口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (11): 1212-1216.DOI: 10.13701/j.cnki.kqyxyj.2018.11.016

• 口腔肿瘤学研究 • 上一篇    下一篇

STAT6在涎腺腺样囊性癌组织中的表达及对细胞增殖和侵袭的影响

裴浩*, 夏冬景, 黄莹莹   

  1. 河南省南阳医学高等专科学校第一附属医院口腔科 河南 南阳 473058
  • 收稿日期:2018-03-29 出版日期:2018-11-28 发布日期:2018-11-23
  • 通讯作者: 裴浩,E-mail:3121274260@qq.com
  • 作者简介:裴浩(1969~ ),男,河南南阳人,学士,副主任医师,主要从事口腔颌面外科的临床治疗工作。

Expression of STAT6 in Salivary Gland Adenoid Cystic Carcinoma and Its Effect on Cell Proliferation and Invasion

PEI Hao*, XIA Dong-jing, HUANG Ying-ying   

  1. Department of Stomatology, The First Affiliated Hospital of Nanyang Medical College, Nanyang 473058, China.
  • Received:2018-03-29 Online:2018-11-28 Published:2018-11-23

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摘要： 目的:探讨STAT6在涎腺腺样囊性癌(SACC)组织中的表达,以及下调STAT6基因表达对细胞增殖和侵袭的影响。方法:选取SACC患者53例,并留取涎腺良性肿瘤正常涎腺腺组织40例作为对照组,免疫组化法检测SACC和对照组组织中STAT6蛋白表达,培养ACC-2细胞,分为STAT6干扰序列组、阴性对照组和空白组,STAT6干扰序列组采用小分子干扰RNA(siRNA)技术下调细胞中STAT6基因表达,实时荧光定量PCR技术检测STAT6基因表达,MTT比色法检测细胞增殖能力,Transwell法检测细胞迁移和侵袭能力。结果:SACC组织中STAT6蛋白阳性表达率为71.70%,高于对照组的28.30%(χ2=19.962,P=0.000);SACC组织中STAT6蛋白阳性表达与TNM分期、淋巴结转移和远处转移有关(P<0.05);STAT6干扰序列组细胞中STAT6 mRNA相对表达量低于阴性对照组和空白组(P<0.05);MTT实验结果显示,STAT6干扰序列组24~96 h细胞A值低于阴性对照组和空白组(P<0.05);STAT6干扰序列组迁移细胞数和侵袭细胞数低于阴性对照组和空白组(P<0.05)。结论:SACC组织中STAT6蛋白呈高表达,下调STAT6基因表达可抑制细胞增殖、迁移及侵袭。

关键词: 涎腺腺样囊性癌, 信号转导与转录激活因子6, 细胞增殖, 细胞侵袭

Abstract: Objective: To investigate the expression of STAT6 in salivary gland adenoid cystic carcinoma (SACC), and the effects of down-regulation of STAT6 gene expression on cell proliferation and invasion. Methods: A total of 53 cases of SACC were selected. And 40 cases of normal gland tissues from benign parotid gland tumors were taken as the control group. Immunohistochemical method was used to detect the expressions of STAT6 proteins in SACC and adjacent tissues. The ACC-2 cells were cultured and divided into STAT6 interference sequence group, negative control group, and blank group. Small interfering RNA (siRNA) technology was used to down-regulate expression of STAT6 gene of cells in STAT6 interference sequence group. Real-time fluorescence quantitative PCR was used to detect the expression of STAT6 gene in cells. MTT colorimetric assay was used to detect cell proliferation. Transwell assay was used to detect cell migration and invasion. Results: The positive expression rate of STAT6 protein in SACC was 71.70%, which was higher than that of control group (P=0.000). The positive expression of STAT6 protein in SACC was related to TNM stage, lymph node metastasis, and distant metastasis (P<0.05). The relative expression level of STAT6 mRNA in cells in STAT6 interference sequence group was lower than that in negative control group and blank group (P<0.05). The absorbance values at 24-96h of cells in STAT6 interference sequence group was lower than those in negative control group and blank group (P<0.05). The number of migration cells and the number of invasive cells in STAT6 interference sequence group were lower than those in negative control group and blank group (P<0.05). Conclusion: The expression of STAT6 protein in the SACC tissues was high. Down-regulation of STAT6 gene expression could inhibit the cell proliferation, migration, and invasion.

Key words: Salivary gland adenoid cystic carcinoma, Signal transducer and activator of transcription 6, Cell proliferation, Cell invasion