槲皮素对人口腔上皮细胞再生的生物学效应研究

doi:10.13701/j.cnki.kqyxyj.2018.09.017

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (9): 991-994.DOI: 10.13701/j.cnki.kqyxyj.2018.09.017

槲皮素对人口腔上皮细胞再生的生物学效应研究

米娅莎尔·呼加合买提,李雪,吕慧欣,丁鑫鑫,周延民^*

吉林大学口腔医院种植中心吉林长春 130021

收稿日期:2018-03-16 出版日期:2018-09-28 发布日期:2018-09-25
通讯作者: 周延民,E-mail:zhouym62@126.com
作者简介:米娅莎尔·呼加合买提(1987~ ),女,新疆人,硕士,主治医师,主要从事种植体周围软硬组织再生相关研究。

Effects of Quercetin on Human Oral Keratinocyte Regeneration.

Miyashaer·Hujiahemaiti, LI Xue, LV Hui-xin, DING Xin-xin, ZHOU Yan-min^*.

Department of Dental Implantology, School and Hospital of Stomatology, Jilin University, Changchun 130021,China.

Received:2018-03-16 Online:2018-09-28 Published:2018-09-25

摘要/Abstract

摘要： 目的: 研究槲皮素对人口腔角质细胞(human oral keratinocytes,HOKs)再生的生物学效应。方法: 通过细胞计数试剂盒-8(cell counting kit-8,CCK-8)及迁移实验验证槲皮素对人牙龈角质细胞的增殖与迁移的药理作用。通过牙龈卟啉单胞菌脂多糖作为炎症刺激因子构建体外炎症模型,实时荧光定量聚合酶链反应(real time quantitative polymerase chain reaction,RT-qPCR)法检测转化生长因子(transforming growth factor,TGF)-β1和TGF-β3的表达及炎症因子白细胞介素(interleukin,IL)-1β和肿瘤坏死因子(tumor necrosis factor,TNF)-α的基因表达验证槲皮素的抗炎效果。酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测TGF-β1和TGF-β3的分泌水平。结果: 20 μmol/L槲皮素促进HOKs的增殖与迁移,并提高TGF-β3的mRNA水平与蛋白质表达,而50 μmol/L槲皮素对TGF-β1的表达有促进作用。20、50 μmol/L的槲皮素都有明显的抗炎作用。结论: 20 μmol/L槲皮素在体外实验中能够促进创面愈合并且促进HOKs再生。

关键词: 槲皮素, 角化龈, 牙龈角质细胞, 牙周软组织再生

Abstract: Objective: To assess the potential of quercetin on gingival regeneration. Methods: Cell viability and proliferation capacity were tested by CCK-8 kit. Cell migration was monitored using Transwell chambers. An inflammatory situation was mimicked using Pg. LPS. RT-qPCR was performed to measure mRNA levels of IL-β1, TNF-α, TGF-β1, and TGF-β3. Protein levels of TGF-β1 and TGF-β3 were evaluated with ELISA. Results: 20 μmol/L quercetin enhanced the proliferation and migration of HOKs. Furthermore, 20 μmol/L quercetin increased both mRNA and protein levels of TGF-β3 under inflammatory conditions while 50 μmol/L quercetin increased expression of TGF-β1. Expression of IL-β1 and TNF-α were downregulated by quercetin treatment. Conclusion: Compared with untreated controls, the re-epithelialization of 20 μmol/L quercetin-treated HOKs were highly rapid.

米娅莎尔·呼加合买提,李雪,吕慧欣,丁鑫鑫,周延民. 槲皮素对人口腔上皮细胞再生的生物学效应研究[J]. 口腔医学研究, 2018, 34(9): 991-994.

Miyashaer·Hujiahemaiti, LI Xue, LV Hui-xin, DING Xin-xin, ZHOU Yan-min.. Effects of Quercetin on Human Oral Keratinocyte Regeneration.[J]. Journal of Oral Science Research, 2018, 34(9): 991-994.

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槲皮素对人口腔上皮细胞再生的生物学效应研究

Effects of Quercetin on Human Oral Keratinocyte Regeneration.

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