口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (1): 6-9.DOI: 10.13701/j.cnki.kqyxyj.2018.01.002

• 口腔干细胞研究 • 上一篇    下一篇

BaTO3/P(VDF-TrFE)纳米复合膜对干细胞铺展与增殖的影响

李毅萍1,2, 戴小寒1, 唐瞻贵1, 张学慧2*   

  1. 1. 中南大学湘雅口腔医学院修复科 湖南 长沙 410078;
    2. 北京大学口腔医学院口腔材料研究室/口腔医疗器械检验中心 北京 100081
  • 收稿日期:2017-07-10 出版日期:2018-01-28 发布日期:2018-01-26
  • 通讯作者: 张学慧,E-mail: zhangxuehui914@163.com
  • 作者简介:李毅萍(1974~ ),女,湖南湘潭人,博士在读,主要从事口腔修复学研究工作。
  • 基金资助:
    国家自然科学基金资助项目(编号:51502006)

Effect of BaTO3/P (VDF-TrFE) Nanocomposite Membrane on Proliferation and Proliferation of Stem Cells

LI Yi-ping1,2, DAI Xiao-han1, TANG Zhan-gui1, ZHANG Xue-hui2*   

  1. 1. Department of Prosthodontics, Xiangya School and Hospital of Stomatology, Central South University, Changsha 410078, China;
    2. Department of Dental Materials & Dental Medical Devices Testing Center, Peking University School and Hospital of Stomatology, Beijing 100081, China
  • Received:2017-07-10 Online:2018-01-28 Published:2018-01-26

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摘要： 目的:对比考察不同钛酸钡含量的BaTO3/P(VDF-TrFE)压电纳米复合膜对大鼠骨髓间充质干细胞粘附、铺展和增殖活性的影响,为优化电活性引导组织再生膜材料的生物学性能提供基础。方法:采用溶液浇铸法制备出含有不同钛酸钡纳米颗粒含量(0vol%、1vol%、3vol%、5vol%)的BaTO3/P(VDF-TrFE)压电纳米复合膜,通过扫描电镜、原子力显微镜和X-射线衍射仪对材料表面形貌和化学成分进行检测,采用铁电测试仪对材料的压电性能进行测试。取P3-P5代大鼠BMSCs于极化处理后的含有不同钛酸钡BTO含量的BaTO3/P(VDF-TrFE)压电纳米复合膜上常规培养,分别用扫描电镜和CCK-8法检测BMSCs的粘附铺展和增殖情况。采用SPSS13.0软件,运用单因素方差分析进行统计学分析。结果:压电纳米复合膜的压电性能呈现钛酸钡含量依赖性。结论:含有5vol%钛酸钡纳米颗粒的BaTO3/P(VDF-TrFE)压电纳米复合膜有利于促进BMSCs的粘附、铺展和增殖。

关键词: 纳米复合膜, 压电活性, 干细胞, 细胞铺展, 细胞增殖

Abstract: Objective: To investigate the effect of BaTO3/P(VDF-TrFE) piezoelectric nanocomposite membrane with different barium titanate contents on the adhesion, spreading, and proliferation of rat bone marrow mesenchymal stem cells to provide the basis for optimizing the biological properties of the electroactive materials. Methods: BaTO3/P(VDF-TrFE) piezoelectric nanocomposite films containing different contents of barium titanate nanoparticles (0vol%, 1vol%, 3vol%, 5vol%) were prepared by solution casting method. The surface morphology and microstructures were characterized by scanning electron microscopy and atomic force microscopy. X-ray diffraction was used to detect the chemical composition of the materials. The ferroelectric tester was used to test the piezoelectric properties of the materials. The rat BMSCs of P3-P5 generation were cultured on BaTO3/P (VDF-TrFE) piezoelectric nanocomposites containing different barium titanate after polarization treatment. The adhesion spread and proliferation of BMSCs were detected by scanning electron microscopy and CCK-8, respectively. SPSS13.0 software was used to analyze the statistical analysis by one-way ANOVA. Results: The piezoelectric properties of nanocomposite films showed barium titanate content dependence. Conclusion: BaTO3/P(VDF-TrFE) piezoelectric nanocomposite films containing 5 vol% barium titanate nanoparticles can promote the adhesion, spreading, and proliferation of BMSCs.

Key words: Nanocomposite films, Piezoelectric activity, Stem cells, Cell spreading, Cell proliferation

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