Mongersen对大鼠牙周炎抗炎效果观察

doi:10.13701/j.cnki.kqyxyj.2020.04.018

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (4): 382-386.DOI: 10.13701/j.cnki.kqyxyj.2020.04.018

• 牙周病学与黏膜病学研究 • 上一篇下一篇

Mongersen对大鼠牙周炎抗炎效果观察

丁小函¹, 岳轶云¹, 夏博园¹, 李艳¹, 刘歆婵², 武洲³, 于维先^4*

1.吉林大学口腔医院牙周病科吉林长春 130021;
2.吉林大学口腔医学院老年口腔科吉林长春 130021;
3.日本九州大学齿学研究院日本福冈 821-8581;
4.吉林省牙发育及颌骨重塑与再生重点实验室吉林长春 130021

收稿日期:2019-07-22 出版日期:2020-05-28 发布日期:2020-05-28
通讯作者: 于维先,E-mail:yu-wei-xian@163.com
作者简介:丁小函 (1993~ ) ,女,河北人,硕士,医师,主要从事牙周免疫炎症的研究。
基金资助:
吉林省科技厅国际合作项目(编号:20180414053GH); 吉林省科技厅自然科学基金项目(编号:20190201058JC)

Observation of Mongersen's Anti-inflammatory Effect on Periodontitis Rat

DING Xiaohan¹, YUE Yiyun¹, XIA Boyuan¹, LI Yan¹, LIU Xinchan², WU Zhou³, YU Weixian^4*

1. Department of Periodontics, School of Stomatology, Jilin University, Changchun 130021, China;
2. Department of Dental Implantoloty, School of Stomatology, Jilin University, Changchun 130021, China;
3. Institute of Dentistry, University of Kyushu, Fukuoka-ken 821-8581, Japan;
4. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, School of Stomatology, Jilin University, Changchun 130021, China

Received:2019-07-22 Online:2020-05-28 Published:2020-05-28

摘要/Abstract

摘要： 目的: 在体内观察Mongersen对大鼠牙周炎的抗炎效果。方法: 选取健康8周龄雄性Wistar大鼠22只,随机选取6只为空白对照(C0组),剩余16只构建大鼠牙周炎模型,之后将其随机分3组,其中1组为牙周炎对照组(C组),另外2组分别注射Mongersen(M组)以及生理盐水(N组),2周后将其全部处死,检测其临床指标及病理学改变,并采用qRT-PCR检测牙龈组织炎症因子的表达。结果: 临床指标及组织学观察结果显示M组相比C组及N组炎症缓解;qRT-PCR结果显示M组与N组及C组相比TNF-α、IL-1β等炎症因子基因的表达显著降低,差异有统计学意义(P＜0.001)。结论: Mongersen可明显降低大鼠牙周组织炎症程度及其炎症因子的表达。

关键词: Mongersen, 牙周炎, 免疫

Abstract: Objective: To investigate the anti-inflammatory effect of mongersen on periodontitis in rat. Methods: A total of 22 healthy male Wistar rats aged 8 weeks were randomly selected. The models of periodontitis were established in 16 rats which were randomly divided into 3 groups, group M (mongersen), group N (saline), and group C (negative controls), the remaining 6 rats were Group C0 (no intervention). All drugs were injected into the rat's maxillary buccal and palatine side of gingival via a microsyringe. Two weeks later, clinical parameters and morphological analysis were measured, then all rats were sacrificed, and the histological changes of periodontal tissues in each group were observed by HE stain and the expression of inflammatory cytokines in gingival tissues of rats were detected by qRT-PCR. Results: Clinical indicators and histological observation showed that in Group M the inflammation was alleviated compared with the group N and C. The qRT-PCR results showed that the gene expressions of TNF-α, IL-1β, IL-6, and INOS in the group M were significantly decreased compared with those of group N and C (P<0.001). Conclusion: Mongersen has anti-inflammatory effect on periodontitis rat.

Key words: Mongersen, periodontitis, immunity

丁小函, 岳轶云, 夏博园, 李艳, 刘歆婵, 武洲, 于维先. Mongersen对大鼠牙周炎抗炎效果观察[J]. 口腔医学研究, 2020, 36(4): 382-386.

DING Xiaohan, YUE Yiyun, XIA Boyuan, LI Yan, LIU Xinchan, WU Zhou, YU Weixian. Observation of Mongersen's Anti-inflammatory Effect on Periodontitis Rat[J]. Journal of Oral Science Research, 2020, 36(4): 382-386.

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Mongersen对大鼠牙周炎抗炎效果观察

Observation of Mongersen's Anti-inflammatory Effect on Periodontitis Rat

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