口腔医学研究

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (7): 651-656.DOI: 10.13701/j.cnki.kqyxyj.2019.07.009

• 牙周病学研究 • 上一篇    下一篇

烟草提取物对牙周炎小鼠牙周组织TLR4炎症通路的影响

吴芸菲1, 张黎2, 张旭凤2, 符起亚2*   

  1. 1. 海南医学院第一附属医院口腔科 海南 海口 570102;
    2. 海南医学院口腔医学院 海南 海口 570102
  • 收稿日期:2018-10-28 出版日期:2019-07-25 发布日期:2019-07-24
  • 通讯作者: 符起亚,E-mail:283172396@qq.com
  • 作者简介:吴芸菲(1981~ ),女,学士,主治医师,主要从事口腔内科学研究工作
  • 基金资助:
    海南省高等学校科学研究项目(编号:Hnky2018ZD-7)

Effects of Tobacco Extract on TLR4 Inflammatory Pathway in Periodontal Tissues of Mice with Periodontitis

WU Yunfei1, ZHANG Li2, ZHANG Xufeng2, FU Qiya2*   

  1. 1. Department of Stomatology, the First Affiliated Hospital of Hainan Medical University, Haikou 570102, China;
    2. School of Stomatology, Hainan Medical University, Haikou 570102, China.
  • Received:2018-10-28 Online:2019-07-25 Published:2019-07-24

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摘要： 目的:探究烟草提取物对牙周炎小鼠牙周组织中Toll样受体4(TLR4)炎症通路的影响。方法:将小鼠随机分为对照租、模型组、烟草提取物低、中、高剂量组。Micro-CT扫描观察牙周骨骼变化;酶联免疫法(Elisa)检测血清中肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)水平;HE染色检测牙周组织病理形态学变化;实时荧光定量PCR(qRT-PCR)、免疫印迹(WB)、免疫组化法检测牙周组织中TLR4、肿瘤坏死因子受体相关因子6(TRAF6)、核因子κB(NF-κB)表达。结果:模型组骨体积比、骨表面积比、骨小梁厚度均显著降低,骨小梁间隙增大,血清中TNF-α、IL-1β、IL-6水平、牙周组织中TLR4、TRAF6、NF-κB 表达均显著升高(P＜0.05)。与模型组相比,烟草提取物各组骨体积比、骨表面积比、骨小梁厚度降低,骨小梁间隙增大,血清TNF-α、IL-1β、IL-6水平、牙周组织中TLR4、TRAF6、NF-κB 表达均显著升高(P＜0.05)。结论:烟草提取物可加重牙周炎小鼠牙周组织炎症反应,其机制可能与上调牙周组织中TLR4信号通路有关

关键词: 牙周炎, 烟草提取物, Toll样受体4, 牙周组织, 机制研究

Abstract: Objective: To explore the effects of tobacco extract on Toll-like receptor 4 (TLR4) inflammatory pathway in periodontal tissues of mice with periodontitis. Methods: Rats were randomly divided into control group, model group, low, medium, and high dose groups. Micro-CT scan was used to observe periodontal bone changes. The levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in serum were detected by enzyme-linked immunoassay (Elisa). HE staining was used to detect the pathomorphological changes of periodontal tissues. The expressions of TLR4, tumor necrosis factor receptor related factor 6 (TRAF6), and nuclear factor κB (NF-κB) in periodontal tissues were detected by real-time fluorescence quantitative PCR (qRT-PCR), immunoblotting (WB), and immunohistochemistry. Results: Bone volume ratio, bone surface area ratio, and trabecular thickness in model group were significantly decreased, trabecular space was increased, serum levels of TNF-α, IL-1β, IL-6, expression of TLR4, TRAF6, and NF-κB in periodontal tissue were significantly increased (P<0.05). Compared with the model group, the bone volume ratio, bone surface area ratio, trabecular thickness, trabecular space, serum levels of TNF-α, IL-1β, IL-6, expression of TLR4, TRAF6 and NF-κB in periodontal tissues of the tobacco extract groups were significantly increased (P<0.05). Conclusion: Tobacco extract can aggravate periodontal tissue inflammatory reaction in mice with periodontitis, which may be related to up-regulation of TLR4 signaling pathway in periodontal tissue.

Key words: periodontitis , tobacco extract, toll like receptor 4, periodontal tissue, mechanism research