慢性牙周炎患者血清中TGF-β各亚型的定量分析

doi:10.13701/j.cnki.kqyxyj.2017.06.010

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (6): 618-622.DOI: 10.13701/j.cnki.kqyxyj.2017.06.010

慢性牙周炎患者血清中TGF-β各亚型的定量分析

李爽¹, 孟晓雪², 周建业³, 易根云¹, 黄慧敏¹, 张小凤¹, 李志强^3*, 余占海^1*

1. 兰州大学口腔医学院甘肃兰州 730000;
2. 兰州大学第一医院甘肃兰州 730000;
3. 西北民族大学口腔医学院甘肃兰州 730000)

收稿日期:2016-11-23 出版日期:2017-06-20 发布日期:2017-06-26

Quantitative Analysis of Serum TGF-β Isoforms in Chronic Periodontitis Patients.

LI Shuang¹, MENG Xiao-Xue² , ZHOU Jian-Ye³, YI Gen-Yun¹ , HUANG Hui-Min¹, ZHANG Xiao-Feng¹, LI Zhi-Qiang^3*, YU ZHAN Hai^1*.

1. School of Stomatology, Lanzhou University, Lanzhou 730000, China;
2. The First Hospital of Lanzhou University, Lanzhou 730000, China;
3. School of Stomatology, Northwest University for Nationalities, Lanzhou 730000, China.

Received:2016-11-23 Online:2017-06-20 Published:2017-06-26

摘要/Abstract

摘要： 目的:检测慢性牙周炎患者血清中TGF-β各亚型(TGF-β1、TGF-β2、TGF-β3)的表达差异,探讨三者在慢性牙周炎中的作用。方法:研究对象分为中度牙周炎组(n=9)、重度牙周炎组(n=18)和健康对照组(n=19),基于Lumninex 200多重分析仪对其血清进行定量分析。结果:对照组血清中TGF-β1浓度(41053.26±8318.65) ng/L及TGF-β3浓度(2454.58±552.73) ng/L含量水平均高于中度牙周炎组分别为(32112.22±7153.87) ng/L,P＜0.01;(1991.11±532.22) ng/L,P＜0.05)和重度牙周炎组分别为(30652.89±8245.31) ng/L,P＜0.01;(1829.17±483.68) ng/L,P＜0.01);TGF-β1和TGF-β3分别与CAL、PD、BOP、PLI呈明显负相关;TGF-β1与TGF-β3呈明显正相关。结论:血清中浓度较高的TGF-β1可能对慢性牙周炎的发生发展起一定的抑制作用,而TGF-β3不仅对慢性牙周炎的发生发展起一定的抑制作用而且可能与TGF-β1存在协同作用。

关键词: 慢性牙周炎, 血清, Luminex多重分析, TGF-β亚型

Abstract: Objective: To examine the role of TGF-β isoforms (TGF-β1, TGF-β2 and TGF-β3) in chronic periodontitis. Methods: The research subjects were divided into moderate periodontitis group (n=9), severity periodontitis group (n=18), and healthy controls (n=19). Then their serum was detected through Luminex200 based multi-analytic assay. Results: The levels of serum TGF-β1 (41053.26±8318.65)ng/L and TGF-β3(2454.58±552.73)ng/L in control group were significantly higher than those in moderate periodontitis group (32112.22±7153.87)ng/L, P＜0.01; (1991.11±532.22)ng/L, P＜0.05, respectively and severity periodontitis group (30652.89±8245.31)ng/L, P＜0.01; (1829.17±483.68)ng/L, P＜0.01. TGF-β1 and TGF-β3 were negative correlated with CAL, PD, BOP and PLI. There was a strong positive correlation between TGF-β1 and TGF-β3. Conclusion: Higher concentration of TGF-β1 in serum may have inhibitory effect on the development of chronic periodontitis. TGF-β3 also has a similar inhibitory effect and it maybe interact with TGF-β1.

