同源形成素样蛋白2基因对口腔鳞状细胞癌侵袭、迁移及核因子-κB信号的影响

doi:10.13701/j.cnki.kqyxyj.2019.02.009

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (2): 137-141.DOI: 10.13701/j.cnki.kqyxyj.2019.02.009

同源形成素样蛋白2基因对口腔鳞状细胞癌侵袭、迁移及核因子-κB信号的影响

李勇伟^*,邱勋定,顾明,邓旎,谢莉莉

海南省人民医院口腔科海南海口 570311

收稿日期:2018-09-04 出版日期:2019-02-18 发布日期:2019-02-25
通讯作者: 李勇伟,E-mail:87751194@qq.com
作者简介:李勇伟(1973~ ),男,广东吴川人,学士,主管技师,研究方向为口腔修复医学及技术。
基金资助:
2018年海南省卫生计生行业科研项目(编号:18A200009)

Effect of FMNL2 Gene on Invasion, Migration, and NF-κB Signal in Oral Squamous Cell Carcinoma.

LI Yong-wei^*, QIU Xun-ding, GU Ming, DENG Yi, XIE Li-li

Department of Stomatology, Hainan Provincial People's Hospital, Haikou 570311, China.

Received:2018-09-04 Online:2019-02-18 Published:2019-02-25

摘要/Abstract

摘要： 目的: 探讨下调同源形成素样蛋白2(formin-like 2,FMNL2)基因表达对口腔鳞状细胞癌侵袭、迁移及核因子-κB(nuclear factor-κB,NF-κB)信号的影响。方法: FMNL2的特异性小干扰RNA(small interfering RNA,siRNA)(si-FMNL2组)及阴性对照siRNA(NC组)转染人口腔鳞状细胞癌CAL-27细胞,二硫代氨基甲酸吡咯烷 (pyrro1idinedithiocarbamate,PDTC)作为NF-κB 信号抑制剂,处理48 h,Western blot检测FMNL2、E-钙粘蛋白(E-cadherin)、波形蛋白(Vimentin)、NF-κB p65和IκBα蛋白表达;Transwell小室检测细胞侵袭和迁移能力。结果: 与NC组比较,si-FMNL2组和PDTC组细胞侵袭和迁移能力均明显降低,E-cadherin蛋白表达明显升高,Vimentin、NF-κB p65和IκBα蛋白表达明显降低(P<0.05)。与PDTC组比较,PDTC+si-FMNL2组细胞侵袭和迁移能力均明显降低,E-cadherin蛋白表达明显升高,Vimentin蛋白表达明显降低(P<0.05)。结论: 下调FMNL2基因表达可通过抑制NF-κB信号通路逆转上皮-间充质转化(epithelial-mesenchymal transition,EMT),从而抑制口腔鳞状细胞癌侵袭和迁移能力。

关键词: 口腔鳞状细胞癌, 同源形成素样蛋白2基因, 侵袭, 迁移, 上皮-间充质转化, 核因子-κB信号

Abstract: Objective : To investigate the effect of down-regulation of FMNL2 gene expression on invasion, migration, and NF-κB signal in oral squamous cell carcinoma (OSCC). Methods: FMNL2 specific siRNA (si-FMNL2) and negative control siRNA (NC) were transfected into human oral squamous cell carcinoma cells CAL-27. PDTC was set as NF-κB signal inhibitor. After the cells were treated for 48h, western blotting was used to detect FMNL2, E-cadherin, Vimentin, NF-κBp65, and IκBα protein expression. Results: Compared with the blank group, the cell invasion and migration ability in si-FMNL2 group and PDTC group decreased obviously, the expression of E-cadherin protein increased obviously, and the expression of Vimentin, NF-κBp65, and IκBα protein decreased significantly (P<0.05). Compared with the PDTC group, the cell invasion and migration ability in PDTC+si-FMNL2 group decreased obviously, the expression of E-cadherin protein increased obviously, and the expression of Vimentin protein decreased significantly (P<0.05). Conclusion: Downregulation of FMNL2 gene expression can reverse EMT by inhibiting NF-κB signaling pathway, thus inhibiting the invasion and migration of oral squamous cell carcinoma.

Key words: Oral squamous cell carcinoma, FMNL2 gene, Invasion, Migration, EMT, NF-κB signal

李勇伟,邱勋定,顾明,邓旎,谢莉莉. 同源形成素样蛋白2基因对口腔鳞状细胞癌侵袭、迁移及核因子-κB信号的影响[J]. 口腔医学研究, 2019, 35(2): 137-141.

LI Yong-wei, QIU Xun-ding, GU Ming, DENG Yi, XIE Li-li. Effect of FMNL2 Gene on Invasion, Migration, and NF-κB Signal in Oral Squamous Cell Carcinoma.[J]. Journal of Oral Science Research, 2019, 35(2): 137-141.

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同源形成素样蛋白2基因对口腔鳞状细胞癌侵袭、迁移及核因子-κB信号的影响

Effect of FMNL2 Gene on Invasion, Migration, and NF-κB Signal in Oral Squamous Cell Carcinoma.

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