口腔鳞状细胞癌患者外周血淋巴细胞免疫分型及临床意义

doi:10.13701/j.cnki.kqyxyj.2018.05.021

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (5): 548-553.DOI: 10.13701/j.cnki.kqyxyj.2018.05.021

口腔鳞状细胞癌患者外周血淋巴细胞免疫分型及临床意义

杨柳¹, 陈娟¹, 单忠艳¹, 雷焯¹, 全宏志², 唐瞻贵^2*

1. 中南大学湘雅口腔医学院湖南长沙 410008;
2. 中南大学湘雅口腔医院口腔颌面外科湖南长沙 410008

收稿日期:2018-03-16 出版日期:2018-05-28 发布日期:2018-05-29
通讯作者: 唐瞻贵,E-mail:tangzhangui@aliyun.com
作者简介:杨柳(1991~ ),女,湖南人,硕士在读,主要从事口腔颌面外科学研究工作。
基金资助:
国家自然科学基金面上项目(编号:81671003)
中国博士后第59批面上项目(编号:2016M592454)
湖南省自然科学基金青年基金(编号:2016JJ4093)

Immunophontyping of Peripheral Blood Lymphocytes in Patients with Oral Squamous Cell Carcinoma and Its Clinical Significance.

YANG Liu¹, CHEN Juan¹, SHAN Zhong-yan¹, LEI Zhuo¹, QUAN Hong-zhi², TANG Zhan-gui^2*

1. Xiangya School of Stomatology,Central South University,Changsha 410008,China;
2.Department of Oral and Maxillofacial Surgery,Xiangya Stomatological Hospital of Central South University,Changsha 410008,China

Received:2018-03-16 Online:2018-05-28 Published:2018-05-29

摘要/Abstract

摘要： 目的:研究口腔鳞状细胞癌(Oral squamous cell carcinoma,OSCC)患者外周血中不同淋巴细胞免疫表型的分布特征,探讨其与癌变相关危险因素及肿瘤生物学行为之间的关系。方法:选取49例口腔鳞状细胞癌患者和15例正常成人的外周血,应用流式细胞术检测外周血中T、B淋巴细胞及其各免疫表型的分布特征,并对其与槟榔咀嚼、吸烟、饮酒等癌变危险因素及肿瘤TNM分期和淋巴结转移相关性进行分析。结果:OSCC患者外周血中CD8⁺T细胞、效应记忆T细胞(CD4⁺Teff、 CD8⁺Teff)、CD4⁺PD1⁺T、CD8⁺PD1⁺T、CD8⁺PD1⁺Teff、CD8⁺Tim3+T、CD8⁺Tim3⁺Teff的表达均显著高于正常对照组,而CD4⁺初始T细胞(Tn)、B细胞则显著降低,且CD4⁺Tn/CD4⁺Teff、CD8⁺Tn/CD8⁺Teff比值均明显下降(P<0.05);OSCC患者外周血中CD8⁺T细胞、CD4⁺Teff、CD8⁺Teff细胞的增高与淋巴结转移及肿瘤分期显著相关,且伴淋巴结转移或中晚期患者外周血中CD4⁺Tn细胞及CD4/CD8、CD4⁺Tn/Teff的比例均显著降低;OSCC患者外周血中CD4⁺Teff比例的降低和CD4⁺Tn细胞比例的升高与槟榔咀嚼显著相关(P<0.05),而饮酒和吸烟与T、B细胞各免疫亚型的改变无显著相关。结论:本研究发现OSCC患者外周血中淋巴细胞各免疫表型发生了改变,且这种改变与槟榔咀嚼及肿瘤进展显著相关。

关键词: 口腔鳞状细胞癌, 外周血淋巴细胞, 淋巴结转移, TNM分期, 癌变危险因素

Abstract: Objective: To investigate the distribution of immunophenotypes of peripheral blood lymphocytes in patients with oral squamous cell carcinoma (OSCC) and to explore its role in the tumor progression and its relationship with cancer-associated risk factors. Methods: Forty-nine patients with OSCC and 15 normal adults were enrolled in this study. Peripheral T and B lymphocytes and their immunophenotype distributions were detected by flow cytometry. The relevance of this distribution was analyzed with cancer-associated risk factors, including betel-chewing, smoking, alcohol drinking, tumor TNM stage, and lymph node metastasis. Results: The frequency of CD8⁺ T cells, effector memory T cells (CD4⁺Teff CD8⁺Teff), CD4⁺PD1⁺T, CD8⁺PD1⁺T, CD8⁺PD1⁺Teff, CD8⁺Tim3⁺T, and CD8⁺Tim3⁺Teff in OSCC was significantly higher than that of normal control group, whereas the CD4⁺ Naive T cells (Tn) and B cells were significantly decreased, and CD4⁺Tn/CD4⁺ Teff and CD8⁺Tn/CD8⁺Teff ratios were significantly decreased. The decrease of CD4⁺ Teff frequency was significantly associated with betel nut chewing, but the expression of CD4⁺ Tn cells was significantly increased (P<0.05). The history of drinking and smoking did not show any significantly difference in T and B cell immune subtypes. Conclusion: In this study, there were significant changes in the immunophenotypes of peripheral blood lymphocytes in patients with OSCC. The changes were significantly associated with betel chewing and tumor progression.

Key words: Oral squamous cell carcinoma, Peripheral blood lymphocytes, Lymph node metastasis, Tumor progression, Cancer-associated risk factors

杨柳, 陈娟, 单忠艳, 雷焯, 全宏志, 唐瞻贵. 口腔鳞状细胞癌患者外周血淋巴细胞免疫分型及临床意义[J]. 口腔医学研究, 2018, 34(5): 548-553.

YANG Liu, CHEN Juan, SHAN Zhong-yan, LEI Zhuo, QUAN Hong-zhi, TANG Zhan-gui. Immunophontyping of Peripheral Blood Lymphocytes in Patients with Oral Squamous Cell Carcinoma and Its Clinical Significance.[J]. Journal of Oral Science Research, 2018, 34(5): 548-553.

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口腔鳞状细胞癌患者外周血淋巴细胞免疫分型及临床意义

Immunophontyping of Peripheral Blood Lymphocytes in Patients with Oral Squamous Cell Carcinoma and Its Clinical Significance.

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