Key words: Chronic periodontitis , Serum , Luminex multi-analytic assay, TGF-β isoforms

中图分类号:

R781.4

李爽, 孟晓雪, 周建业, 易根云, 黄慧敏, 张小凤, 李志强, 余占海. 慢性牙周炎患者血清中TGF-β各亚型的定量分析[J]. 口腔医学研究, 2017, 33(6): 618-622.

LI Shuang, MENG Xiao-Xue , ZHOU Jian-Ye, YI Gen-Yun , HUANG Hui-Min, ZHANG Xiao-Feng, LI Zhi-Qiang, YU ZHAN Hai.. Quantitative Analysis of Serum TGF-β Isoforms in Chronic Periodontitis Patients.[J]. Journal of Oral Science Research, 2017, 33(6): 618-622.

参考文献

[1] Darveau RP. Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microbiol,2010, 8, 481-490
[2] 李春年,武明轩,陈瑞雪,等.CRP、ICAM-1、E-selectins在实验性牙周炎大鼠牙周组织中的表达及意义[J].口腔医学研究,2015,31(2)∶109-112
[3] 林梅,张冬雪,刘志强,等.慢性牙周炎患者血清IL-33、TNF-α、IL-6的检测及意义[J].首都医科大学学报,2016,37(3)∶255-259
[4] Flanders K C, Yang Y A, Herrmann M, et al. Quantitation of TGF-β proteins in mouse tissues shows reciprocal changes in TGF-β1 and TGF-β3 in normal vs neoplastic mammary epithelium [J]. Oncotarget, 2016,59(2)∶387-392
[5] Dutzan N, Vernal R, Vaque J P, et al.Interleukin-21 expressionand its association with pro-inflammatory cytokines in untreated chronicperiodontitis patients [J]. J Periodontol, 2012,83(7)∶948-954
[6] Khalaf H, Lnn J, BengtssonT.Cytokines and chemokines are differentially expressed in patientswith periodontitis:Possible role for TGF-β1 as a marker for disease progression [J]. Cytokine, 2014, 67(1)∶29-35
[7] GurkanA, Emingil G, Cinareik S, etal. Gingival crevicular fluid transforming growth factor-beta 1 in several forms of periodontal disease [J]. Arch Oral Biol, 2006, 51(10)∶906-912
[8] Armitage GC, Wu Y, Wang HY, et a1. Low Prevalence of a Periodontitis associated interleukin －1 composite genotype in individuals of Chinese heritage [J]. J Periodontol, 2000, 71(2)∶164-171
[9] 张小恒, 余占海, 张国英,等. 转化生长因子-β1对大鼠牙周组织中白细胞介素-6、骨钙素水平的影响[J].口腔医学研究,2010,26(2)∶171-174
[10] Okui T, Aoki Y, Ito H, et al. The presence of IL-17+/FOXP3+ double-positive cells in periodontitis [J]. Journal of Dental Research, 2012, 91(6)∶574-579
[11] 曹雪涛.医学免疫学[J].2013
[12] ZHOU L, LOPES JE, CHONG MM et al. TGF-beta-induced Foxp3 inhibits TH17 cell differentiation by antagonizing RORgammatfunction [J]. Nature 2008; 453∶236-240
[13] Ripamonti U, Parak R, Klar R M, et al. Cementogenesis and osteogenesis in periodontal tissue regeneration by recombinant human transforming growth factor-β3 : A pilot study in Papio ursinus [J]. Journal of Clinical Periodontology, 2016-2020
[14] David L, Mallet C, Mazerbourg S, et al. Identification of BMP9 and BMP10 as functional activators of the orphan activin receptor-like kinase 1 (ALK1) in endothelial cells [J]. Blood, 2007, 109(5)∶1953-1961
[15] Ripamonti U, Parak R, Klar R M, et al. The synergistic induction of bone formation by the osteogenic proteins of the TGF-β supergene family [J]. Biomaterials, 2016, 104∶279-296

慢性牙周炎患者血清中TGF-β各亚型的定量分析

Quantitative Analysis of Serum TGF-β Isoforms in Chronic Periodontitis Patients.

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