牙周炎宿主转化生长因子-β+调节性B细胞变化特点

doi:10.13701/j.cnki.kqyxyj.2020.02.011

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (2): 135-138.DOI: 10.13701/j.cnki.kqyxyj.2020.02.011

牙周炎宿主转化生长因子-β⁺调节性B细胞变化特点

韩亚琨^*, 于程程, 于艳

吉林医药学院附属医院口腔科吉林吉林 132013

收稿日期:2019-01-15 出版日期:2020-02-28 发布日期:2020-04-24
通讯作者: 韩亚琨,E-mail:hanyk1986@qq.com
作者简介:韩亚琨(1986~ ),男,黑龙江鸡东人,博士,副主任医师,主要从事牙周病学研究。
基金资助:
吉林省教育厅十三五科学研究规划项目(编号:JJKH20200463KJ);吉林省中医药科技计划(编号:2018127)

Change of TGF-β⁺ Regulatory B Cells during Periodontitis

HAN Yakun^*, YU Chengcheng, YU Yan

Department of Stomatology, Affiliated Hospital of Jilin Medical College, Jilin 132013, China

Received:2019-01-15 Online:2020-02-28 Published:2020-04-24

摘要/Abstract

摘要： 目的:探讨牙周炎时宿主不同组织内转化生长因子β(transforming growth factor-β,TGF-β)⁺调节性B细胞的变化特点,比较健康个体与牙周炎模型之间的差异,分析其潜在的作用机制,为进一步揭示牙周炎病理机制提供理论支持。方法:采用SD大鼠建立实验性牙周炎病理模型。采用流式细胞术分析各组不同组织内TGF-β⁺调节性B细胞占总淋巴细胞的比例,采用实时荧光定量聚合酶链反应(Real-time polymerase chain reaction,Real-time PCR)检测各样本中B细胞发育相关因子B细胞活化因子(B-cellactivatingfactor, BAFF)、增殖诱导配体(aproliferation-inducingligand, APRIL)及白细胞介素-33(interleukin-33,IL-33)的表达水平。结果:与健康组比较,牙周炎大鼠牙龈中TGF-β⁺调节性B细胞比例显著上调(P<0.05);而在外周血中两者比较差异无统计学意义(P>0.05)。在牙龈中,牙周炎大鼠各检测基因的相对表达水平与健康组比较差异显著(P<0.05);在外周血中,牙周炎大鼠与健康组各检测基因的相对表达水平比较差异无统计学意义(P>0.05)。结论:牙周炎时宿主牙龈中TGF-β⁺调节性B细胞上调,这与局部BAFF、APRIL及IL-33等细胞因子表达水平呈正相关。

关键词: 牙周炎, 调节性B细胞, 宿主免疫, 白细胞介素33, 转化生长因子β

Abstract: Objective: To explore the change of TGF-β⁺ regulatory B cells during periodontitis, and to find out its possible mechanism. Methods: Experimental periodontitis animal model using SD rats was set. The animals were sacrificed after 28 days. The gingiva and peripheral blood were obtained for further research. The proportion of TGF-β⁺ regulatory B cells was detected by flow cytometry. The expression of BAFF, APRIL, and IL-33 were analyzed by real time PCR. Results: In comparison with healthy animals, the proportion of TGF-β⁺ regulatory B cells in periodontitis ones were significantly higher (P<0.05). However, in the peripheral blood, they were similar (P>0.05). Compared with healthy ones, the expression of BAFF, APRIL, and IL-33 in the gingiva were significantly higher in the periodontitis group. However, in the peripheral blood, there was no significant difference. Conclusion: The higher proportion of TGF-β⁺ regulatory B cells detected in gingiva of periodontitis animals may relate to the increased expression of local BAFF, APRIL, and IL-33.

Key words: periodontitis, regulatory B cells, host immunity, interleukin-33, transforming growth factor-β

韩亚琨, 于程程, 于艳. 牙周炎宿主转化生长因子-β⁺调节性B细胞变化特点[J]. 口腔医学研究, 2020, 36(2): 135-138.

HAN Yakun, YU Chengcheng, YU Yan. Change of TGF-β⁺ Regulatory B Cells during Periodontitis[J]. Journal of Oral Science Research, 2020, 36(2): 135-138.

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牙周炎宿主转化生长因子-β⁺调节性B细胞变化特点

Change of TGF-β⁺ Regulatory B Cells during Periodontitis

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牙周炎宿主转化生长因子-β+调节性B细胞变化特点

Change of TGF-β+ Regulatory B Cells during Periodontitis

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牙周炎宿主转化生长因子-β⁺调节性B细胞变化特点

Change of TGF-β⁺ Regulatory B Cells during Periodontitis