口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (1): 14-17.DOI: 10.13701/j.cnki.kqyxyj.2018.01.004

• 口腔干细胞研究 • 上一篇    下一篇

SSUH2在小鼠牙胚发育中的时空表达

陈栋*, 鲁方丽, 李晓聪, 王莹莹, 李强   

  1. 郑州大学第一附属医院口腔科 河南 郑州 450052
  • 收稿日期:2017-10-10 出版日期:2018-01-28 发布日期:2018-01-26
  • 通讯作者: 陈栋,E-mail:chendongfmmu@163.com
  • 作者简介:陈栋(1970~ ),男,河南人,教授,博士,主要从事牙周病的治疗及口腔遗传病家系研究。
  • 基金资助:
    国家自然科学基金资助项目(编号:81470033)

Expressions of SSUH2 during Mouse Tooth Germ Development

CHEN Dong*, LU Fang-li, LI Xiao-cong, WANG Ying-ying, LI Qiang   

  1. Department of Stomatology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
  • Received:2017-10-10 Online:2018-01-28 Published:2018-01-26

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摘要： 目的:探讨牙本质发育不良I型(dentin dyspepsia type I,DD-I)相关致病基因Ssuh2在小鼠牙胚发育各个时期中的作用。方法:采用HE确定小鼠下颌第一磨牙牙胚发育E13.5d、E14.5d、E16.5d、P2.5d、P7d的时期,采用免疫组化法观察SSUH2在各个时期的表达分布。结果:蕾状期(E13.5d),SSUH2在上皮细胞和间充质细胞均呈阳性表达;帽状期(E14.5d)和钟状早期(E16.5d),SSUH2均在基底膜、内、外釉上皮层、釉结、颈环呈强阳性表达。细胞分化及分泌期(P2.5d)和矿化期(P7d),SSUH2均在成釉细胞、成牙本质细胞、牙釉质、上皮根鞘中呈强阳性表达。结论:SSUH2在小鼠牙胚发育中均有表达,且各个时期略有差异,SSUH2可能参与小鼠的牙胚发育。

关键词: SSUH2, 牙本质发育不良, 牙胚发育, 免疫组织化学

Abstract: Objective: To investigate the role of Ssuh2 associated with dentin dyspepsia type I (DD-I) in the period of tooth germ development in mice. Methods: The time of E13.5d, E14.5d, E16.5d, P2.5d, and P7d were determined by HE staining. The expression of SSUH2 was observed by immunohistochemistry. Results: During the bud stage (E13.5d), SSUH2 was positively expressed in epithelial cells and mesenchymal cells. During the cup period (E14.5d) and the early bell stage (E16.5d), SSUH2 was strongly positively expressed in the basement membrane, internal and external epithelium, enamel knot, and cervical loop. During the cell differentiation and secretory stage (P2.5d) and mineralization stage (P7d), SSUH2 was strongly expressed in ameloblasts, odontoblasts, enamel, and epithelium root sheath. Conclusion: SSUH2 was expressed during all stages of mouse tooth germ, however, the expression of SSUH2 was slightly different in each period. SSUH2 might involve in the development of tooth germs in mice.

Key words: SSUH2, Dentin dysplasia, Tooth germ development, Immunochemistry

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