口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (5): 554-557.DOI: 10.13701/j.cnki.kqyxyj.2018.05.022

• 口腔肿瘤学研究 • 上一篇    下一篇

槲皮素对ACC-M细胞裸鼠移植瘤抑瘤效果的体内实验研究

侯国玲1, 姚瑶2, 万光勇2*   

  1. 1. 潍坊医学院口腔医学院 山东 潍坊 261000;
    2. 泰安市中心医院口腔科 山东 泰安 271000
  • 收稿日期:2017-11-15 出版日期:2018-05-28 发布日期:2018-05-29
  • 通讯作者: 万光勇, E-mail: wgykq@163.com
  • 作者简介:侯国玲(1990~ ),女,山东泰安人,硕士在读,主要从事口腔颌面部肿瘤研究。
  • 基金资助:
    山东省泰安科技发展计划(编号:2015NS2099)

Inhibitory Effect of Quercetin on ACC-M Cell Xenografts in Nude Mice in Vivo.

HOU Guo-ling1, YAO Yao2, WAN Guang-yong2*   

  1. 1. Department of Stomatology, Weifang Medical University, Weifang 261000, China;
    2. Department of Stomatology, Tai'an City Central Hospital, Tai'an 271000, China
  • Received:2017-11-15 Online:2018-05-28 Published:2018-05-29

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摘要： 目的:观察槲皮素对ACC-M细胞裸鼠移植瘤的抑制作用,探讨其作用机制。方法:常规将ACC-M 细胞接种于裸鼠右侧背部皮下,待肿瘤长至直径约6mm时随机分为5组,即空白对照组、槲皮素低、中、高剂量组、紫杉醇组,每组6只。定期测量肿瘤体积,绘制肿瘤生长曲线。给药3周后处死裸鼠,剥取肿瘤,称瘤重,计算抑瘤率。苏木精-伊红(hematoxylin-eosin,HE)染色,免疫组织化学法检测血管内皮生长因子(vascular endothelial growth factor,VEGF)和增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)的表达情况。结果:给药3周后,各组的抑瘤率分别为11.45%、37.67%、42.49%、43.46%。VEGF表达的平均吸光度值分别为(0.420±0.039)、(0.367±0.040)、(0.315±0.033)、(0.270±0.016)、(0.298±0.044),各组比较差异有统计学意义(P<0.05);PCNA阳性表达率分别为(68.45±3.05)%、(58.84±3.33)%、(49.83±1.81)%、(44.90±1.85)%、(41.23±1.04)%,各组比较差异有统计学意义(P<0.01)。结论:槲皮素可抑制 ACC-M 细胞在裸鼠体内增殖,其机制可能通过抑制 VEGF和PCNA的表达发挥作用。

关键词: 槲皮素, 腺样囊性癌, 血管内皮生长因子, 增殖细胞核抗原

Abstract: Objective: To investigate the inhibitory effects of quercetin on the salivary adenoid cystic carcinoma cancer ACC-M cell in nude mice, and to explore its possible mechanism. Methods: ACC-M cells were inoculated subcutaneously on the right side of nude mice.The mice with tumor were randomly divided into blank control group, quercetin groups with low, medium, and high dose, and paclitaxel group. When the tumor diameter reached 6 mm, the tumor volume was regularly measured and the tumor growth curve was plotted. After 3 weeks of treatment, nude mice were sacrificed, the tumor was removed and weighed, and the tumor inhibition rate was calculated. HE staining and immunohistochemistry were used to detect the expression of VEGF and PCNA in tumor tissues. Results: After 3 weeks of treatment, the inhibition rate of each group was 11.45%, 37.67%, 42.49%, and 43.46%, respectively. The mean density of VEGF protein was (0.420±0.039), (0.367±0.040), (0.315±0.033), (0.270±0.016), and (0.298±0.044). Compared with the blank control group, the groups of quercetin was statistically significant (P<0.05). And the positive expression rate of PCNA protein was (68.45±3.05)%, (58.84±3.33)%, (49.83±1.81)%, (44.90±1.85)%, and (41.23±1.04)% (P<0.01). Conclusion: Quercetin can inhibit the proliferation of ACC-M cells in nude mice. The mechanism may be the inhibition of VEGF and PCNA expression.

Key words: Quercetin, Adenoid cystic carcinoma, Vascular endothelial growth factor, Proliferating cell nuclear antigen