠肝组织TLR4、TNF-α和IL-6的mRNA表达水平较对照组升高;Western blot结果显示TLR4蛋白表达与基因表达一致。结论:TLR4在慢性牙周炎诱发大鼠肝脏炎症性改变的过程中发挥着重要的作用。

关键词: 慢性牙周炎, 肝脏炎症, Toll样受体4

Abstract: Objective:To investigate the role of TLR4 in liver inflammation induced by chronic periodontitis in rats. Methods:Twenty-four male Wistar rats were randomly euqally divided into two groups. In the periodontitis group, 20 μL Pg-LPS (1 g/L) was injected into the first and second gingival furrow of the maxilla on both sides of the rats, once every other day, three times a week, for 6 weeks. The control group was not treated. 12 h after the last injection of Pg-LPS, rats were sacrificed and the maxilla and liver were extracted. Histological method was used to detect the inflammatory response of the liver. qRT-PCR and Western blotting were used to detect the expression of TLR4, TNF-α, and IL-6. Results:HE staining showed that the hepatic cord was disorganized in the liver tissue of rats with chronic periodontitis, infiltrated by inflammatory cells such as neutrophils, and a large number of liver cells presented vacuolar changes. qRT-PCR results showed that the expression levels of TLR4, TNF-α, and IL-6 in the liver of rats in the chronic periodontitis group were higher than those in the control group. Western blot result of TLR4 was consistent with the gene expression. Conclusion:TLR4 plays an important role in the inflammatory changes of liver induced by chronic periodontitis in rats.

Key words: chronic periodontitis, liver inflammation, Toll like receptor 4