初级纤毛对人牙周膜细胞成骨相关基因表达的影响

doi:10.13701/j.cnki.kqyxyj.2018.01.003

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (1): 10-13.DOI: 10.13701/j.cnki.kqyxyj.2018.01.003

初级纤毛对人牙周膜细胞成骨相关基因表达的影响

蒋思琪¹, 尹凤英², 张璠³, 王敏¹, 夏海斌^1*

1. 武汉大学口腔医学院种植科湖北武汉 430079;
2. 四川大学华西口腔医学院四川成都 610041;
3. 中国科学院水生生物研究所湖北武汉 430070

收稿日期:2017-08-03 出版日期:2018-01-28 发布日期:2018-01-26
通讯作者: 夏海斌,E-mail:xhaibin@whu.edu.cn
作者简介:蒋思琪(1993~ ),女,湖北荆门人,硕士在读,主要从事口腔基础及临床的研究工作。
基金资助:
国家自然科学基金资助项目(编号:81271179)

Effect of Primary Cilia on Expression of Osteogenic-related Genes of Human Periodontal Ligament Cells

JIANG Si-qi¹, YIN Feng-ying², ZHANG Fan³, WANG Min¹, XIA Hai-bin^1*

1. School of Stomatology, Wuhan University, Wuhan 430079, China;
2. West China College of Stomatology, Sichuan University, Chengdu 610041, China;
3. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430070, China

Received:2017-08-03 Online:2018-01-28 Published:2018-01-26

摘要/Abstract

摘要： 目的:通过沉默纤毛运输蛋白88(intraflagellar transport 88,IFT88)基因抑制人牙周膜细胞(human periodontal ligament cells, hPDLCs)中初级纤毛的形成,进一步研究其对hPDLCs成骨相关基因表达的影响。方法:实验分为对照组和shIFT88组。对照组感染无意序列,shIFT88组感染含IFT88基因有效短发卡RNA(short hairpin RNA,shRNA)的慢病毒。qRT-PCR、Western blot检测各组细胞中IFT88表达情况,免疫荧光检测初级纤毛形成, qRT-PCR检测成骨相关基因COL1A1,OCN,Runx2,BMP2表达量的变化。结果:与对照组相比,shIFT88组IFT88表达量明显下调(P<0.01),纤毛率降低(P<0.01),成骨相关基因COL1A,OCN,Runx2,BMP2基础表达量明显下调(P<0.01)。结论:沉默IFT88基因后,hPDLCs初级纤毛形成受阻,成骨相关基因表达量明显下调。

关键词: 纤毛运输蛋白88, 初级纤毛, 人牙周膜细胞, 成骨相关基因

Abstract: Objective: To study the expression of osteogenic-related genes of human periodontal ligament cells (hPDLCs) by knocking down IFT88 to inhibit the formation of primary cilia. Methods: Control shRNA and shIFT88 were infected into hPDLCs. The expression of IFT88 was detected by qRT-PCR and Western blot. The formation of primary cilia was detected by immunofluorescence and the expressions of osteogenic-related genes COL1A1, OCN, Runx2, and BMP2 were detected by qRT-PCR. Results: The PKLO.1-shIFT88 was stably expressed in hPDLCs and inhibited the expression of IFT88 effectively (P<0.01). The formation of primary cilia was partly suppressed (P<0.01). The expressions of osteogenic-related genes like COL1A1, OCN, Runx2, and BMP2 were decreased significantly (P<0.01). Conclusion: Knockdown IFT88 inhibited the formation of primary cilia and reduced the expressions of osteogenic-related genes of hPDLCs.

Key words: Intraflagellar transport 88, Primary cilia, Human periodontal ligament cells, Osteogenic-related genes

中图分类号:

R781.4

蒋思琪, 尹凤英, 张璠, 王敏, 夏海斌. 初级纤毛对人牙周膜细胞成骨相关基因表达的影响[J]. 口腔医学研究, 2018, 34(1): 10-13.

JIANG Si-qi, YIN Feng-ying, ZHANG Fan, WANG Min, XIA Hai-bin. Effect of Primary Cilia on Expression of Osteogenic-related Genes of Human Periodontal Ligament Cells[J]. Journal of Oral Science Research, 2018, 34(1): 10-13.

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初级纤毛对人牙周膜细胞成骨相关基因表达的影响

Effect of Primary Cilia on Expression of Osteogenic-related Genes of Human Periodontal Ligament Cells

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