狗肝菜多糖对辐射损伤后大鼠腮腺中血管内皮生长因子的表达变化和意义

doi:10.13701/j.cnki.kqyxyj.2019.02.006

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (2): 125-128.DOI: 10.13701/j.cnki.kqyxyj.2019.02.006

狗肝菜多糖对辐射损伤后大鼠腮腺中血管内皮生长因子的表达变化和意义

覃珊珊^1,2,3,4,王代友^1*,眭斌¹,张岩¹,孙喆¹

1. 广西医科大学附属口腔医院广西南宁 530021;
2. 广西口腔颌面修复与重建研究自治区级重点实验室广西南宁 530021;
3. 广西颅颌面畸形临床医学研究中心广西南宁 530021;
4. 广西颌面外科疾病诊治研究重点实验室广西南宁 530021

收稿日期:2018-07-12 出版日期:2019-02-18 发布日期:2019-02-25
通讯作者: *通讯作者王代友,E-mail:wangdaiyou@hotmail.com
作者简介:覃珊珊(1992~ ),女,广西宾阳人,硕士在读,研究方向为头颈部肿瘤放射损伤。
基金资助:
国家自然科学基金(编号:8156030117)

Effect of Dicliptera Chinensis Polysaccharide on Expression and Significance of VEGF in Rat Parotid Gland after Radiation Injury.

QIN Shan-shan^1,2,3,4, WANG Dai-you^1*,SUI Bin¹, ZHANG Yan¹,SUN Zhe¹

1. Guangxi Medical University of Stomatology, Nanning 530021, China;
2. Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Nanning 530021, China;
3. Guangxi Clinical Medical Research Center for craniofacial Deformity, Nanning 530021, China;
4. Guangxi Key Laboratory of Oral and Maxillofacial Surgery Diseases Treatment, Nanning 530021, China.

Received:2018-07-12 Online:2019-02-18 Published:2019-02-25

摘要/Abstract

摘要： 目的: 建立大鼠辐射损伤修复模型,探讨狗肝菜多糖(dicliptera chinensis polysaccharide,DCP)对辐射损伤后大鼠腮腺中血管内皮生长因子 (vascular endothelial growth factor ,VEGF)基因的调节及其意义。方法: 成年无特定病原体 (specific pathogen free,SPF )级健康雄性SD大鼠(200~220 g)96只,采用随机数字表法分为正常组(未照射+0.9％氯化钠溶液)、放射组(18 Gy ⁶⁰Co γ射线照射+0.9％氯化钠溶液)、正常给药组(未照射+200 mg/kg DCP溶液)和放射给药组(18 Gy ⁶⁰Co γ射线照射+200 mg/kg DCP溶液),每组24只。每周记录体重,监测大鼠的一般情况,苏木精-伊红(hematoxylin-eosin staining,HE)染色观察大鼠腮腺组织形态学变化,实时荧光定量聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction,FQ-PCR)和Western blot检测VEGF的表达。结果: 放射组和放射给药组的大鼠体重减轻;HE染色结果显示放射组大鼠腮腺细胞和血管损伤较重,放射给药组大鼠腮腺细胞和血管损伤较轻;FQ-PCR 检测结果显示,放射组大鼠VEGF mRNA相对表达量低于其他组(P＜0.05);Western blot检测结果显示,放射组大鼠VEGF蛋白表达低于其他组(P＜0.05)。结论: DCP可以减轻腮腺损伤、上调VEGF表达;DCP可能对大鼠腮腺辐射性损伤有修复作用。

关键词: 血管内皮生长因子, 放射性损伤, 狗肝菜多糖, 腮腺, 大鼠

Abstract: Objective : To explore the regulation and significance of Dicliptera Chinensis Polysaccharide(DCP) on VEGF in rat parotid gland after radiation injury. Methods: Ninty-six adult SPF healthy male SD rats (200g-220g) were randomly divided into normal group (non-irradiated with 0.9% sodium chloride solution), radiation group (18Gy ⁶⁰Co γ-ray irradiation with 0.9% sodium chloride solution), normal treatment group (non-irradiated with 200 mg/kg Dicliptera Chinensis Polysaccharide solution), and radiotherapy group (18Gy ⁶⁰Co γ-ray irradiation with 200 mg/kg Dicliptera Chinensis Polysaccharide solution). Each group had 24 rats. All rats were weighed once a week to monitor the general condition. Hematoxylin-eosin staining (HE) was used to observe the morphological changes of the parotid gland in rats. The expression of VEGF was detected by real-time fluorescent quantitative polymerase chain reaction,FQ-PCR and Western blotting. Results: The weight loss of rats was observed in the radia tion group and the radiotherapy group. HE staining results showed that the salivary gland cells and blood vessels in the radiation group were damaged heavier. And the radiotherapy group had less damage. The relative expression of VEGF gene detected by FQ-PCR in radiation group was lower than other groups (P＜0.05). The expression of VEGF protein detected by WB in the radioactive group was lower than other groups (P＜0.05). Conclusion: DCP can alleviate parotid damage and up-regulate VEGF expression. DCP may repair the radial damage of rat parotid gland.

Key words: VEGF Radiation damage, Dicliptera chinensis polysaccharide, Parotid gland, Rat

覃珊珊,王代友,眭斌,张岩,孙喆. 狗肝菜多糖对辐射损伤后大鼠腮腺中血管内皮生长因子的表达变化和意义[J]. 口腔医学研究, 2019, 35(2): 125-128.

QIN Shan-shan, WANG Dai-you,SUI Bin, ZHANG Yan,SUN Zhe.. Effect of Dicliptera Chinensis Polysaccharide on Expression and Significance of VEGF in Rat Parotid Gland after Radiation Injury.[J]. Journal of Oral Science Research, 2019, 35(2): 125-128.

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狗肝菜多糖对辐射损伤后大鼠腮腺中血管内皮生长因子的表达变化和意义

Effect of Dicliptera Chinensis Polysaccharide on Expression and Significance of VEGF in Rat Parotid Gland after Radiation Injury.

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