口腔医学研究

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (5): 428-432.DOI: 10.13701/j.cnki.kqyxyj.2020.05.007

• 口腔肿瘤学研究 • 上一篇    下一篇

口腔鳞状细胞癌患者外周血microRNA的表达及预后意义

杨凯成1, 杨蕾1, 赵建广1, 罗风玉2, 陈彦平1*, 崔子峰1, 陈赫1, 满莎莎3   

  1. 1.河北医科大学第四医院口腔科 河北 石家庄 050011;
    2.灵寿县人民医院口腔科 河北 石家庄 050500;
    3.河北医科大学第三医院口腔科 河北 石家庄 050051
  • 收稿日期:2019-08-02 出版日期:2020-06-16 发布日期:2020-06-18
  • 通讯作者: * 陈彦平,E-mail: cyp1962@126.com
  • 作者简介:杨凯成(1985~),男,河北石家庄人,主治医师,研究方向:口腔肿瘤。
  • 基金资助:
    河北省卫生厅青年科技课题(编号:20190728)

Expression and Prognostic Significance of microRNAs in Peripheral Blood of Patients with Oral Squamous Cell Carcinoma

YANG Kaicheng1, YANG Lei1, ZHAO Jianguang1, LUO Fengyu2, CHEN Yanping1*, CUI Zifeng1, CHEN He1, MAN Shasha3   

  1. 1. Department of Stomatology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China;
    2. Department of Stomatology, People's Hospital of Lingshou County, Shijiazhuang 050500, China;
    3. Department of Stomatology, Third Hospital of Hebei Medical University, Shijiazhuang 050051, China
  • Received:2019-08-02 Online:2020-06-16 Published:2020-06-18

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摘要： 目的:筛选口腔鳞状细胞癌(OSCC)患者外周血差异表达的microRNA(miRNA),探讨其与临床参数及其预后意义。方法:利用qRT-PCR技术检测OSCC外周血中miRNA的变化,扩大样本量进行验证,并分析miRNA与OSCC临床病理指标和预后的关系。结果:总共检测84种miRNA,其中OSCC患者外周血中上调大于2倍的miRNA有8个;下调小于0.5倍的有18个。其中miR-222-3p和miR-423-5p在外周血的表达与pTNM分期和淋巴结转移显著相关(P＜0.05)。miR-222-3p高表达和miR-423-5p低表达的患者生存期均较短(P＜0.05)。多因素分析结果提示miR-222-3p高表达可作为OSCC患者生存的独立预后因素。结论:miR-222-3p和miR-423-5p在OSCC患者外周血中存在差异表达,并有望成为OSCC早期诊断和预测预后的生物标志物。

关键词: 口腔鳞状细胞癌, miR-222-3p, miR-423-5p, 生物标志物, 预后

Abstract: Objective: To screen differentially expressed microRNAs (miRNAs) in peripheral blood of patients with oral squamous cell carcinoma (OSCC) by qRT-PCR, and further to explore the relationship between differentially expressed miRNAs and clinical parameters and prognosis. Methods: The changes of miRNA in peripheral blood of patients with OSCC were observed by qRT-PCR, and the relationship between miRNA and clinicopathological parameters and prognosis was analyzed. Results: A total of 84 kinds of miRNAs were detected, of which 8 were up-regulated more than twice in peripheral blood of patients with OSCC and 18 were down-regulated less than 0.5 times. The expression of miR-222-3p and miR-423-5p in peripheral blood was significantly correlated with pTNM stage and lymph node metastasis (P＜0.05). The patients with high expression of miR-222-3p and low expression of miR-423-5p had a worse prognosis (P＜0.05). Multivariate analysis showed that the overexpression of miR-222-3p was an independent prognostic factor for the OSCC patients. Conclusions: miR-222-3p and miR-423-5p were abnormally expressed in the peripheral blood in OSCC patients, and they were expected to be biomarkers for early diagnosis and predicting prognosis of OSCC.

Key words: OSCC, miR-222-3p, miR-423-5p, biomarkers, prognosis